Study on Ligninase Activity of Aspergillus fumigatus G13 Induced by Lignin Model Compounds
With the ligninase activity as an index, three model compounds, ferulic acid, pcoumaric acid and sinapic acid, were used as substrates to study the characteristics of Aspergillus fumigatus G13 induced enzyme production by adding cellulose and glucose and changing the culture mode The results showed...
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| Main Authors: | , |
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| Format: | Article |
| Language: | zho |
| Published: |
Harbin University of Science and Technology Publications
2020-04-01
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| Series: | Journal of Harbin University of Science and Technology |
| Subjects: | |
| Online Access: | https://hlgxb.hrbust.edu.cn/#/digest?ArticleID=1764 |
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| Summary: | With the ligninase activity as an index, three model compounds, ferulic acid, pcoumaric acid and sinapic acid, were used as substrates to study the characteristics of Aspergillus fumigatus G13 induced enzyme production by adding cellulose and glucose and changing the culture mode The results showed that the ability of producing enzymes in shake flask culture was 2-5 times higher than that in static culture The lignin model compound was used as inducer alone, and the induction effect of sinapic acid was the strongest The maximum activities of LiP, Lac and MnP were 2408, 2167 and 11078U/L, respectively Carboxymethyl cellulose (CMC), microcrystalline cellulose (MCC) and glucose as cosubstrates all promoted the induction ability of the three model compounds to the strain Among them, glucose was the most prominent (about 138-534 times higher than the original enzyme activity), and CMC was better than microcrystalline cellulose Cellulose model compounds and glucose played a synergistic role in the induction of enzyme production |
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| ISSN: | 1007-2683 |