Entrapment of protease from Bacillus sp. in polyvinyl alcohol hydrogels
This study highlights the effective immobilization of protease from Bacillus sp. in polyvinyl alcohol hydrogels and its characterization. Both free and entrapped proteases exhibited optimal activity at pH 8.0 and 55°C, indicating that the immobilization did not significantly alter the enzyme's...
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| Format: | Article |
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Tunç ÇATAL
2025-01-01
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| Series: | The European Chemistry and Biotechnology Journal |
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| Online Access: | https://euchembioj.com/index.php/pub/article/view/38 |
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| author | Fatih Mehmet Hasbek Deniz Yildirim |
| author_facet | Fatih Mehmet Hasbek Deniz Yildirim |
| author_sort | Fatih Mehmet Hasbek |
| collection | DOAJ |
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This study highlights the effective immobilization of protease from Bacillus sp. in polyvinyl alcohol hydrogels and its characterization. Both free and entrapped proteases exhibited optimal activity at pH 8.0 and 55°C, indicating that the immobilization did not significantly alter the enzyme's fundamental properties. The entrapment in polyvinyl alcohol hydrogels significantly enhanced thermal stability. After 24 hours at 55°C, the free protease retained only 19% of its initial activity, whereas the entrapped protease retained 72%. The entrapped protease showed a longer half-life of 53.3 hours compared to 10.6 hours for the free protease. The Km and Vmax values of free protease were determined to be 0.5 mg/mL and 23.3 U/mg protein, respectively, for casein. These values were found to be 0.2 mg/mL and 23.8 U/mg protein, respectively for the entrapped protease. The entrapped protease retained 58% of its initial activity after 5 reuses in a batch reactor. As a result, the entrapment of Bacillus sp. protease in polyvinyl alcohol is an effective immobilization method due to its simplicity, low cost, and ability to provide a 5-fold increase in thermal stability.
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| format | Article |
| id | doaj-art-e7f7c7f7ec2545a08b3ba557ed6e4db3 |
| institution | OA Journals |
| issn | 3023-5839 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Tunç ÇATAL |
| record_format | Article |
| series | The European Chemistry and Biotechnology Journal |
| spelling | doaj-art-e7f7c7f7ec2545a08b3ba557ed6e4db32025-08-20T02:29:11ZengTunç ÇATALThe European Chemistry and Biotechnology Journal3023-58392025-01-013Entrapment of protease from Bacillus sp. in polyvinyl alcohol hydrogelsFatih Mehmet Hasbek0https://orcid.org/0009-0000-4780-9745Deniz Yildirim1https://orcid.org/0000-0002-5041-8160Cukurova University Cukurova University This study highlights the effective immobilization of protease from Bacillus sp. in polyvinyl alcohol hydrogels and its characterization. Both free and entrapped proteases exhibited optimal activity at pH 8.0 and 55°C, indicating that the immobilization did not significantly alter the enzyme's fundamental properties. The entrapment in polyvinyl alcohol hydrogels significantly enhanced thermal stability. After 24 hours at 55°C, the free protease retained only 19% of its initial activity, whereas the entrapped protease retained 72%. The entrapped protease showed a longer half-life of 53.3 hours compared to 10.6 hours for the free protease. The Km and Vmax values of free protease were determined to be 0.5 mg/mL and 23.3 U/mg protein, respectively, for casein. These values were found to be 0.2 mg/mL and 23.8 U/mg protein, respectively for the entrapped protease. The entrapped protease retained 58% of its initial activity after 5 reuses in a batch reactor. As a result, the entrapment of Bacillus sp. protease in polyvinyl alcohol is an effective immobilization method due to its simplicity, low cost, and ability to provide a 5-fold increase in thermal stability. https://euchembioj.com/index.php/pub/article/view/38Bacillus sp.immobilizationpolyvinyl alcoholprotease |
| spellingShingle | Fatih Mehmet Hasbek Deniz Yildirim Entrapment of protease from Bacillus sp. in polyvinyl alcohol hydrogels The European Chemistry and Biotechnology Journal Bacillus sp. immobilization polyvinyl alcohol protease |
| title | Entrapment of protease from Bacillus sp. in polyvinyl alcohol hydrogels |
| title_full | Entrapment of protease from Bacillus sp. in polyvinyl alcohol hydrogels |
| title_fullStr | Entrapment of protease from Bacillus sp. in polyvinyl alcohol hydrogels |
| title_full_unstemmed | Entrapment of protease from Bacillus sp. in polyvinyl alcohol hydrogels |
| title_short | Entrapment of protease from Bacillus sp. in polyvinyl alcohol hydrogels |
| title_sort | entrapment of protease from bacillus sp in polyvinyl alcohol hydrogels |
| topic | Bacillus sp. immobilization polyvinyl alcohol protease |
| url | https://euchembioj.com/index.php/pub/article/view/38 |
| work_keys_str_mv | AT fatihmehmethasbek entrapmentofproteasefrombacillusspinpolyvinylalcoholhydrogels AT denizyildirim entrapmentofproteasefrombacillusspinpolyvinylalcoholhydrogels |