Circulating IL6 is involved in the infiltration of M2 macrophages and CD8+ T cells
Abstract To elucidate the relationship between circulating cytokines and the prognosis of microsatellite-stable(MSS) colorectal cancer (CRC) patients, we examined the correlation between circulating cytokine levels and tumor immune infiltration microenvironment in this patient population. By conduct...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-025-92817-9 |
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| author | Jing Huang Rui Xiao Suyujie Shi Qingshu Li Ming Li Ming Xiao Yalan Wang Yaying Yang Wenwen Li Yi Tang |
| author_facet | Jing Huang Rui Xiao Suyujie Shi Qingshu Li Ming Li Ming Xiao Yalan Wang Yaying Yang Wenwen Li Yi Tang |
| author_sort | Jing Huang |
| collection | DOAJ |
| description | Abstract To elucidate the relationship between circulating cytokines and the prognosis of microsatellite-stable(MSS) colorectal cancer (CRC) patients, we examined the correlation between circulating cytokine levels and tumor immune infiltration microenvironment in this patient population. By conducting a preliminary analysis of the GEO database, we identified five core genes associated with colorectal cancer and further analyzed their impact on immune infiltration. We measured serum cytokine levels and validated the immune infiltration results through immunohistochemical staining of common inflammatory cell markers, including CD3, CD4, CD8, CD163, and FOXP3. Our findings indicate that serum cytokine levels significantly influence immune infiltration in colorectal cancer, particularly IL6 and IFNγ, which play crucial roles. Specifically, the infiltration of M2-type macrophages and CD8 + T cells is correlated with serum levels of IL6 and IFNγ. MSS CRC patients with elevated IL6 expression exhibit improved prognosis. |
| format | Article |
| id | doaj-art-e7d3ce9b2ae047bdb5ba1ac5eb0a0297 |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-e7d3ce9b2ae047bdb5ba1ac5eb0a02972025-08-20T02:56:19ZengNature PortfolioScientific Reports2045-23222025-03-0115111510.1038/s41598-025-92817-9Circulating IL6 is involved in the infiltration of M2 macrophages and CD8+ T cellsJing Huang0Rui Xiao1Suyujie Shi2Qingshu Li3Ming Li4Ming Xiao5Yalan Wang6Yaying Yang7Wenwen Li8Yi Tang9Department of Pathology, College of Basic Medicine, Chongqing Medical UniversityDepartment of Pathology, College of Basic Medicine, Chongqing Medical UniversityDepartment of Pathology, College of Basic Medicine, Chongqing Medical UniversityDepartment of Pathology, College of Basic Medicine, Chongqing Medical UniversityDepartment of Pathology, College of Basic Medicine, Chongqing Medical UniversityDepartment of Pathology, College of Basic Medicine, Chongqing Medical UniversityDepartment of Pathology, College of Basic Medicine, Chongqing Medical UniversityDepartment of Pathology, College of Basic Medicine, Chongqing Medical UniversityDepartment of Pathology, College of Basic Medicine, Chongqing Medical UniversityDepartment of Pathology, College of Basic Medicine, Chongqing Medical UniversityAbstract To elucidate the relationship between circulating cytokines and the prognosis of microsatellite-stable(MSS) colorectal cancer (CRC) patients, we examined the correlation between circulating cytokine levels and tumor immune infiltration microenvironment in this patient population. By conducting a preliminary analysis of the GEO database, we identified five core genes associated with colorectal cancer and further analyzed their impact on immune infiltration. We measured serum cytokine levels and validated the immune infiltration results through immunohistochemical staining of common inflammatory cell markers, including CD3, CD4, CD8, CD163, and FOXP3. Our findings indicate that serum cytokine levels significantly influence immune infiltration in colorectal cancer, particularly IL6 and IFNγ, which play crucial roles. Specifically, the infiltration of M2-type macrophages and CD8 + T cells is correlated with serum levels of IL6 and IFNγ. MSS CRC patients with elevated IL6 expression exhibit improved prognosis.https://doi.org/10.1038/s41598-025-92817-9 |
| spellingShingle | Jing Huang Rui Xiao Suyujie Shi Qingshu Li Ming Li Ming Xiao Yalan Wang Yaying Yang Wenwen Li Yi Tang Circulating IL6 is involved in the infiltration of M2 macrophages and CD8+ T cells Scientific Reports |
| title | Circulating IL6 is involved in the infiltration of M2 macrophages and CD8+ T cells |
| title_full | Circulating IL6 is involved in the infiltration of M2 macrophages and CD8+ T cells |
| title_fullStr | Circulating IL6 is involved in the infiltration of M2 macrophages and CD8+ T cells |
| title_full_unstemmed | Circulating IL6 is involved in the infiltration of M2 macrophages and CD8+ T cells |
| title_short | Circulating IL6 is involved in the infiltration of M2 macrophages and CD8+ T cells |
| title_sort | circulating il6 is involved in the infiltration of m2 macrophages and cd8 t cells |
| url | https://doi.org/10.1038/s41598-025-92817-9 |
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