The impact of multiple exposures and movement on the fear response of poultry
Fear tests are a common research method to assess the affective state of an animal. This study aimed to assess: 1) the impact of repeated exposure to fear tests on fear response and, 2) how the addition of movement to fear stimuli during a novel object test impacts bird fear response. Over two trial...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-01-01
|
| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124011726 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832591878613106688 |
|---|---|
| author | Alexandra Jackson Marcela Quino Anusha Gautam Melissa Gilpin Katie Still Denise Landers Bethany Baker-Cook |
| author_facet | Alexandra Jackson Marcela Quino Anusha Gautam Melissa Gilpin Katie Still Denise Landers Bethany Baker-Cook |
| author_sort | Alexandra Jackson |
| collection | DOAJ |
| description | Fear tests are a common research method to assess the affective state of an animal. This study aimed to assess: 1) the impact of repeated exposure to fear tests on fear response and, 2) how the addition of movement to fear stimuli during a novel object test impacts bird fear response. Over two trials, a total of 3,600 Ross 308 birds (1800 birds/trial) were raised until 42 days of age. At 23d, 30d, and 37d, three fear tests were performed, novel object, human approach, and response to observer tests. The novel object test was split into three movement treatments, stationary, intermittent, and continuous. The response to observer test was performed before and after the other tests. Data from the human approach test was analyzed for age effect by ANOVA. Novel object data was analyzed as repeated measures ANOVA for the effect of movement. Differences in response to observer were analyzed using a paired T-test. Repeated exposure to fear test within the same day decreased the fear response, with response to observer results before fear testing (66%) differing from after (42%, P<0.01). The fear response also decreased with multiple consecutive exposures. Both latency to human approach and latency to novel object interaction were longest on 23d, then 30d, and shortest on 37d (P<0.01). For both the human approach and the novel object test, at all-time points, the number of birds interested in the human or object was higher on 37d than 23d (P<0.01). The addition of movement to the novel object test increased the fear response at 23 days but decreased the fear response at 37 days. Overall, the repeated exposure of birds to fear tests reduced the bird's fear response, both for the repeated exposure to different fear tests on a single day and the repeated exposure to one type of fear test over the length of a flock. This impact of repeated exposure is important to recognize when designing experiments that utilize fear tests. |
| format | Article |
| id | doaj-art-e7e4eb2e7bd44ae693bfb2fe1a6fb978 |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-e7e4eb2e7bd44ae693bfb2fe1a6fb9782025-01-22T05:40:37ZengElsevierPoultry Science0032-57912025-01-011041104594The impact of multiple exposures and movement on the fear response of poultryAlexandra Jackson0Marcela Quino1Anusha Gautam2Melissa Gilpin3Katie Still4Denise Landers5Bethany Baker-Cook6Department of Poultry Science, Auburn University, Office 302E, 260 Lem Morrison Dr., Auburn, Auburn, AL 36849, USADepartment of Poultry Science, Auburn University, Office 302E, 260 Lem Morrison Dr., Auburn, Auburn, AL 36849, USADepartment of Poultry Science, Auburn University, Office 302E, 260 Lem Morrison Dr., Auburn, Auburn, AL 36849, USADepartment of Poultry Science, Auburn University, Office 302E, 260 Lem Morrison Dr., Auburn, Auburn, AL 36849, USADepartment of Poultry Science, Auburn University, Office 302E, 260 Lem Morrison Dr., Auburn, Auburn, AL 36849, USADepartment of Poultry Science, Auburn University, Office 302E, 260 Lem Morrison Dr., Auburn, Auburn, AL 36849, USACorresponding author.; Department of Poultry Science, Auburn University, Office 302E, 260 Lem Morrison Dr., Auburn, Auburn, AL 36849, USAFear tests are a common research method to assess the affective state of an animal. This study aimed to assess: 1) the impact of repeated exposure to fear tests on fear response and, 2) how the addition of movement to fear stimuli during a novel object test impacts bird fear response. Over two trials, a total of 3,600 Ross 308 birds (1800 birds/trial) were raised until 42 days of age. At 23d, 30d, and 37d, three fear tests were performed, novel object, human approach, and response to observer tests. The novel object test was split into three movement treatments, stationary, intermittent, and continuous. The response to observer test was performed before and after the other tests. Data from the human approach test was analyzed for age effect by ANOVA. Novel object data was analyzed as repeated measures ANOVA for the effect of movement. Differences in response to observer were analyzed using a paired T-test. Repeated exposure to fear test within the same day decreased the fear response, with response to observer results before fear testing (66%) differing from after (42%, P<0.01). The fear response also decreased with multiple consecutive exposures. Both latency to human approach and latency to novel object interaction were longest on 23d, then 30d, and shortest on 37d (P<0.01). For both the human approach and the novel object test, at all-time points, the number of birds interested in the human or object was higher on 37d than 23d (P<0.01). The addition of movement to the novel object test increased the fear response at 23 days but decreased the fear response at 37 days. Overall, the repeated exposure of birds to fear tests reduced the bird's fear response, both for the repeated exposure to different fear tests on a single day and the repeated exposure to one type of fear test over the length of a flock. This impact of repeated exposure is important to recognize when designing experiments that utilize fear tests.http://www.sciencedirect.com/science/article/pii/S0032579124011726Novel objectVoluntary human approachFearfulnessBroilersWelfare assessments |
| spellingShingle | Alexandra Jackson Marcela Quino Anusha Gautam Melissa Gilpin Katie Still Denise Landers Bethany Baker-Cook The impact of multiple exposures and movement on the fear response of poultry Poultry Science Novel object Voluntary human approach Fearfulness Broilers Welfare assessments |
| title | The impact of multiple exposures and movement on the fear response of poultry |
| title_full | The impact of multiple exposures and movement on the fear response of poultry |
| title_fullStr | The impact of multiple exposures and movement on the fear response of poultry |
| title_full_unstemmed | The impact of multiple exposures and movement on the fear response of poultry |
| title_short | The impact of multiple exposures and movement on the fear response of poultry |
| title_sort | impact of multiple exposures and movement on the fear response of poultry |
| topic | Novel object Voluntary human approach Fearfulness Broilers Welfare assessments |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124011726 |
| work_keys_str_mv | AT alexandrajackson theimpactofmultipleexposuresandmovementonthefearresponseofpoultry AT marcelaquino theimpactofmultipleexposuresandmovementonthefearresponseofpoultry AT anushagautam theimpactofmultipleexposuresandmovementonthefearresponseofpoultry AT melissagilpin theimpactofmultipleexposuresandmovementonthefearresponseofpoultry AT katiestill theimpactofmultipleexposuresandmovementonthefearresponseofpoultry AT deniselanders theimpactofmultipleexposuresandmovementonthefearresponseofpoultry AT bethanybakercook theimpactofmultipleexposuresandmovementonthefearresponseofpoultry AT alexandrajackson impactofmultipleexposuresandmovementonthefearresponseofpoultry AT marcelaquino impactofmultipleexposuresandmovementonthefearresponseofpoultry AT anushagautam impactofmultipleexposuresandmovementonthefearresponseofpoultry AT melissagilpin impactofmultipleexposuresandmovementonthefearresponseofpoultry AT katiestill impactofmultipleexposuresandmovementonthefearresponseofpoultry AT deniselanders impactofmultipleexposuresandmovementonthefearresponseofpoultry AT bethanybakercook impactofmultipleexposuresandmovementonthefearresponseofpoultry |