Analysis of OCT4 and PGP9.5 gene expression in prenatal and postnatal buffalo (Bubalus bubalis) testes
This study aimed to investigate and characterize the spermatogonial stem cells (SSCs) in buffaloes at different stages of development, including prenatal, neonatal, prepubertal, and adult testes. We sought a comprehensive understanding of these cells through a combination of histological, immunohist...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2024-12-01
|
| Series: | Animal Biotechnology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/10495398.2023.2285509 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849713912599019520 |
|---|---|
| author | Ashritha Q. Carlo Devendra Pathak Ratan K. Choudhary Opinder Singh Neelam Bansal |
| author_facet | Ashritha Q. Carlo Devendra Pathak Ratan K. Choudhary Opinder Singh Neelam Bansal |
| author_sort | Ashritha Q. Carlo |
| collection | DOAJ |
| description | This study aimed to investigate and characterize the spermatogonial stem cells (SSCs) in buffaloes at different stages of development, including prenatal, neonatal, prepubertal, and adult testes. We sought a comprehensive understanding of these cells through a combination of histological, immunohistochemical, and ultrastructural analyses. Specifically, we examined changes in the expression of two potential SSC markers, OCT4 and PGP9.5, using immunohistochemistry. Additionally, we conducted a real-time quantitative polymerase chain reaction (RT-qPCR) to assess the relative gene expression of OCT4 and PGP9.5. The relative expression of the OCT4 gene was down-regulated in the adult testes compared to its expression during prepubertal and neonatal life. The relative expression of the PGP9.5 gene was up-regulated in the neonatal testes and down-regulated in the prepubertal and adult testes. The spermatogonia were round, oval-to-ellipsoidal cells lying over the basement membrane (BM) with a round-to-oval nucleus. Based on the immunoexpression of the putative SSC markers, OCT4 and PGP9.5, we concluded that the proportion of stem cells was highest during the neonatal stage, followed by the prepubertal and prenatal stages. This finding sheds light on the dynamics of spermatogonial stem cells in buffalo testes at different developmental stages, providing valuable insights into these cells’ regulation and potential applications. |
| format | Article |
| id | doaj-art-e8117b056e0f4d4f8595db95bf36339b |
| institution | DOAJ |
| issn | 1049-5398 1532-2378 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Animal Biotechnology |
| spelling | doaj-art-e8117b056e0f4d4f8595db95bf36339b2025-08-20T03:13:50ZengTaylor & Francis GroupAnimal Biotechnology1049-53981532-23782024-12-0135110.1080/10495398.2023.2285509Analysis of OCT4 and PGP9.5 gene expression in prenatal and postnatal buffalo (Bubalus bubalis) testesAshritha Q. Carlo0Devendra Pathak1Ratan K. Choudhary2Opinder Singh3Neelam Bansal4Department of Veterinary Anatomy, College of Veterinary Sciences, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, IndiaDepartment of Veterinary Anatomy, College of Veterinary Sciences, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, IndiaAnimal Stem Cells Lab, College of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, IndiaDepartment of Veterinary Anatomy, College of Veterinary Sciences, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, IndiaDepartment of Veterinary Anatomy, College of Veterinary Sciences, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, IndiaThis study aimed to investigate and characterize the spermatogonial stem cells (SSCs) in buffaloes at different stages of development, including prenatal, neonatal, prepubertal, and adult testes. We sought a comprehensive understanding of these cells through a combination of histological, immunohistochemical, and ultrastructural analyses. Specifically, we examined changes in the expression of two potential SSC markers, OCT4 and PGP9.5, using immunohistochemistry. Additionally, we conducted a real-time quantitative polymerase chain reaction (RT-qPCR) to assess the relative gene expression of OCT4 and PGP9.5. The relative expression of the OCT4 gene was down-regulated in the adult testes compared to its expression during prepubertal and neonatal life. The relative expression of the PGP9.5 gene was up-regulated in the neonatal testes and down-regulated in the prepubertal and adult testes. The spermatogonia were round, oval-to-ellipsoidal cells lying over the basement membrane (BM) with a round-to-oval nucleus. Based on the immunoexpression of the putative SSC markers, OCT4 and PGP9.5, we concluded that the proportion of stem cells was highest during the neonatal stage, followed by the prepubertal and prenatal stages. This finding sheds light on the dynamics of spermatogonial stem cells in buffalo testes at different developmental stages, providing valuable insights into these cells’ regulation and potential applications.https://www.tandfonline.com/doi/10.1080/10495398.2023.2285509BuffaloSpermatogonial stem cellstestesOCT4PGP9.5 |
| spellingShingle | Ashritha Q. Carlo Devendra Pathak Ratan K. Choudhary Opinder Singh Neelam Bansal Analysis of OCT4 and PGP9.5 gene expression in prenatal and postnatal buffalo (Bubalus bubalis) testes Animal Biotechnology Buffalo Spermatogonial stem cells testes OCT4 PGP9.5 |
| title | Analysis of OCT4 and PGP9.5 gene expression in prenatal and postnatal buffalo (Bubalus bubalis) testes |
| title_full | Analysis of OCT4 and PGP9.5 gene expression in prenatal and postnatal buffalo (Bubalus bubalis) testes |
| title_fullStr | Analysis of OCT4 and PGP9.5 gene expression in prenatal and postnatal buffalo (Bubalus bubalis) testes |
| title_full_unstemmed | Analysis of OCT4 and PGP9.5 gene expression in prenatal and postnatal buffalo (Bubalus bubalis) testes |
| title_short | Analysis of OCT4 and PGP9.5 gene expression in prenatal and postnatal buffalo (Bubalus bubalis) testes |
| title_sort | analysis of oct4 and pgp9 5 gene expression in prenatal and postnatal buffalo bubalus bubalis testes |
| topic | Buffalo Spermatogonial stem cells testes OCT4 PGP9.5 |
| url | https://www.tandfonline.com/doi/10.1080/10495398.2023.2285509 |
| work_keys_str_mv | AT ashrithaqcarlo analysisofoct4andpgp95geneexpressioninprenatalandpostnatalbuffalobubalusbubalistestes AT devendrapathak analysisofoct4andpgp95geneexpressioninprenatalandpostnatalbuffalobubalusbubalistestes AT ratankchoudhary analysisofoct4andpgp95geneexpressioninprenatalandpostnatalbuffalobubalusbubalistestes AT opindersingh analysisofoct4andpgp95geneexpressioninprenatalandpostnatalbuffalobubalusbubalistestes AT neelambansal analysisofoct4andpgp95geneexpressioninprenatalandpostnatalbuffalobubalusbubalistestes |