Zymosan-induced luminol-dependent chemiluminescence response of circulating and extravasated leukocytes in experimental sepsis
This study examines a concurrent profiling of circulating and extravasated polymorphonuclear leukocytes (PMNs) in a rat model of experimental sepsis. Fecal peritonitis was induced in Wistar male rats by intraperitoneal instillation of a fecal suspension in saline (1:1 w/v). Blood and peritoneal flui...
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| Format: | Article |
| Language: | English |
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Wiley
2004-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1080/09629350410001688558 |
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| author | Haseeb Ahmad Khan |
| author_facet | Haseeb Ahmad Khan |
| author_sort | Haseeb Ahmad Khan |
| collection | DOAJ |
| description | This study examines a concurrent profiling of circulating and extravasated polymorphonuclear leukocytes (PMNs) in a rat model of experimental sepsis. Fecal peritonitis was induced in Wistar male rats by intraperitoneal instillation of a fecal suspension in saline (1:1 w/v). Blood and peritoneal fluid were collected 8 h following fecal inoculation for the evaluation of inflammatory response of PMNs using zymosan-induced luminol-dependent chemiluminescence. Fifty microliters of pre-diluted blood or peritoneal fluid samples were mixed with 150 μl of reaction mixture (4×10−4 M luminol+50 μg opsonized zymosan+0.1% gelatin in Hank's balanced salt solution) and the chemiluminescence signal was measured in a luminometer at 37˚C. Fecal peritonitis caused a significant leukocytopenia (3540±297 mm−3 versus control value of 7525±711 mm−3, p<0.001) accompanied by massive infiltration of PMNs in the peritoneal cavity (34700±4006 versus 7325±425 mm−3, p<0.001). The phagocytic activity of circulating blood PMNs was down-regulated whereas a significant up-regulation was observed in the activity of PMNs from peritoneal fluid. In conclusion, this study clearly demonstrates sepsis-induced alterations in both blood and peritoneal fluid PMNs and their quantitative assessment may be helpful in disease evaluation and designing effective therapies. |
| format | Article |
| id | doaj-art-e7e00353c034479aabb024765d8d06cc |
| institution | DOAJ |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2004-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-e7e00353c034479aabb024765d8d06cc2025-08-20T03:21:02ZengWileyMediators of Inflammation0962-93511466-18612004-01-0113212312510.1080/09629350410001688558Zymosan-induced luminol-dependent chemiluminescence response of circulating and extravasated leukocytes in experimental sepsisHaseeb Ahmad Khan0Research Center, Riyadh Al Kharj Hospital Program, P.O. Box 7897 (T-835), Riyadh 11159, Saudi ArabiaThis study examines a concurrent profiling of circulating and extravasated polymorphonuclear leukocytes (PMNs) in a rat model of experimental sepsis. Fecal peritonitis was induced in Wistar male rats by intraperitoneal instillation of a fecal suspension in saline (1:1 w/v). Blood and peritoneal fluid were collected 8 h following fecal inoculation for the evaluation of inflammatory response of PMNs using zymosan-induced luminol-dependent chemiluminescence. Fifty microliters of pre-diluted blood or peritoneal fluid samples were mixed with 150 μl of reaction mixture (4×10−4 M luminol+50 μg opsonized zymosan+0.1% gelatin in Hank's balanced salt solution) and the chemiluminescence signal was measured in a luminometer at 37˚C. Fecal peritonitis caused a significant leukocytopenia (3540±297 mm−3 versus control value of 7525±711 mm−3, p<0.001) accompanied by massive infiltration of PMNs in the peritoneal cavity (34700±4006 versus 7325±425 mm−3, p<0.001). The phagocytic activity of circulating blood PMNs was down-regulated whereas a significant up-regulation was observed in the activity of PMNs from peritoneal fluid. In conclusion, this study clearly demonstrates sepsis-induced alterations in both blood and peritoneal fluid PMNs and their quantitative assessment may be helpful in disease evaluation and designing effective therapies.http://dx.doi.org/10.1080/09629350410001688558 |
| spellingShingle | Haseeb Ahmad Khan Zymosan-induced luminol-dependent chemiluminescence response of circulating and extravasated leukocytes in experimental sepsis Mediators of Inflammation |
| title | Zymosan-induced luminol-dependent chemiluminescence response of circulating and extravasated leukocytes in experimental sepsis |
| title_full | Zymosan-induced luminol-dependent chemiluminescence response of circulating and extravasated leukocytes in experimental sepsis |
| title_fullStr | Zymosan-induced luminol-dependent chemiluminescence response of circulating and extravasated leukocytes in experimental sepsis |
| title_full_unstemmed | Zymosan-induced luminol-dependent chemiluminescence response of circulating and extravasated leukocytes in experimental sepsis |
| title_short | Zymosan-induced luminol-dependent chemiluminescence response of circulating and extravasated leukocytes in experimental sepsis |
| title_sort | zymosan induced luminol dependent chemiluminescence response of circulating and extravasated leukocytes in experimental sepsis |
| url | http://dx.doi.org/10.1080/09629350410001688558 |
| work_keys_str_mv | AT haseebahmadkhan zymosaninducedluminoldependentchemiluminescenceresponseofcirculatingandextravasatedleukocytesinexperimentalsepsis |