Effects of intra-dentate gyrus microinjection of myokine irisin on long-term potentiation in male rats
ABSTRACT Induction of long-term potentiation (LTP) increases the storage capacity of synapses in the hippocampal dentate gyrus (DG). Irisin is a myokine generated from FNDC5 (a gene precursor) during exercise. Although intra-cornu ammonis 1 administration of irisin fortifies LTP in mice with Alzheim...
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Thieme Revinter Publicações
2020-01-01
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| Series: | Arquivos de Neuro-Psiquiatria |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0004-282X2019001200881&tlng=en |
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| author | Saeed Mohammadi Shahrbanoo Oryan Alireza Komaki Akram Eidi Mohammad Zarei |
| author_facet | Saeed Mohammadi Shahrbanoo Oryan Alireza Komaki Akram Eidi Mohammad Zarei |
| author_sort | Saeed Mohammadi |
| collection | DOAJ |
| description | ABSTRACT Induction of long-term potentiation (LTP) increases the storage capacity of synapses in the hippocampal dentate gyrus (DG). Irisin is a myokine generated from FNDC5 (a gene precursor) during exercise. Although intra-cornu ammonis 1 administration of irisin fortifies LTP in mice with Alzheimer's disease, the effects of intra-DG injection of irisin on the LTP in rats remains to be elucidated in vivo. In this study, male Wistar rats were randomly divided into a control group (saline), irisin (0.5, 1, and 1.5 μg/rat), and dimethyl sulfoxide (DMSO). After treatment, the population spike (PS) amplitude and slope of excitatory postsynaptic potentials (EPSP) were measured in the DG of rats in vivo. Moreover, following completion of the experiments, the stimulating and recording sites in the hippocampus were confirmed histologically from brain sections. Furthermore, biochemical assays like malondialdehyde (MDA), total antioxidant capacity (TAC), and total oxidant status (TOS) were evaluated (the antioxidant markers were analyzed in the plasma). Our results suggest that all doses of irisin (0.5, 1, 1.5 μg/rat) caused an increase in the EPSP slope and PS amplitude when compared with the control group. In addition, the results obtained showed that irisin decreased TOS and MDA levels while increasing TAC levels as a marker of lipid peroxidation in plasma. The present report provides direct evidence that irisin affects the activity-dependent synaptic plasticity in the dentate gyrus. |
| format | Article |
| id | doaj-art-e82d2a1d27474bd3bb9e4defec958196 |
| institution | Kabale University |
| issn | 1678-4227 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Thieme Revinter Publicações |
| record_format | Article |
| series | Arquivos de Neuro-Psiquiatria |
| spelling | doaj-art-e82d2a1d27474bd3bb9e4defec9581962025-08-20T03:24:48ZengThieme Revinter PublicaçõesArquivos de Neuro-Psiquiatria1678-42272020-01-01771288188710.1590/0004-282x20190184Effects of intra-dentate gyrus microinjection of myokine irisin on long-term potentiation in male ratsSaeed Mohammadihttps://orcid.org/0000-0001-6966-7493Shahrbanoo Oryanhttps://orcid.org/0000-0003-1930-2343Alireza Komakihttps://orcid.org/0000-0002-4161-4311Akram Eidihttps://orcid.org/0000-0002-6673-5347Mohammad Zareihttps://orcid.org/0000-0003-4034-3043ABSTRACT Induction of long-term potentiation (LTP) increases the storage capacity of synapses in the hippocampal dentate gyrus (DG). Irisin is a myokine generated from FNDC5 (a gene precursor) during exercise. Although intra-cornu ammonis 1 administration of irisin fortifies LTP in mice with Alzheimer's disease, the effects of intra-DG injection of irisin on the LTP in rats remains to be elucidated in vivo. In this study, male Wistar rats were randomly divided into a control group (saline), irisin (0.5, 1, and 1.5 μg/rat), and dimethyl sulfoxide (DMSO). After treatment, the population spike (PS) amplitude and slope of excitatory postsynaptic potentials (EPSP) were measured in the DG of rats in vivo. Moreover, following completion of the experiments, the stimulating and recording sites in the hippocampus were confirmed histologically from brain sections. Furthermore, biochemical assays like malondialdehyde (MDA), total antioxidant capacity (TAC), and total oxidant status (TOS) were evaluated (the antioxidant markers were analyzed in the plasma). Our results suggest that all doses of irisin (0.5, 1, 1.5 μg/rat) caused an increase in the EPSP slope and PS amplitude when compared with the control group. In addition, the results obtained showed that irisin decreased TOS and MDA levels while increasing TAC levels as a marker of lipid peroxidation in plasma. The present report provides direct evidence that irisin affects the activity-dependent synaptic plasticity in the dentate gyrus.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0004-282X2019001200881&tlng=enDentate gyrusneuronal plasticitylong-term potentiation |
| spellingShingle | Saeed Mohammadi Shahrbanoo Oryan Alireza Komaki Akram Eidi Mohammad Zarei Effects of intra-dentate gyrus microinjection of myokine irisin on long-term potentiation in male rats Arquivos de Neuro-Psiquiatria Dentate gyrus neuronal plasticity long-term potentiation |
| title | Effects of intra-dentate gyrus microinjection of myokine irisin on long-term potentiation in male rats |
| title_full | Effects of intra-dentate gyrus microinjection of myokine irisin on long-term potentiation in male rats |
| title_fullStr | Effects of intra-dentate gyrus microinjection of myokine irisin on long-term potentiation in male rats |
| title_full_unstemmed | Effects of intra-dentate gyrus microinjection of myokine irisin on long-term potentiation in male rats |
| title_short | Effects of intra-dentate gyrus microinjection of myokine irisin on long-term potentiation in male rats |
| title_sort | effects of intra dentate gyrus microinjection of myokine irisin on long term potentiation in male rats |
| topic | Dentate gyrus neuronal plasticity long-term potentiation |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0004-282X2019001200881&tlng=en |
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