Genetic characterization and transmission of the multidrug resistance gene cfr in fecal and environmental pathways on a chicken farm in China
The emergence and spread of the multidrug-resistant gene cfr have raised significant public health concerns worldwide. To investigate its prevalence and dissemination dynamics, 18 cfr-positive strains were isolated in 2021 from fecal and environmental samples. Antimicrobial susceptibility testing sh...
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Elsevier
2025-06-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579125003189 |
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| author | Yiming Feng Tiantian Li Shiyun Zhao Xuexue Li Yajun Zhai Li Yuan Jianhua Liu Gongzheng Hu Dandan He Yushan Pan |
| author_facet | Yiming Feng Tiantian Li Shiyun Zhao Xuexue Li Yajun Zhai Li Yuan Jianhua Liu Gongzheng Hu Dandan He Yushan Pan |
| author_sort | Yiming Feng |
| collection | DOAJ |
| description | The emergence and spread of the multidrug-resistant gene cfr have raised significant public health concerns worldwide. To investigate its prevalence and dissemination dynamics, 18 cfr-positive strains were isolated in 2021 from fecal and environmental samples. Antimicrobial susceptibility testing showed that all strains were 100 % multidrug-resistant. Conjugation experiments demonstrated that a cfr- carrying IncFII(K)-IncR-IncFIB multi-replicon plasmid could transfer to E. coli J53. S1-nuclease digestion and Southern blotting identified cfr on plasmids of varying sizes, while whole-genome sequencing confirmed its presence on multiple plasmid types: IncX4, IncN, IncFII(K)-IncR-IncFIB, IncFIB-IncFII-IncR-IncHI2-IncHI2A multi-replicon plasmids, and two plasmids of unknown types. Genetic environment analysis revealed that cfr is categorized into five distinct structures (Types I-V). Reverse PCR results showed that Types I, II, and IV can form three circular intermediates of varying lengths (cfr-IS26). Network analysis further indicated strong association between cfr, tet(M), and dfrA14 mediated by IS26. Phylogenetic analysis revealed that the four ST1140 E. coli strains and all nine K. pneumoniae strains showed minimal genetic divergence. These findings suggest both clonal and horizontal transmission of cfr within the poultry farm. Continuous monitoring of cfr in animal-related environments is essential to mitigate its potential transfer to humans. |
| format | Article |
| id | doaj-art-e7e95678050d402cb07a11801be9ebc0 |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-e7e95678050d402cb07a11801be9ebc02025-08-20T03:24:44ZengElsevierPoultry Science0032-57912025-06-01104610507910.1016/j.psj.2025.105079Genetic characterization and transmission of the multidrug resistance gene cfr in fecal and environmental pathways on a chicken farm in ChinaYiming Feng0Tiantian Li1Shiyun Zhao2Xuexue Li3Yajun Zhai4Li Yuan5Jianhua Liu6Gongzheng Hu7Dandan He8Yushan Pan9College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, PR ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou, PR ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou, PR ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou, PR ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou, PR China; Ministry of Education Key Laboratory for Animal Pathogens and Biosafety, Zhengzhou, Henan Province, PR China; Henan Province Key Laboratory of Animal Food Pathogens Surveillance, PR ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou, PR China; Ministry of Education Key Laboratory for Animal Pathogens and Biosafety, Zhengzhou, Henan Province, PR China; Henan Province Key Laboratory of Animal Food Pathogens Surveillance, PR ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou, PR ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou, PR China; Ministry of Education Key Laboratory for Animal Pathogens and Biosafety, Zhengzhou, Henan Province, PR China; Henan Province Key Laboratory of Animal Food Pathogens Surveillance, PR ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou, PR China; Ministry of Education Key Laboratory for Animal Pathogens and Biosafety, Zhengzhou, Henan Province, PR China; Henan Province Key Laboratory of Animal Food Pathogens Surveillance, PR China; Correspondence authors.College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, PR China; Correspondence authors.The emergence and spread of the multidrug-resistant gene cfr have raised significant public health concerns worldwide. To investigate its prevalence and dissemination dynamics, 18 cfr-positive strains were isolated in 2021 from fecal and environmental samples. Antimicrobial susceptibility testing showed that all strains were 100 % multidrug-resistant. Conjugation experiments demonstrated that a cfr- carrying IncFII(K)-IncR-IncFIB multi-replicon plasmid could transfer to E. coli J53. S1-nuclease digestion and Southern blotting identified cfr on plasmids of varying sizes, while whole-genome sequencing confirmed its presence on multiple plasmid types: IncX4, IncN, IncFII(K)-IncR-IncFIB, IncFIB-IncFII-IncR-IncHI2-IncHI2A multi-replicon plasmids, and two plasmids of unknown types. Genetic environment analysis revealed that cfr is categorized into five distinct structures (Types I-V). Reverse PCR results showed that Types I, II, and IV can form three circular intermediates of varying lengths (cfr-IS26). Network analysis further indicated strong association between cfr, tet(M), and dfrA14 mediated by IS26. Phylogenetic analysis revealed that the four ST1140 E. coli strains and all nine K. pneumoniae strains showed minimal genetic divergence. These findings suggest both clonal and horizontal transmission of cfr within the poultry farm. Continuous monitoring of cfr in animal-related environments is essential to mitigate its potential transfer to humans.http://www.sciencedirect.com/science/article/pii/S0032579125003189ChickenFarm environmentcfrTransmissionPlasmid |
| spellingShingle | Yiming Feng Tiantian Li Shiyun Zhao Xuexue Li Yajun Zhai Li Yuan Jianhua Liu Gongzheng Hu Dandan He Yushan Pan Genetic characterization and transmission of the multidrug resistance gene cfr in fecal and environmental pathways on a chicken farm in China Poultry Science Chicken Farm environment cfr Transmission Plasmid |
| title | Genetic characterization and transmission of the multidrug resistance gene cfr in fecal and environmental pathways on a chicken farm in China |
| title_full | Genetic characterization and transmission of the multidrug resistance gene cfr in fecal and environmental pathways on a chicken farm in China |
| title_fullStr | Genetic characterization and transmission of the multidrug resistance gene cfr in fecal and environmental pathways on a chicken farm in China |
| title_full_unstemmed | Genetic characterization and transmission of the multidrug resistance gene cfr in fecal and environmental pathways on a chicken farm in China |
| title_short | Genetic characterization and transmission of the multidrug resistance gene cfr in fecal and environmental pathways on a chicken farm in China |
| title_sort | genetic characterization and transmission of the multidrug resistance gene cfr in fecal and environmental pathways on a chicken farm in china |
| topic | Chicken Farm environment cfr Transmission Plasmid |
| url | http://www.sciencedirect.com/science/article/pii/S0032579125003189 |
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