Screening of Red Sea- and Mediterranean Sea-derived Actinomycetes for Antimicrobial and Antitumor activities: LC-ESI-HRMS-based Metabolomics Study
Abstract In reply to the critical requirement for new anti-infective and anticancer agents, this study explores aquatic actinomycetes as a favorable source of naturally occurring bioactive compounds. Using metabolomics and dereplication analysis, we investigated the chemical profiles of promising ei...
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| Format: | Article |
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BMC
2025-06-01
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| Series: | Microbial Cell Factories |
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| Online Access: | https://doi.org/10.1186/s12934-025-02759-0 |
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| author | Mahmoud A. Abdel-Razik Ahmed F. Azmy Tarek Dishisha Ahmed O. El-Gendy Adlin Afzan Nurkhalida Kamal Ahmed Tawfike Mohamed Sebak |
| author_facet | Mahmoud A. Abdel-Razik Ahmed F. Azmy Tarek Dishisha Ahmed O. El-Gendy Adlin Afzan Nurkhalida Kamal Ahmed Tawfike Mohamed Sebak |
| author_sort | Mahmoud A. Abdel-Razik |
| collection | DOAJ |
| description | Abstract In reply to the critical requirement for new anti-infective and anticancer agents, this study explores aquatic actinomycetes as a favorable source of naturally occurring bioactive compounds. Using metabolomics and dereplication analysis, we investigated the chemical profiles of promising eight actinobacterial isolates out of seventy-eight actinomycetes isolated from the Red Sea and the Mediterranean Sea. The eight isolates (6, 10, 12, 35, 42, 43, 45, and 48) demonstrated cytotoxic activity against the breast adenocarcinoma (MCF-7) cell line and broad-spectrum antimicrobial activity against Gram-negative and Gram-positive microbes. Isolate 10 exhibited the broadest antibacterial activity, while isolates 42, 43, and 45 showed moderate to weak cytotoxic potential. Genetic identification via 16S rRNA sequencing classified seven isolates as Streptomyces sp. and one as Brevibacterium sp. Multivariate data analysis (MVDA) of the high-resolution mass spectrometry (HRMS) data identified isolate 10 as an outlier, rich in diverse secondary metabolites. Dereplication revealed numerous metabolites with potential antimicrobial and anticancer activity, confirmed by NMR data highlighting their complex chemical profiles. These findings underscore the effectiveness of integrating metabolomics and bioassays to uncover unique bioactive products from aquatic actinobacteria, creating a foundation for further development of novel antimicrobial and antitumor medicines. |
| format | Article |
| id | doaj-art-e7d74f7938a54ab9bef64b6f924bd877 |
| institution | Kabale University |
| issn | 1475-2859 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj-art-e7d74f7938a54ab9bef64b6f924bd8772025-08-20T03:47:17ZengBMCMicrobial Cell Factories1475-28592025-06-0124112110.1186/s12934-025-02759-0Screening of Red Sea- and Mediterranean Sea-derived Actinomycetes for Antimicrobial and Antitumor activities: LC-ESI-HRMS-based Metabolomics StudyMahmoud A. Abdel-Razik0Ahmed F. Azmy1Tarek Dishisha2Ahmed O. El-Gendy3Adlin Afzan4Nurkhalida Kamal5Ahmed Tawfike6Mohamed Sebak7Department of Pharmaceutical Microbiology and Immunology, Faculty of Pharmacy, Beni-Suef UniversityDepartment of Pharmaceutical Microbiology and Immunology, Faculty of Pharmacy, Beni-Suef UniversityDepartment of Pharmaceutical Microbiology and Immunology, Faculty of Pharmacy, Beni-Suef UniversityDepartment of Pharmaceutical Microbiology and Immunology, Faculty of Pharmacy, Beni-Suef UniversityPhytochemistry Unit, Herbal Medicine Research Centre, Institute for Medical Research, National Institutes of Health, Ministry of Health MalaysiaInstitute of Systems Biology, Universiti Kebangsaan Malaysia, UKMDepartment of Pharmacognosy, Faculty of Pharmacy, Helwan UniversityDepartment of Pharmaceutical Microbiology and Immunology, Faculty of Pharmacy, Beni-Suef UniversityAbstract In reply to the critical requirement for new anti-infective and anticancer agents, this study explores aquatic actinomycetes as a favorable source of naturally occurring bioactive compounds. Using metabolomics and dereplication analysis, we investigated the chemical profiles of promising eight actinobacterial isolates out of seventy-eight actinomycetes isolated from the Red Sea and the Mediterranean Sea. The eight isolates (6, 10, 12, 35, 42, 43, 45, and 48) demonstrated cytotoxic activity against the breast adenocarcinoma (MCF-7) cell line and broad-spectrum antimicrobial activity against Gram-negative and Gram-positive microbes. Isolate 10 exhibited the broadest antibacterial activity, while isolates 42, 43, and 45 showed moderate to weak cytotoxic potential. Genetic identification via 16S rRNA sequencing classified seven isolates as Streptomyces sp. and one as Brevibacterium sp. Multivariate data analysis (MVDA) of the high-resolution mass spectrometry (HRMS) data identified isolate 10 as an outlier, rich in diverse secondary metabolites. Dereplication revealed numerous metabolites with potential antimicrobial and anticancer activity, confirmed by NMR data highlighting their complex chemical profiles. These findings underscore the effectiveness of integrating metabolomics and bioassays to uncover unique bioactive products from aquatic actinobacteria, creating a foundation for further development of novel antimicrobial and antitumor medicines.https://doi.org/10.1186/s12934-025-02759-0AntimicrobialCytotoxicityDereplicationLC-HRMSMarine actinomycetesMetabolomics |
| spellingShingle | Mahmoud A. Abdel-Razik Ahmed F. Azmy Tarek Dishisha Ahmed O. El-Gendy Adlin Afzan Nurkhalida Kamal Ahmed Tawfike Mohamed Sebak Screening of Red Sea- and Mediterranean Sea-derived Actinomycetes for Antimicrobial and Antitumor activities: LC-ESI-HRMS-based Metabolomics Study Microbial Cell Factories Antimicrobial Cytotoxicity Dereplication LC-HRMS Marine actinomycetes Metabolomics |
| title | Screening of Red Sea- and Mediterranean Sea-derived Actinomycetes for Antimicrobial and Antitumor activities: LC-ESI-HRMS-based Metabolomics Study |
| title_full | Screening of Red Sea- and Mediterranean Sea-derived Actinomycetes for Antimicrobial and Antitumor activities: LC-ESI-HRMS-based Metabolomics Study |
| title_fullStr | Screening of Red Sea- and Mediterranean Sea-derived Actinomycetes for Antimicrobial and Antitumor activities: LC-ESI-HRMS-based Metabolomics Study |
| title_full_unstemmed | Screening of Red Sea- and Mediterranean Sea-derived Actinomycetes for Antimicrobial and Antitumor activities: LC-ESI-HRMS-based Metabolomics Study |
| title_short | Screening of Red Sea- and Mediterranean Sea-derived Actinomycetes for Antimicrobial and Antitumor activities: LC-ESI-HRMS-based Metabolomics Study |
| title_sort | screening of red sea and mediterranean sea derived actinomycetes for antimicrobial and antitumor activities lc esi hrms based metabolomics study |
| topic | Antimicrobial Cytotoxicity Dereplication LC-HRMS Marine actinomycetes Metabolomics |
| url | https://doi.org/10.1186/s12934-025-02759-0 |
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