Genome-wide identification and characterization of transcription factors involved in defense responses against Sclerotinia sclerotiorum in Brassica juncea
Abstract Sclerotinia sclerotiorum could cause significant yield losses of up to 70% in rapeseed cultivation. Nevertheless, the availability of immune or highly resistant germplasm and mechanisms to combat S. sclerotiorum, particularly in B. juncea, remains insufficient. Transcription factors (TFs) a...
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Nature Portfolio
2025-02-01
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| author | Xu Yang Shaomin Guo Hairun Jin Jinze Zhang Lijing Xiao Qingjing Ouyang Xu Long Zhongbin Yan Entang Tian |
| author_facet | Xu Yang Shaomin Guo Hairun Jin Jinze Zhang Lijing Xiao Qingjing Ouyang Xu Long Zhongbin Yan Entang Tian |
| author_sort | Xu Yang |
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| description | Abstract Sclerotinia sclerotiorum could cause significant yield losses of up to 70% in rapeseed cultivation. Nevertheless, the availability of immune or highly resistant germplasm and mechanisms to combat S. sclerotiorum, particularly in B. juncea, remains insufficient. Transcription factors (TFs) are recognized for their critical role in plant defense against S. sclerotiorum. In the present study, a total of 4807 TFs from 48 families were expressed and identified at 12, 24, and 36 h after inoculation (HAI) in two B. juncea lines: G21-912, exhibiting higher S. sclerotiorum resistance (HR), and G21-853, displaying lower S. sclerotiorum resistance (LR). The number of differentially expressed TFs (DETs) between the HR and the LR lines peaked at 24 HAI, with 202 upregulated and 105 downregulated TFs. Through expression and subcellular localization analyses, three candidate DETs, namely BjuA037408 (ETHYLENE RESPONSIVE FACTOR 59, ERF59), BjuB028842 (RELATED TO ABI3/VP1 1, RAV1), and BjuA016484 (WRKY25), were identified as the primary TFs in defense against S. sclerotiorum inoculation. The expression of these three genes was validated in the double haploid lines of BC3 (the third backcrossing generation) derived from the HR×LR cross. This study serves as a valuable case study for the characterization of TFs associated with defense mechanisms against S. sclerotiorum in B. juncea. The confirmed resistant B. juncea line of HR, along with the three key DETs, is expected to significantly contribute to future breeding efforts aimed at developing Sclerotinia-resistant varieties. |
| format | Article |
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| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-02-01 |
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| spelling | doaj-art-e816a880ee504a9fb4b62e983deca7a62025-02-09T12:32:07ZengNature PortfolioScientific Reports2045-23222025-02-0115111310.1038/s41598-025-89054-5Genome-wide identification and characterization of transcription factors involved in defense responses against Sclerotinia sclerotiorum in Brassica junceaXu Yang0Shaomin Guo1Hairun Jin2Jinze Zhang3Lijing Xiao4Qingjing Ouyang5Xu Long6Zhongbin Yan7Entang Tian8Agricultural College of Guizhou University, Guizhou UniversityRapeseed Research Institute, Guizhou Academy of Agricultural SciencesAgricultural College of Guizhou University, Guizhou UniversityAgricultural College of Guizhou University, Guizhou UniversityAgricultural College of Guizhou University, Guizhou UniversityAgricultural College of Guizhou University, Guizhou UniversityAgricultural College of Guizhou University, Guizhou UniversityAgricultural College of Guizhou University, Guizhou UniversityAgricultural College of Guizhou University, Guizhou UniversityAbstract Sclerotinia sclerotiorum could cause significant yield losses of up to 70% in rapeseed cultivation. Nevertheless, the availability of immune or highly resistant germplasm and mechanisms to combat S. sclerotiorum, particularly in B. juncea, remains insufficient. Transcription factors (TFs) are recognized for their critical role in plant defense against S. sclerotiorum. In the present study, a total of 4807 TFs from 48 families were expressed and identified at 12, 24, and 36 h after inoculation (HAI) in two B. juncea lines: G21-912, exhibiting higher S. sclerotiorum resistance (HR), and G21-853, displaying lower S. sclerotiorum resistance (LR). The number of differentially expressed TFs (DETs) between the HR and the LR lines peaked at 24 HAI, with 202 upregulated and 105 downregulated TFs. Through expression and subcellular localization analyses, three candidate DETs, namely BjuA037408 (ETHYLENE RESPONSIVE FACTOR 59, ERF59), BjuB028842 (RELATED TO ABI3/VP1 1, RAV1), and BjuA016484 (WRKY25), were identified as the primary TFs in defense against S. sclerotiorum inoculation. The expression of these three genes was validated in the double haploid lines of BC3 (the third backcrossing generation) derived from the HR×LR cross. This study serves as a valuable case study for the characterization of TFs associated with defense mechanisms against S. sclerotiorum in B. juncea. The confirmed resistant B. juncea line of HR, along with the three key DETs, is expected to significantly contribute to future breeding efforts aimed at developing Sclerotinia-resistant varieties.https://doi.org/10.1038/s41598-025-89054-5Sclerotinia sclerotiorumTranscription factorsDETsBrassica juncea |
| spellingShingle | Xu Yang Shaomin Guo Hairun Jin Jinze Zhang Lijing Xiao Qingjing Ouyang Xu Long Zhongbin Yan Entang Tian Genome-wide identification and characterization of transcription factors involved in defense responses against Sclerotinia sclerotiorum in Brassica juncea Scientific Reports Sclerotinia sclerotiorum Transcription factors DETs Brassica juncea |
| title | Genome-wide identification and characterization of transcription factors involved in defense responses against Sclerotinia sclerotiorum in Brassica juncea |
| title_full | Genome-wide identification and characterization of transcription factors involved in defense responses against Sclerotinia sclerotiorum in Brassica juncea |
| title_fullStr | Genome-wide identification and characterization of transcription factors involved in defense responses against Sclerotinia sclerotiorum in Brassica juncea |
| title_full_unstemmed | Genome-wide identification and characterization of transcription factors involved in defense responses against Sclerotinia sclerotiorum in Brassica juncea |
| title_short | Genome-wide identification and characterization of transcription factors involved in defense responses against Sclerotinia sclerotiorum in Brassica juncea |
| title_sort | genome wide identification and characterization of transcription factors involved in defense responses against sclerotinia sclerotiorum in brassica juncea |
| topic | Sclerotinia sclerotiorum Transcription factors DETs Brassica juncea |
| url | https://doi.org/10.1038/s41598-025-89054-5 |
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