CRISPR/Cas: An Emerging Toolbox for Engineering Virus Resistance in Plants
Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas have been recognized as powerful genome-editing tools in diverse eukaryotic species, including plants, and thus hold great promise for engineering virus resistance in plants. Nevertheless, further attention is required regarding...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-11-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/13/23/3313 |
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| author | Xiaohui Zhan Fengjuan Zhang Ning Li Kai Xu Xiaodi Wang Shenghua Gao Yanxu Yin Weiling Yuan Weifang Chen Zhiyong Ren Minghua Yao Fei Wang |
| author_facet | Xiaohui Zhan Fengjuan Zhang Ning Li Kai Xu Xiaodi Wang Shenghua Gao Yanxu Yin Weiling Yuan Weifang Chen Zhiyong Ren Minghua Yao Fei Wang |
| author_sort | Xiaohui Zhan |
| collection | DOAJ |
| description | Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas have been recognized as powerful genome-editing tools in diverse eukaryotic species, including plants, and thus hold great promise for engineering virus resistance in plants. Nevertheless, further attention is required regarding various issues associated with applying new powerful technologies in the field. This mini-review focuses on the recent advances in using CRISPR/Cas9 and CRISPR/Cas13 systems to combat DNA and RNA viruses in plants. We explored the utility of CRISPR/Cas for targeting the viral genome and editing host susceptibility genes in plants. We also provide insights into the limitations and challenges of using CRISPR/Cas for plant virus interference and propose individual combinatorial solutions. In conclusion, CRISPR/Cas technology has the potential to offer innovative and highly efficient approaches for controlling viruses in important crops in the near future. |
| format | Article |
| id | doaj-art-e7d37e872af54f26a82126eadf4e2fd7 |
| institution | DOAJ |
| issn | 2223-7747 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj-art-e7d37e872af54f26a82126eadf4e2fd72025-08-20T02:50:40ZengMDPI AGPlants2223-77472024-11-011323331310.3390/plants13233313CRISPR/Cas: An Emerging Toolbox for Engineering Virus Resistance in PlantsXiaohui Zhan0Fengjuan Zhang1Ning Li2Kai Xu3Xiaodi Wang4Shenghua Gao5Yanxu Yin6Weiling Yuan7Weifang Chen8Zhiyong Ren9Minghua Yao10Fei Wang11Hubei Key Laboratory of Vegetable Germplasm Innovation and Genetic Improvement, Cash Crops Research Institute, Hubei Academy of Agricultural Sciences, Wuhan 430062, ChinaState Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430062, ChinaHubei Key Laboratory of Vegetable Germplasm Innovation and Genetic Improvement, Cash Crops Research Institute, Hubei Academy of Agricultural Sciences, Wuhan 430062, ChinaHubei Key Laboratory of Vegetable Germplasm Innovation and Genetic Improvement, Cash Crops Research Institute, Hubei Academy of Agricultural Sciences, Wuhan 430062, ChinaHubei Key Laboratory of Vegetable Germplasm Innovation and Genetic Improvement, Cash Crops Research Institute, Hubei Academy of Agricultural Sciences, Wuhan 430062, ChinaHubei Key Laboratory of Vegetable Germplasm Innovation and Genetic Improvement, Cash Crops Research Institute, Hubei Academy of Agricultural Sciences, Wuhan 430062, ChinaHubei Key Laboratory of Vegetable Germplasm Innovation and Genetic Improvement, Cash Crops Research Institute, Hubei Academy of Agricultural Sciences, Wuhan 430062, ChinaHubei Key Laboratory of Vegetable Germplasm Innovation and Genetic Improvement, Cash Crops Research Institute, Hubei Academy of Agricultural Sciences, Wuhan 430062, ChinaHubei Key Laboratory of Vegetable Germplasm Innovation and Genetic Improvement, Cash Crops Research Institute, Hubei Academy of Agricultural Sciences, Wuhan 430062, ChinaHubei Key Laboratory of Vegetable Germplasm Innovation and Genetic Improvement, Cash Crops Research Institute, Hubei Academy of Agricultural Sciences, Wuhan 430062, ChinaHubei Key Laboratory of Vegetable Germplasm Innovation and Genetic Improvement, Cash Crops Research Institute, Hubei Academy of Agricultural Sciences, Wuhan 430062, ChinaHubei Key Laboratory of Vegetable Germplasm Innovation and Genetic Improvement, Cash Crops Research Institute, Hubei Academy of Agricultural Sciences, Wuhan 430062, ChinaClustered regularly interspaced short palindromic repeats (CRISPR)/Cas have been recognized as powerful genome-editing tools in diverse eukaryotic species, including plants, and thus hold great promise for engineering virus resistance in plants. Nevertheless, further attention is required regarding various issues associated with applying new powerful technologies in the field. This mini-review focuses on the recent advances in using CRISPR/Cas9 and CRISPR/Cas13 systems to combat DNA and RNA viruses in plants. We explored the utility of CRISPR/Cas for targeting the viral genome and editing host susceptibility genes in plants. We also provide insights into the limitations and challenges of using CRISPR/Cas for plant virus interference and propose individual combinatorial solutions. In conclusion, CRISPR/Cas technology has the potential to offer innovative and highly efficient approaches for controlling viruses in important crops in the near future.https://www.mdpi.com/2223-7747/13/23/3313CRISPR/Cas9CRISPR/Cas13virus resistance<i>Geminivirus</i><i>Potyvirus</i><i>eIF4E</i> |
| spellingShingle | Xiaohui Zhan Fengjuan Zhang Ning Li Kai Xu Xiaodi Wang Shenghua Gao Yanxu Yin Weiling Yuan Weifang Chen Zhiyong Ren Minghua Yao Fei Wang CRISPR/Cas: An Emerging Toolbox for Engineering Virus Resistance in Plants Plants CRISPR/Cas9 CRISPR/Cas13 virus resistance <i>Geminivirus</i> <i>Potyvirus</i> <i>eIF4E</i> |
| title | CRISPR/Cas: An Emerging Toolbox for Engineering Virus Resistance in Plants |
| title_full | CRISPR/Cas: An Emerging Toolbox for Engineering Virus Resistance in Plants |
| title_fullStr | CRISPR/Cas: An Emerging Toolbox for Engineering Virus Resistance in Plants |
| title_full_unstemmed | CRISPR/Cas: An Emerging Toolbox for Engineering Virus Resistance in Plants |
| title_short | CRISPR/Cas: An Emerging Toolbox for Engineering Virus Resistance in Plants |
| title_sort | crispr cas an emerging toolbox for engineering virus resistance in plants |
| topic | CRISPR/Cas9 CRISPR/Cas13 virus resistance <i>Geminivirus</i> <i>Potyvirus</i> <i>eIF4E</i> |
| url | https://www.mdpi.com/2223-7747/13/23/3313 |
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