Antioxidant Activity and Volatile and Phenolic Profiles of Essential Oil and Different Extracts of Wild Mint (Mentha longifolia) from the Pakistani Flora

The antioxidant activity and free radical scavenging capacity of the essential oil and three different extracts of wildly grown Mentha longifolia (M. longifolia) were studied. The essential oil from M. longifolia aerial parts was isolated by hydrodistillation technique using Clevenger-type apparatus...

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Main Authors: Tahseen Iqbal, Abdullah Ijaz Hussain, Shahzad Ali Shahid Chatha, Syed Ali Raza Naqvi, Tanveer Hussain Bokhari
Format: Article
Language:English
Published: Wiley 2013-01-01
Series:Journal of Analytical Methods in Chemistry
Online Access:http://dx.doi.org/10.1155/2013/536490
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author Tahseen Iqbal
Abdullah Ijaz Hussain
Shahzad Ali Shahid Chatha
Syed Ali Raza Naqvi
Tanveer Hussain Bokhari
author_facet Tahseen Iqbal
Abdullah Ijaz Hussain
Shahzad Ali Shahid Chatha
Syed Ali Raza Naqvi
Tanveer Hussain Bokhari
author_sort Tahseen Iqbal
collection DOAJ
description The antioxidant activity and free radical scavenging capacity of the essential oil and three different extracts of wildly grown Mentha longifolia (M. longifolia) were studied. The essential oil from M. longifolia aerial parts was isolated by hydrodistillation technique using Clevenger-type apparatus. The extracts were prepared with three solvents of different polarity (n-hexane, dichloromethane, and methanol) using Soxhlet extractor. Maximum extract yield was obtained with methanol (12.6 g/100 g) while the minimum with dichloromethane (3.50 g/100 g). The essential oil content was found to be 1.07 g/100 g. A total of 19 constituents were identified in the M. longifolia oil using GC/MS. The main components detected were piperitenone oxide, piperitenone, germacrene D, borneol, and β-caryophyllene. The total phenolics (TP) and total flavonoids (TF) contents of the methanol extract of M. longifolia were found to be significantly higher than dichloromethane and hexane extracts. The dichloromethane and methanol extracts exhibited excellent antioxidant activity as assessed by 2,2′-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging ability, bleaching β-carotene, and inhibition of linoleic acid peroxidation assays. The essential oil and hexane extract showed comparatively weaker antioxidant and free radical scavenging activities. The results of the study have validated the medicinal and antioxidant potential of M. longifolia essential oil and extracts.
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spelling doaj-art-e803b4a8a7224bc8a9de408003eb927f2025-08-20T03:21:03ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732013-01-01201310.1155/2013/536490536490Antioxidant Activity and Volatile and Phenolic Profiles of Essential Oil and Different Extracts of Wild Mint (Mentha longifolia) from the Pakistani FloraTahseen Iqbal0Abdullah Ijaz Hussain1Shahzad Ali Shahid Chatha2Syed Ali Raza Naqvi3Tanveer Hussain Bokhari4Institute of Chemistry, Government College University Faisalabad, Faisalabad 38000, PakistanInstitute of Chemistry, Government College University Faisalabad, Faisalabad 38000, PakistanInstitute of Chemistry, Government College University Faisalabad, Faisalabad 38000, PakistanInstitute of Chemistry, Government College University Faisalabad, Faisalabad 38000, PakistanInstitute of Chemistry, Government College University Faisalabad, Faisalabad 38000, PakistanThe antioxidant activity and free radical scavenging capacity of the essential oil and three different extracts of wildly grown Mentha longifolia (M. longifolia) were studied. The essential oil from M. longifolia aerial parts was isolated by hydrodistillation technique using Clevenger-type apparatus. The extracts were prepared with three solvents of different polarity (n-hexane, dichloromethane, and methanol) using Soxhlet extractor. Maximum extract yield was obtained with methanol (12.6 g/100 g) while the minimum with dichloromethane (3.50 g/100 g). The essential oil content was found to be 1.07 g/100 g. A total of 19 constituents were identified in the M. longifolia oil using GC/MS. The main components detected were piperitenone oxide, piperitenone, germacrene D, borneol, and β-caryophyllene. The total phenolics (TP) and total flavonoids (TF) contents of the methanol extract of M. longifolia were found to be significantly higher than dichloromethane and hexane extracts. The dichloromethane and methanol extracts exhibited excellent antioxidant activity as assessed by 2,2′-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging ability, bleaching β-carotene, and inhibition of linoleic acid peroxidation assays. The essential oil and hexane extract showed comparatively weaker antioxidant and free radical scavenging activities. The results of the study have validated the medicinal and antioxidant potential of M. longifolia essential oil and extracts.http://dx.doi.org/10.1155/2013/536490
spellingShingle Tahseen Iqbal
Abdullah Ijaz Hussain
Shahzad Ali Shahid Chatha
Syed Ali Raza Naqvi
Tanveer Hussain Bokhari
Antioxidant Activity and Volatile and Phenolic Profiles of Essential Oil and Different Extracts of Wild Mint (Mentha longifolia) from the Pakistani Flora
Journal of Analytical Methods in Chemistry
title Antioxidant Activity and Volatile and Phenolic Profiles of Essential Oil and Different Extracts of Wild Mint (Mentha longifolia) from the Pakistani Flora
title_full Antioxidant Activity and Volatile and Phenolic Profiles of Essential Oil and Different Extracts of Wild Mint (Mentha longifolia) from the Pakistani Flora
title_fullStr Antioxidant Activity and Volatile and Phenolic Profiles of Essential Oil and Different Extracts of Wild Mint (Mentha longifolia) from the Pakistani Flora
title_full_unstemmed Antioxidant Activity and Volatile and Phenolic Profiles of Essential Oil and Different Extracts of Wild Mint (Mentha longifolia) from the Pakistani Flora
title_short Antioxidant Activity and Volatile and Phenolic Profiles of Essential Oil and Different Extracts of Wild Mint (Mentha longifolia) from the Pakistani Flora
title_sort antioxidant activity and volatile and phenolic profiles of essential oil and different extracts of wild mint mentha longifolia from the pakistani flora
url http://dx.doi.org/10.1155/2013/536490
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