Molecular characterization of Apple necrotic mosaic virus identified in crabapple (Malus spp.) tree of China
Apple necrotic mosaic virus (ApNMV) was identified in crabapple trees with mosaic symptoms from Zaozhuang, Shandong Province, China, by reverse transcription polymerase chain reaction (RT-PCR) analysis. The complete nucleotide sequences of one isolate from crabapple (ApNMV-Hai) and two isolates from...
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KeAi Communications Co., Ltd.
2019-03-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311918621161 |
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| author | Guo-jun HU Ya-feng DONG Zun-ping ZHANG Xu-dong FAN Fang REN |
| author_facet | Guo-jun HU Ya-feng DONG Zun-ping ZHANG Xu-dong FAN Fang REN |
| author_sort | Guo-jun HU |
| collection | DOAJ |
| description | Apple necrotic mosaic virus (ApNMV) was identified in crabapple trees with mosaic symptoms from Zaozhuang, Shandong Province, China, by reverse transcription polymerase chain reaction (RT-PCR) analysis. The complete nucleotide sequences of one isolate from crabapple (ApNMV-Hai) and two isolates from apple (ApNMV-Hua and -Qu) were determined. The sizes of genomic RNA1, 2 and 3 of the three isolates differed from those of the previously reported isolate ApNMV-P126 from Japanese apple, especially RNA3. Compared with the nucleotide (nt) sequence of RNA3 in isolate P126, those in the Hai and Qu isolates were 7 and 33 nt shorter, respectively, and that of isolate Hua was 7 nt longer. Alignment analyses showed that these differences in size were mainly due to differences in the lengths of the 5’ untranslated region (UTR) and the UTR region between the ORFs encoding the movement protein and the coat protein. In the phylogenetic trees constructed using the full genomic sequences of RNA1, 2 and 3, the isolate Hai clustered into a group with the isolate Qu in the RNA1 tree, but formed an individual branch in the RNA2 and 3 trees. Three recombination events were identified in the nucleotide sequences of RNA1 and 2 among the isolates ApNMV-Hai, -Hua, and -Qu. This is the first report of the full genome sequence of ApNMV in crabapple. |
| format | Article |
| id | doaj-art-e7dfdb03bf5a40e59fc231bdfb9900ef |
| institution | Kabale University |
| issn | 2095-3119 |
| language | English |
| publishDate | 2019-03-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-e7dfdb03bf5a40e59fc231bdfb9900ef2025-08-20T03:58:04ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192019-03-0118369870110.1016/S2095-3119(18)62116-1Molecular characterization of Apple necrotic mosaic virus identified in crabapple (Malus spp.) tree of ChinaGuo-jun HU0Ya-feng DONG1Zun-ping ZHANG2Xu-dong FAN3Fang REN4Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, P.R. ChinaCorrespondence DONG Ya-feng, Tel/Fax: +86-429-3598278; Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, P.R. ChinaResearch Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, P.R. ChinaResearch Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, P.R. ChinaResearch Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, P.R. ChinaApple necrotic mosaic virus (ApNMV) was identified in crabapple trees with mosaic symptoms from Zaozhuang, Shandong Province, China, by reverse transcription polymerase chain reaction (RT-PCR) analysis. The complete nucleotide sequences of one isolate from crabapple (ApNMV-Hai) and two isolates from apple (ApNMV-Hua and -Qu) were determined. The sizes of genomic RNA1, 2 and 3 of the three isolates differed from those of the previously reported isolate ApNMV-P126 from Japanese apple, especially RNA3. Compared with the nucleotide (nt) sequence of RNA3 in isolate P126, those in the Hai and Qu isolates were 7 and 33 nt shorter, respectively, and that of isolate Hua was 7 nt longer. Alignment analyses showed that these differences in size were mainly due to differences in the lengths of the 5’ untranslated region (UTR) and the UTR region between the ORFs encoding the movement protein and the coat protein. In the phylogenetic trees constructed using the full genomic sequences of RNA1, 2 and 3, the isolate Hai clustered into a group with the isolate Qu in the RNA1 tree, but formed an individual branch in the RNA2 and 3 trees. Three recombination events were identified in the nucleotide sequences of RNA1 and 2 among the isolates ApNMV-Hai, -Hua, and -Qu. This is the first report of the full genome sequence of ApNMV in crabapple.http://www.sciencedirect.com/science/article/pii/S2095311918621161crabapplemosaic symptomApple necrotic mosaic virusmolecular characterization |
| spellingShingle | Guo-jun HU Ya-feng DONG Zun-ping ZHANG Xu-dong FAN Fang REN Molecular characterization of Apple necrotic mosaic virus identified in crabapple (Malus spp.) tree of China Journal of Integrative Agriculture crabapple mosaic symptom Apple necrotic mosaic virus molecular characterization |
| title | Molecular characterization of Apple necrotic mosaic virus identified in crabapple (Malus spp.) tree of China |
| title_full | Molecular characterization of Apple necrotic mosaic virus identified in crabapple (Malus spp.) tree of China |
| title_fullStr | Molecular characterization of Apple necrotic mosaic virus identified in crabapple (Malus spp.) tree of China |
| title_full_unstemmed | Molecular characterization of Apple necrotic mosaic virus identified in crabapple (Malus spp.) tree of China |
| title_short | Molecular characterization of Apple necrotic mosaic virus identified in crabapple (Malus spp.) tree of China |
| title_sort | molecular characterization of apple necrotic mosaic virus identified in crabapple malus spp tree of china |
| topic | crabapple mosaic symptom Apple necrotic mosaic virus molecular characterization |
| url | http://www.sciencedirect.com/science/article/pii/S2095311918621161 |
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