Effects of macrolide antibiotics on blood inflammatory mediators and organ damage markers in lipopolysaccharide-induced pulmonary damage rats
<b>Aim:</b> The aim of this study was to determine the effect of macrolide antibiotics using in veterinary area on serum/ plasma inflammatory mediators and organ damage markers in lipopolysaccharide-induced pulmonary damage rats.<p> <b>Materials and Methods:</b> Total 1...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
|
| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=552 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | <b>Aim:</b> The aim of this study was to determine the effect of
macrolide antibiotics using in veterinary area on serum/
plasma inflammatory mediators and organ damage markers
in lipopolysaccharide-induced pulmonary damage rats.<p>
<b>Materials and Methods:</b> Total 198 Sprague-Dawley rats
were used. Six rats were used for a 0th sampling point for
all groups. 192 rats were divided into four equal groups as
lipopolysaccharide (Escherichia coli 0111:B4, 0.5 mg/200
μl, intratracheal), lipopolysaccharide + tylosin (10 mg/kg,
subcutaneously), lipopolysaccharide + tilmicosin (20 mg/
kg, subcutaneously) and lipopolysaccharide + tulathromycin
(2.5 mg/kg, subcutaneously). After the treatments,
blood samples were collected by cardiac puncture under
anesthesia at 0.5, 1, 1.5, 2, 4, 6, 12 and 24 hours. Serum
tumor necrosis factor α, interleukin-1β, interleukin-6, interleukin-
10 levels, and plasma 13, 14-dihydro-15-ketoprostaglandin
F<sub>2α</sub> levels were determined by ELISA, while
serum C-reactive protein, creatine kinase-MB, aspartate
aminotransferase, alanine aminotransferase, alkaline phosphatase,
cholesterol, triglyceride, blood urea nitrogen and
creatinine levels were determined by otoanalyzer. Area
under the concentration time curve (AUC<sub>0-24</sub>), maximal
concentration in plasma or serum (C<sub>max</sub>) and time to reach
Cmax (t<sub>max</sub>) values were determined by pharmacokinetic
computer program.<p>
<b>Results:</b> Macrolide antibiotics increased serum tumor
necrosis factor α and plasma 13, 14-dihydro-15-keto-prostaglandin
F<sub>2α</sub> levels.<p>
<b>Conclusion:</b> Macrolide antibiotics used in veterinary area
may no shown depression on immune system. Hovewer, investigation
is need that especially dose-depended manner
effects of macrolide antibiotics on immunosystem. |
|---|---|
| ISSN: | 1309-6958 2146-1953 |