The streamlined genome of Phytomonas spp. relative to human pathogenic kinetoplastids reveals a parasite tailored for plants.
Members of the family Trypanosomatidae infect many organisms, including animals, plants and humans. Plant-infecting trypanosomes are grouped under the single genus Phytomonas, failing to reflect the wide biological and pathological diversity of these protists. While some Phytomonas spp. multiply in...
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Public Library of Science (PLoS)
2014-02-01
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| Series: | PLoS Genetics |
| Online Access: | https://doi.org/10.1371/journal.pgen.1004007 |
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| author | Betina M Porcel France Denoeud Fred Opperdoes Benjamin Noel Mohammed-Amine Madoui Tansy C Hammarton Mark C Field Corinne Da Silva Arnaud Couloux Julie Poulain Michael Katinka Kamel Jabbari Jean-Marc Aury David A Campbell Roxana Cintron Nicholas J Dickens Roberto Docampo Nancy R Sturm V Lila Koumandou Sandrine Fabre Pavel Flegontov Julius Lukeš Shulamit Michaeli Jeremy C Mottram Balázs Szöőr Dan Zilberstein Frédéric Bringaud Patrick Wincker Michel Dollet |
| author_facet | Betina M Porcel France Denoeud Fred Opperdoes Benjamin Noel Mohammed-Amine Madoui Tansy C Hammarton Mark C Field Corinne Da Silva Arnaud Couloux Julie Poulain Michael Katinka Kamel Jabbari Jean-Marc Aury David A Campbell Roxana Cintron Nicholas J Dickens Roberto Docampo Nancy R Sturm V Lila Koumandou Sandrine Fabre Pavel Flegontov Julius Lukeš Shulamit Michaeli Jeremy C Mottram Balázs Szöőr Dan Zilberstein Frédéric Bringaud Patrick Wincker Michel Dollet |
| author_sort | Betina M Porcel |
| collection | DOAJ |
| description | Members of the family Trypanosomatidae infect many organisms, including animals, plants and humans. Plant-infecting trypanosomes are grouped under the single genus Phytomonas, failing to reflect the wide biological and pathological diversity of these protists. While some Phytomonas spp. multiply in the latex of plants, or in fruit or seeds without apparent pathogenicity, others colonize the phloem sap and afflict plants of substantial economic value, including the coffee tree, coconut and oil palms. Plant trypanosomes have not been studied extensively at the genome level, a major gap in understanding and controlling pathogenesis. We describe the genome sequences of two plant trypanosomatids, one pathogenic isolate from a Guianan coconut and one non-symptomatic isolate from Euphorbia collected in France. Although these parasites have extremely distinct pathogenic impacts, very few genes are unique to either, with the vast majority of genes shared by both isolates. Significantly, both Phytomonas spp. genomes consist essentially of single copy genes for the bulk of their metabolic enzymes, whereas other trypanosomatids e.g. Leishmania and Trypanosoma possess multiple paralogous genes or families. Indeed, comparison with other trypanosomatid genomes revealed a highly streamlined genome, encoding for a minimized metabolic system while conserving the major pathways, and with retention of a full complement of endomembrane organelles, but with no evidence for functional complexity. Identification of the metabolic genes of Phytomonas provides opportunities for establishing in vitro culturing of these fastidious parasites and new tools for the control of agricultural plant disease. |
| format | Article |
| id | doaj-art-e7fb0e3eb1d140d592dc98e5882869be |
| institution | Kabale University |
| issn | 1553-7390 1553-7404 |
| language | English |
| publishDate | 2014-02-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj-art-e7fb0e3eb1d140d592dc98e5882869be2025-08-20T03:46:43ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042014-02-01102e100400710.1371/journal.pgen.1004007The streamlined genome of Phytomonas spp. relative to human pathogenic kinetoplastids reveals a parasite tailored for plants.Betina M PorcelFrance DenoeudFred OpperdoesBenjamin NoelMohammed-Amine MadouiTansy C HammartonMark C FieldCorinne Da SilvaArnaud CoulouxJulie PoulainMichael KatinkaKamel JabbariJean-Marc AuryDavid A CampbellRoxana CintronNicholas J DickensRoberto DocampoNancy R SturmV Lila KoumandouSandrine FabrePavel FlegontovJulius LukešShulamit MichaeliJeremy C MottramBalázs SzöőrDan ZilbersteinFrédéric BringaudPatrick WinckerMichel DolletMembers of the family Trypanosomatidae infect many organisms, including animals, plants and humans. Plant-infecting trypanosomes are grouped under the single genus Phytomonas, failing to reflect the wide biological and pathological diversity of these protists. While some Phytomonas spp. multiply in the latex of plants, or in fruit or seeds without apparent pathogenicity, others colonize the phloem sap and afflict plants of substantial economic value, including the coffee tree, coconut and oil palms. Plant trypanosomes have not been studied extensively at the genome level, a major gap in understanding and controlling pathogenesis. We describe the genome sequences of two plant trypanosomatids, one pathogenic isolate from a Guianan coconut and one non-symptomatic isolate from Euphorbia collected in France. Although these parasites have extremely distinct pathogenic impacts, very few genes are unique to either, with the vast majority of genes shared by both isolates. Significantly, both Phytomonas spp. genomes consist essentially of single copy genes for the bulk of their metabolic enzymes, whereas other trypanosomatids e.g. Leishmania and Trypanosoma possess multiple paralogous genes or families. Indeed, comparison with other trypanosomatid genomes revealed a highly streamlined genome, encoding for a minimized metabolic system while conserving the major pathways, and with retention of a full complement of endomembrane organelles, but with no evidence for functional complexity. Identification of the metabolic genes of Phytomonas provides opportunities for establishing in vitro culturing of these fastidious parasites and new tools for the control of agricultural plant disease.https://doi.org/10.1371/journal.pgen.1004007 |
| spellingShingle | Betina M Porcel France Denoeud Fred Opperdoes Benjamin Noel Mohammed-Amine Madoui Tansy C Hammarton Mark C Field Corinne Da Silva Arnaud Couloux Julie Poulain Michael Katinka Kamel Jabbari Jean-Marc Aury David A Campbell Roxana Cintron Nicholas J Dickens Roberto Docampo Nancy R Sturm V Lila Koumandou Sandrine Fabre Pavel Flegontov Julius Lukeš Shulamit Michaeli Jeremy C Mottram Balázs Szöőr Dan Zilberstein Frédéric Bringaud Patrick Wincker Michel Dollet The streamlined genome of Phytomonas spp. relative to human pathogenic kinetoplastids reveals a parasite tailored for plants. PLoS Genetics |
| title | The streamlined genome of Phytomonas spp. relative to human pathogenic kinetoplastids reveals a parasite tailored for plants. |
| title_full | The streamlined genome of Phytomonas spp. relative to human pathogenic kinetoplastids reveals a parasite tailored for plants. |
| title_fullStr | The streamlined genome of Phytomonas spp. relative to human pathogenic kinetoplastids reveals a parasite tailored for plants. |
| title_full_unstemmed | The streamlined genome of Phytomonas spp. relative to human pathogenic kinetoplastids reveals a parasite tailored for plants. |
| title_short | The streamlined genome of Phytomonas spp. relative to human pathogenic kinetoplastids reveals a parasite tailored for plants. |
| title_sort | streamlined genome of phytomonas spp relative to human pathogenic kinetoplastids reveals a parasite tailored for plants |
| url | https://doi.org/10.1371/journal.pgen.1004007 |
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