Bacteriocin Production by <i>Lactiplantibacillus plantarum</i> LD1 in Solid-State Fermentation Using Lignocellulosic Substrates
In this study, solid-state fermentation for growth and bacteriocin production by <i>Lactiplantibacillus plantarum</i> LD1 was carried out using wheat bran, a lignocellulosic substrate. This is the first report showing bacteriocin production using <i>L. plantarum</i> LD1 in so...
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2025-04-01
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| author | Pushpa Rani Bijender Singh Santosh Kumar Tiwari |
| author_facet | Pushpa Rani Bijender Singh Santosh Kumar Tiwari |
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| description | In this study, solid-state fermentation for growth and bacteriocin production by <i>Lactiplantibacillus plantarum</i> LD1 was carried out using wheat bran, a lignocellulosic substrate. This is the first report showing bacteriocin production using <i>L. plantarum</i> LD1 in solid-state fermentation. Wheat bran supported higher production of bacteriocin (391.69 ± 12.58 AU/mL) than other substrates. Appropriate conditions were achieved using statistical designs. Significant factors identified by Plackett–Burman Design and their interactions were studied using response surface methodology. Enhanced production of bacteriocin (582.86 ± 0.87 AU/mL) and optimal growth (log<sub>10</sub> CFU/mL 8.56 ± 0.42) were attained in wheat bran medium supplemented with peptone (1.13%), yeast extract (1.13%), glucose (1.56%), and tri-ammonium citrate (0.50%). Growth in non-optimized medium (MRS) was almost similar (log<sub>10</sub> CFU/mL 8.15 ± 0.20), but the bacteriocin production level was lower (391.69 ± 0.58 AU/mL). Bacteriocin production was sustainable using varied quantities of wheat bran, showing the suitability of the optimized bioprocess for large-scale production. The cost for bacteriocin production in the optimized medium was found to be 444,583.60 AU/USD, which is about 4 times more economical than the cost of the commercial MRS medium, 121,497.18 AU/USD). Thus, an almost 1.5-fold improvement in bacteriocin production was achieved using wheat bran as the substrate. The cost of the production medium was reduced by approximately 25%, making the bioprocess economical. |
| format | Article |
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| spelling | doaj-art-e7dc535c1a754696b4aefd6c7b9cbcd72025-08-20T03:13:49ZengMDPI AGFermentation2311-56372025-04-0111423310.3390/fermentation11040233Bacteriocin Production by <i>Lactiplantibacillus plantarum</i> LD1 in Solid-State Fermentation Using Lignocellulosic SubstratesPushpa Rani0Bijender Singh1Santosh Kumar Tiwari2Department of Genetics, Maharshi Dayanand University, Rohtak 124001, Haryana, IndiaDepartment of Biotechnology, Central University of Haryana, Mahendergarh 123031, Haryana, IndiaDepartment of Genetics, Maharshi Dayanand University, Rohtak 124001, Haryana, IndiaIn this study, solid-state fermentation for growth and bacteriocin production by <i>Lactiplantibacillus plantarum</i> LD1 was carried out using wheat bran, a lignocellulosic substrate. This is the first report showing bacteriocin production using <i>L. plantarum</i> LD1 in solid-state fermentation. Wheat bran supported higher production of bacteriocin (391.69 ± 12.58 AU/mL) than other substrates. Appropriate conditions were achieved using statistical designs. Significant factors identified by Plackett–Burman Design and their interactions were studied using response surface methodology. Enhanced production of bacteriocin (582.86 ± 0.87 AU/mL) and optimal growth (log<sub>10</sub> CFU/mL 8.56 ± 0.42) were attained in wheat bran medium supplemented with peptone (1.13%), yeast extract (1.13%), glucose (1.56%), and tri-ammonium citrate (0.50%). Growth in non-optimized medium (MRS) was almost similar (log<sub>10</sub> CFU/mL 8.15 ± 0.20), but the bacteriocin production level was lower (391.69 ± 0.58 AU/mL). Bacteriocin production was sustainable using varied quantities of wheat bran, showing the suitability of the optimized bioprocess for large-scale production. The cost for bacteriocin production in the optimized medium was found to be 444,583.60 AU/USD, which is about 4 times more economical than the cost of the commercial MRS medium, 121,497.18 AU/USD). Thus, an almost 1.5-fold improvement in bacteriocin production was achieved using wheat bran as the substrate. The cost of the production medium was reduced by approximately 25%, making the bioprocess economical.https://www.mdpi.com/2311-5637/11/4/233<i>Lactiplantibacillus plantarum</i> LD1bacteriocinPlackett–Burmanwheat bran |
| spellingShingle | Pushpa Rani Bijender Singh Santosh Kumar Tiwari Bacteriocin Production by <i>Lactiplantibacillus plantarum</i> LD1 in Solid-State Fermentation Using Lignocellulosic Substrates Fermentation <i>Lactiplantibacillus plantarum</i> LD1 bacteriocin Plackett–Burman wheat bran |
| title | Bacteriocin Production by <i>Lactiplantibacillus plantarum</i> LD1 in Solid-State Fermentation Using Lignocellulosic Substrates |
| title_full | Bacteriocin Production by <i>Lactiplantibacillus plantarum</i> LD1 in Solid-State Fermentation Using Lignocellulosic Substrates |
| title_fullStr | Bacteriocin Production by <i>Lactiplantibacillus plantarum</i> LD1 in Solid-State Fermentation Using Lignocellulosic Substrates |
| title_full_unstemmed | Bacteriocin Production by <i>Lactiplantibacillus plantarum</i> LD1 in Solid-State Fermentation Using Lignocellulosic Substrates |
| title_short | Bacteriocin Production by <i>Lactiplantibacillus plantarum</i> LD1 in Solid-State Fermentation Using Lignocellulosic Substrates |
| title_sort | bacteriocin production by i lactiplantibacillus plantarum i ld1 in solid state fermentation using lignocellulosic substrates |
| topic | <i>Lactiplantibacillus plantarum</i> LD1 bacteriocin Plackett–Burman wheat bran |
| url | https://www.mdpi.com/2311-5637/11/4/233 |
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