Novel Bioceramic Urethral Bulking Agents Elicit Improved Host Tissue Responses in a Rat Model
Objectives. To test the physical properties and host response to the bioceramic particles, silica-calcium phosphate (SCPC10) and Cristobalite, in a rat animal model and compare their biocompatibility to the current clinically utilized urethral bulking materials. Material and Methods. The novel bulki...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Advances in Urology |
| Online Access: | http://dx.doi.org/10.1155/2016/1282531 |
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| author | Travis K. Mann-Gow Benjamin J. King Ahmed El-Ghannam Christine Knabe-Ducheyne Masatoshi Kida Ole M. Dall Jan Krhut Peter Zvara |
| author_facet | Travis K. Mann-Gow Benjamin J. King Ahmed El-Ghannam Christine Knabe-Ducheyne Masatoshi Kida Ole M. Dall Jan Krhut Peter Zvara |
| author_sort | Travis K. Mann-Gow |
| collection | DOAJ |
| description | Objectives. To test the physical properties and host response to the bioceramic particles, silica-calcium phosphate (SCPC10) and Cristobalite, in a rat animal model and compare their biocompatibility to the current clinically utilized urethral bulking materials. Material and Methods. The novel bulking materials, SCPC10 and Cristobalite, were suspended in hyaluronic acid sodium salt and injected into the mid urethra of a rat. Additional animals were injected with bulking materials currently in clinical use. Physiological response was assessed using voiding trials, and host tissue response was evaluated using hard tissue histology and immunohistochemical analysis. Distant organs were evaluated for the presence of particles or their components. Results. Histological analysis of the urethral tissue five months after injection showed that both SCPC10 and Cristobalite induced a more robust fibroblastic and histiocytic reaction, promoting integration and encapsulation of the particle aggregates, leading to a larger bulking effect. Concentrations of Ca, Na, Si, and P ions in the experimental groups were comparable to control animals. Conclusions. This side-by-side examination of urethral bulking agents using a rat animal model and hard tissue histology techniques compared two newly developed bioactive ceramic particles to three of the currently used bulking agents. The local host tissue response and bulking effects of bioceramic particles were superior while also possessing a comparable safety profile. |
| format | Article |
| id | doaj-art-e7de61f202fd4d8ba1669f74d339f698 |
| institution | Kabale University |
| issn | 1687-6369 1687-6377 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Urology |
| spelling | doaj-art-e7de61f202fd4d8ba1669f74d339f6982025-02-03T01:29:05ZengWileyAdvances in Urology1687-63691687-63772016-01-01201610.1155/2016/12825311282531Novel Bioceramic Urethral Bulking Agents Elicit Improved Host Tissue Responses in a Rat ModelTravis K. Mann-Gow0Benjamin J. King1Ahmed El-Ghannam2Christine Knabe-Ducheyne3Masatoshi Kida4Ole M. Dall5Jan Krhut6Peter Zvara7Department of Surgery, University of Vermont, Burlington, VT 05405, USADepartment of Surgery, University of Vermont, Burlington, VT 05405, USADepartment of Mechanical Engineering and Engineering Science, University of North Carolina at Charlotte, Charlotte, NC 28223, USADepartment of Medicine, Philipps University of Marburg, 35039 Marburg, GermanyDepartment of Pathology and Laboratory Medicine, University of Vermont, Burlington, VT 05405, USATAK Biopharma, 2000 Frederiksberg, DenmarkDepartment of Surgical Sciences, Ostrava University, 703 00 Ostrava, Czech RepublicDepartment of Surgery, University of Vermont, Burlington, VT 05405, USAObjectives. To test the physical properties and host response to the bioceramic particles, silica-calcium phosphate (SCPC10) and Cristobalite, in a rat animal model and compare their biocompatibility to the current clinically utilized urethral bulking materials. Material and Methods. The novel bulking materials, SCPC10 and Cristobalite, were suspended in hyaluronic acid sodium salt and injected into the mid urethra of a rat. Additional animals were injected with bulking materials currently in clinical use. Physiological response was assessed using voiding trials, and host tissue response was evaluated using hard tissue histology and immunohistochemical analysis. Distant organs were evaluated for the presence of particles or their components. Results. Histological analysis of the urethral tissue five months after injection showed that both SCPC10 and Cristobalite induced a more robust fibroblastic and histiocytic reaction, promoting integration and encapsulation of the particle aggregates, leading to a larger bulking effect. Concentrations of Ca, Na, Si, and P ions in the experimental groups were comparable to control animals. Conclusions. This side-by-side examination of urethral bulking agents using a rat animal model and hard tissue histology techniques compared two newly developed bioactive ceramic particles to three of the currently used bulking agents. The local host tissue response and bulking effects of bioceramic particles were superior while also possessing a comparable safety profile.http://dx.doi.org/10.1155/2016/1282531 |
| spellingShingle | Travis K. Mann-Gow Benjamin J. King Ahmed El-Ghannam Christine Knabe-Ducheyne Masatoshi Kida Ole M. Dall Jan Krhut Peter Zvara Novel Bioceramic Urethral Bulking Agents Elicit Improved Host Tissue Responses in a Rat Model Advances in Urology |
| title | Novel Bioceramic Urethral Bulking Agents Elicit Improved Host Tissue Responses in a Rat Model |
| title_full | Novel Bioceramic Urethral Bulking Agents Elicit Improved Host Tissue Responses in a Rat Model |
| title_fullStr | Novel Bioceramic Urethral Bulking Agents Elicit Improved Host Tissue Responses in a Rat Model |
| title_full_unstemmed | Novel Bioceramic Urethral Bulking Agents Elicit Improved Host Tissue Responses in a Rat Model |
| title_short | Novel Bioceramic Urethral Bulking Agents Elicit Improved Host Tissue Responses in a Rat Model |
| title_sort | novel bioceramic urethral bulking agents elicit improved host tissue responses in a rat model |
| url | http://dx.doi.org/10.1155/2016/1282531 |
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