Molecular detection of phenol-soluble modulin-mec (PSM-mec) in Staphylococcus aureus clinical isolates from Federal Medical Center Birnin Kebbi, North-West, Nigeria
Aim: This study was carried out to isolate and detect virulence genes associated with Staphylococcus (S.) aureus clinical isolates from the Federal Medical Center Birnin Kebbi, Nigeria. Methods: To obtain S. aureus isolates, samples were taken from urine, sputum, blood and wound sources. S. aureus...
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| Format: | Article |
| Language: | deu |
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German Medical Science GMS Publishing House
2025-03-01
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| Series: | GMS Hygiene and Infection Control |
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| Online Access: | https://journals.publisso.de/en/journals/hic/volume20/dgkh000538 |
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| author | Maishanu, Isah Musa Gbonjubola, Adeshina O. Mujahid, Hussaini Olayinka, Busayo O. |
| author_facet | Maishanu, Isah Musa Gbonjubola, Adeshina O. Mujahid, Hussaini Olayinka, Busayo O. |
| author_sort | Maishanu, Isah Musa |
| collection | DOAJ |
| description | Aim: This study was carried out to isolate and detect virulence genes associated with Staphylococcus (S.) aureus clinical isolates from the Federal Medical Center Birnin Kebbi, Nigeria.
Methods: To obtain S. aureus isolates, samples were taken from urine, sputum, blood and wound sources. S. aureus was phenotypically identified using Microgen staph ID system and PSM-mec and PVL genes were detected using polymerase chain reaction (PCR).
Results: A total of 48 non-duplicate S. aureus isolates were obtained (21 from wound swabs, 7 from blood, 15 from urine, and 5 from sputum). From the 14 S. aureus isolates examined by PCR, the most abundant gene was PSM-mec (42.8%), while the PVL was the least abundant with 21.4%.
Conclusion: Because it gives highly specific and accurate results, it is essential to use the PCR technique to detect S. aureus virulence determinants as well as PSM-mec and PVL as targets for antimicrobial agents. |
| format | Article |
| id | doaj-art-e8292c3cfe414500a65a21e1b989eb51 |
| institution | DOAJ |
| issn | 2196-5226 |
| language | deu |
| publishDate | 2025-03-01 |
| publisher | German Medical Science GMS Publishing House |
| record_format | Article |
| series | GMS Hygiene and Infection Control |
| spelling | doaj-art-e8292c3cfe414500a65a21e1b989eb512025-08-20T02:57:00ZdeuGerman Medical Science GMS Publishing HouseGMS Hygiene and Infection Control2196-52262025-03-0120Doc0910.3205/dgkh000538Molecular detection of phenol-soluble modulin-mec (PSM-mec) in Staphylococcus aureus clinical isolates from Federal Medical Center Birnin Kebbi, North-West, NigeriaMaishanu, Isah Musa0Gbonjubola, Adeshina O.1Mujahid, Hussaini2Olayinka, Busayo O.3Kebbi State University of Science and Technology Aliero, NigeriaAhmadu Bello University Zaria, Kaduna, NigeriaAhmadu Bello University Zaria, Kaduna, NigeriaAhmadu Bello University Zaria, Kaduna, NigeriaAim: This study was carried out to isolate and detect virulence genes associated with Staphylococcus (S.) aureus clinical isolates from the Federal Medical Center Birnin Kebbi, Nigeria. Methods: To obtain S. aureus isolates, samples were taken from urine, sputum, blood and wound sources. S. aureus was phenotypically identified using Microgen staph ID system and PSM-mec and PVL genes were detected using polymerase chain reaction (PCR). Results: A total of 48 non-duplicate S. aureus isolates were obtained (21 from wound swabs, 7 from blood, 15 from urine, and 5 from sputum). From the 14 S. aureus isolates examined by PCR, the most abundant gene was PSM-mec (42.8%), while the PVL was the least abundant with 21.4%. Conclusion: Because it gives highly specific and accurate results, it is essential to use the PCR technique to detect S. aureus virulence determinants as well as PSM-mec and PVL as targets for antimicrobial agents.https://journals.publisso.de/en/journals/hic/volume20/dgkh000538staphylococcus aureusphenol soluble modulin-mecpanton valentine leucocidinvirulence genes |
| spellingShingle | Maishanu, Isah Musa Gbonjubola, Adeshina O. Mujahid, Hussaini Olayinka, Busayo O. Molecular detection of phenol-soluble modulin-mec (PSM-mec) in Staphylococcus aureus clinical isolates from Federal Medical Center Birnin Kebbi, North-West, Nigeria GMS Hygiene and Infection Control staphylococcus aureus phenol soluble modulin-mec panton valentine leucocidin virulence genes |
| title | Molecular detection of phenol-soluble modulin-mec (PSM-mec) in Staphylococcus aureus clinical isolates from Federal Medical Center Birnin Kebbi, North-West, Nigeria |
| title_full | Molecular detection of phenol-soluble modulin-mec (PSM-mec) in Staphylococcus aureus clinical isolates from Federal Medical Center Birnin Kebbi, North-West, Nigeria |
| title_fullStr | Molecular detection of phenol-soluble modulin-mec (PSM-mec) in Staphylococcus aureus clinical isolates from Federal Medical Center Birnin Kebbi, North-West, Nigeria |
| title_full_unstemmed | Molecular detection of phenol-soluble modulin-mec (PSM-mec) in Staphylococcus aureus clinical isolates from Federal Medical Center Birnin Kebbi, North-West, Nigeria |
| title_short | Molecular detection of phenol-soluble modulin-mec (PSM-mec) in Staphylococcus aureus clinical isolates from Federal Medical Center Birnin Kebbi, North-West, Nigeria |
| title_sort | molecular detection of phenol soluble modulin mec psm mec in staphylococcus aureus clinical isolates from federal medical center birnin kebbi north west nigeria |
| topic | staphylococcus aureus phenol soluble modulin-mec panton valentine leucocidin virulence genes |
| url | https://journals.publisso.de/en/journals/hic/volume20/dgkh000538 |
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