Simultaneous Determination of Volatile Constituents from Acorus tatarinowii Schott in Rat Plasma by Gas Chromatography-Mass Spectrometry with Selective Ion Monitoring and Application in Pharmacokinetic Study
A sensitive and specific gas chromatographic-mass spectrometry with selected ion monitoring (GC-MS/SIM) method has been developed for simultaneous identification and quantification of α-asarone, β-asarone, and methyl eugenol of Acorus tatarinowii Schott in rat plasma. Chromatographic separation was...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2013/949830 |
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| author | Xue Meng Xinfeng Zhao Shixiang Wang Pu Jia Yajun Bai Sha Liao Xiaohui Zheng |
| author_facet | Xue Meng Xinfeng Zhao Shixiang Wang Pu Jia Yajun Bai Sha Liao Xiaohui Zheng |
| author_sort | Xue Meng |
| collection | DOAJ |
| description | A sensitive and specific gas chromatographic-mass spectrometry with selected ion monitoring (GC-MS/SIM) method has been developed for simultaneous identification and quantification of α-asarone, β-asarone, and methyl eugenol of Acorus tatarinowii Schott in rat plasma. Chromatographic separation was performed on a Restek Rxi-5MS capillary column (30 m × 0.32 mm × 0.25 μm), using 1-naphthol as internal standard (IS). MS detection of these compounds and IS was performed at m/z 178, 208, 208, and 144. Intra- and interday precisions of all compounds of interest were less than 10%. The recoveries are situated in the range of 92.4–105.2%. Pharmacokinetics of methyl eugenol confirmed to be one-compartment open model, α-asarone and β-asarone was two-compartment open model, respectively. The method will probably be an alternative to simultaneous determination and pharmacokinetic study of volatile ingredients in Acorus tatarinowii Schott. |
| format | Article |
| id | doaj-art-e8298a5752ec49119a0df9e0bb6a6a5c |
| institution | DOAJ |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-e8298a5752ec49119a0df9e0bb6a6a5c2025-08-20T03:21:03ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732013-01-01201310.1155/2013/949830949830Simultaneous Determination of Volatile Constituents from Acorus tatarinowii Schott in Rat Plasma by Gas Chromatography-Mass Spectrometry with Selective Ion Monitoring and Application in Pharmacokinetic StudyXue Meng0Xinfeng Zhao1Shixiang Wang2Pu Jia3Yajun Bai4Sha Liao5Xiaohui Zheng6College of Life Sciences, Northwest University, P.O. Box 195, No. 229 Taibai North Road, Xi’an 710069, ChinaCollege of Life Sciences, Northwest University, P.O. Box 195, No. 229 Taibai North Road, Xi’an 710069, ChinaCollege of Life Sciences, Northwest University, P.O. Box 195, No. 229 Taibai North Road, Xi’an 710069, ChinaCollege of Life Sciences, Northwest University, P.O. Box 195, No. 229 Taibai North Road, Xi’an 710069, ChinaCollege of Life Sciences, Northwest University, P.O. Box 195, No. 229 Taibai North Road, Xi’an 710069, ChinaCollege of Life Sciences, Northwest University, P.O. Box 195, No. 229 Taibai North Road, Xi’an 710069, ChinaCollege of Life Sciences, Northwest University, P.O. Box 195, No. 229 Taibai North Road, Xi’an 710069, ChinaA sensitive and specific gas chromatographic-mass spectrometry with selected ion monitoring (GC-MS/SIM) method has been developed for simultaneous identification and quantification of α-asarone, β-asarone, and methyl eugenol of Acorus tatarinowii Schott in rat plasma. Chromatographic separation was performed on a Restek Rxi-5MS capillary column (30 m × 0.32 mm × 0.25 μm), using 1-naphthol as internal standard (IS). MS detection of these compounds and IS was performed at m/z 178, 208, 208, and 144. Intra- and interday precisions of all compounds of interest were less than 10%. The recoveries are situated in the range of 92.4–105.2%. Pharmacokinetics of methyl eugenol confirmed to be one-compartment open model, α-asarone and β-asarone was two-compartment open model, respectively. The method will probably be an alternative to simultaneous determination and pharmacokinetic study of volatile ingredients in Acorus tatarinowii Schott.http://dx.doi.org/10.1155/2013/949830 |
| spellingShingle | Xue Meng Xinfeng Zhao Shixiang Wang Pu Jia Yajun Bai Sha Liao Xiaohui Zheng Simultaneous Determination of Volatile Constituents from Acorus tatarinowii Schott in Rat Plasma by Gas Chromatography-Mass Spectrometry with Selective Ion Monitoring and Application in Pharmacokinetic Study Journal of Analytical Methods in Chemistry |
| title | Simultaneous Determination of Volatile Constituents from Acorus tatarinowii Schott in Rat Plasma by Gas Chromatography-Mass Spectrometry with Selective Ion Monitoring and Application in Pharmacokinetic Study |
| title_full | Simultaneous Determination of Volatile Constituents from Acorus tatarinowii Schott in Rat Plasma by Gas Chromatography-Mass Spectrometry with Selective Ion Monitoring and Application in Pharmacokinetic Study |
| title_fullStr | Simultaneous Determination of Volatile Constituents from Acorus tatarinowii Schott in Rat Plasma by Gas Chromatography-Mass Spectrometry with Selective Ion Monitoring and Application in Pharmacokinetic Study |
| title_full_unstemmed | Simultaneous Determination of Volatile Constituents from Acorus tatarinowii Schott in Rat Plasma by Gas Chromatography-Mass Spectrometry with Selective Ion Monitoring and Application in Pharmacokinetic Study |
| title_short | Simultaneous Determination of Volatile Constituents from Acorus tatarinowii Schott in Rat Plasma by Gas Chromatography-Mass Spectrometry with Selective Ion Monitoring and Application in Pharmacokinetic Study |
| title_sort | simultaneous determination of volatile constituents from acorus tatarinowii schott in rat plasma by gas chromatography mass spectrometry with selective ion monitoring and application in pharmacokinetic study |
| url | http://dx.doi.org/10.1155/2013/949830 |
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