Molecular detection of phenol-soluble modulin-mec (PSM-mec) in Staphylococcus aureus clinical isolates from Federal Medical Center Birnin Kebbi, North-West, Nigeria
Aim: This study was carried out to isolate and detect virulence genes associated with Staphylococcus (S.) aureus clinical isolates from the Federal Medical Center Birnin Kebbi, Nigeria. Methods: To obtain S. aureus isolates, samples were taken from urine, sputum, blood and wound sources. S. aureus...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | deu |
| Published: |
German Medical Science GMS Publishing House
2025-03-01
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| Series: | GMS Hygiene and Infection Control |
| Subjects: | |
| Online Access: | https://journals.publisso.de/en/journals/hic/volume20/dgkh000538 |
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| Summary: | Aim: This study was carried out to isolate and detect virulence genes associated with Staphylococcus (S.) aureus clinical isolates from the Federal Medical Center Birnin Kebbi, Nigeria.
Methods: To obtain S. aureus isolates, samples were taken from urine, sputum, blood and wound sources. S. aureus was phenotypically identified using Microgen staph ID system and PSM-mec and PVL genes were detected using polymerase chain reaction (PCR).
Results: A total of 48 non-duplicate S. aureus isolates were obtained (21 from wound swabs, 7 from blood, 15 from urine, and 5 from sputum). From the 14 S. aureus isolates examined by PCR, the most abundant gene was PSM-mec (42.8%), while the PVL was the least abundant with 21.4%.
Conclusion: Because it gives highly specific and accurate results, it is essential to use the PCR technique to detect S. aureus virulence determinants as well as PSM-mec and PVL as targets for antimicrobial agents. |
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| ISSN: | 2196-5226 |