CeRNA network reveals potential diagnostic biomarkers or immunotherapy targets for Hypopharyngeal squamous cell carcinoma
Objective: This study aimed to construct a ceRNA network and identify potential diagnostic biomarkers for HSCC. Methods: The R software was used to identify Differentially Expressed circular RNAs (DE-circRNAs) and mRNAs (DE-mRNAs) between HSCC and Normal Control (NC) specimens. Univariate Cox analys...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-09-01
|
| Series: | Brazilian Journal of Otorhinolaryngology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1808869425000989 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849417580541902848 |
|---|---|
| author | Xi Yang Chun Feng Donghui Jiang Xin Xu Yingying Zhang Jin Wang Xiaoguang He |
| author_facet | Xi Yang Chun Feng Donghui Jiang Xin Xu Yingying Zhang Jin Wang Xiaoguang He |
| author_sort | Xi Yang |
| collection | DOAJ |
| description | Objective: This study aimed to construct a ceRNA network and identify potential diagnostic biomarkers for HSCC. Methods: The R software was used to identify Differentially Expressed circular RNAs (DE-circRNAs) and mRNAs (DE-mRNAs) between HSCC and Normal Control (NC) specimens. Univariate Cox analysis and survival curves were used to identify biomarkers associated with the prognosis of HSCC. In addition, the diagnostic value of the biomarkers and their relevance to the tumor microenvironment and immunotherapy were evaluated. A ceRNA network based on mRNAs, circRNAs, and miRNAs was established. Finally, qRT-PCR was used to evaluate the expression of biomarkers in HSCC and para-cancerous tissue samples. Results: A network containing 90 miRNAs, 47 circRNAs, and 111 mRNAs was established via bioinformatic analysis. Three genes (NRG1, CCNG2, and CHSY1) were identified as prognostic and diagnostic biomarkers for HSCC. The low-CCNG2-expression and low-CHSY1-expression groups had higher survival rates, whereas the low-NRG1-expression group had a lower survival rate. The AUC values of the three biomarkers > 0.9, which indicated that the diagnostic value of all biomarkers was excellent. Moreover, CCNG2 and CHSY1 were associated with the severity of malignancy and the effectiveness of immunotherapy. Subsequently, a ceRNA network containing 3 mRNAs, 7 miRNAs, and 7 circRNAs was established. The results of qRT-PCR validated that the expression patterns of NRG1 and CHSY1 in clinical HSCC and para-cancerous samples were consistent with those observed in the GSE2379 dataset. Conclusion: In this study, we identified three diagnostic biomarkers for HSCC (NRG1, CCNG2, and CHSY1) and established their corresponding ceRNA network via bioinformatic analysis, providing novel insights into the screening and treatment of HSCC. |
| format | Article |
| id | doaj-art-e7d1678b57244db3b447ae1c9dc25b04 |
| institution | Kabale University |
| issn | 1808-8694 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Otorhinolaryngology |
| spelling | doaj-art-e7d1678b57244db3b447ae1c9dc25b042025-08-20T03:32:46ZengElsevierBrazilian Journal of Otorhinolaryngology1808-86942025-09-0191510165510.1016/j.bjorl.2025.101655CeRNA network reveals potential diagnostic biomarkers or immunotherapy targets for Hypopharyngeal squamous cell carcinomaXi Yang0Chun Feng1Donghui Jiang2Xin Xu3Yingying Zhang4Jin Wang5Xiaoguang He6The first affiliated Hospital of Kunming Medical University, The Second Department of Otolaryngology, Head and Neck Surgery, Kunming, Yunnan, ChinaThe Affiliated Hospital of Kunming University of Science and Technology, The First People's Hospital of Yunnan Province, Department of Otolaryngology, Kunming, ChinaThe first affiliated Hospital of Kunming Medical University, The Second Department of Otolaryngology, Head and Neck Surgery, Kunming, Yunnan, ChinaThe first affiliated Hospital of Kunming Medical University, The Second Department of Otolaryngology, Head and Neck Surgery, Kunming, Yunnan, ChinaKunming Medical University, School of Basic Medical Science, Department of Pathology and Pathophysiology, Kunming, Yunnan, ChinaThe Affiliated Hospital of Yunnan University, The Second People's Hospital of Yunnan Province, Department of Otolaryngology, Kunming, ChinaThe first affiliated Hospital of Kunming Medical University, The Second Department of Otolaryngology, Head and Neck Surgery, Kunming, Yunnan, China; Corresponding author.Objective: This study aimed to construct a ceRNA network and identify potential diagnostic biomarkers for HSCC. Methods: The R software was used to identify Differentially Expressed circular RNAs (DE-circRNAs) and mRNAs (DE-mRNAs) between HSCC and Normal Control (NC) specimens. Univariate Cox analysis and survival curves were used to identify biomarkers associated with the prognosis of HSCC. In addition, the diagnostic value of the biomarkers and their relevance to the tumor microenvironment and immunotherapy were evaluated. A ceRNA network based on mRNAs, circRNAs, and miRNAs was established. Finally, qRT-PCR was used to evaluate the expression of biomarkers in HSCC and para-cancerous tissue samples. Results: A network containing 90 miRNAs, 47 circRNAs, and 111 mRNAs was established via bioinformatic analysis. Three genes (NRG1, CCNG2, and CHSY1) were identified as prognostic and diagnostic biomarkers for HSCC. The low-CCNG2-expression and low-CHSY1-expression groups had higher survival rates, whereas the low-NRG1-expression group had a lower survival rate. The AUC values of the three biomarkers > 0.9, which indicated that the diagnostic value of all biomarkers was excellent. Moreover, CCNG2 and CHSY1 were associated with the severity of malignancy and the effectiveness of immunotherapy. Subsequently, a ceRNA network containing 3 mRNAs, 7 miRNAs, and 7 circRNAs was established. The results of qRT-PCR validated that the expression patterns of NRG1 and CHSY1 in clinical HSCC and para-cancerous samples were consistent with those observed in the GSE2379 dataset. Conclusion: In this study, we identified three diagnostic biomarkers for HSCC (NRG1, CCNG2, and CHSY1) and established their corresponding ceRNA network via bioinformatic analysis, providing novel insights into the screening and treatment of HSCC.http://www.sciencedirect.com/science/article/pii/S1808869425000989Hypopharyngeal squamous cell carcinomacircRNAceRNA networkBiomarker |
| spellingShingle | Xi Yang Chun Feng Donghui Jiang Xin Xu Yingying Zhang Jin Wang Xiaoguang He CeRNA network reveals potential diagnostic biomarkers or immunotherapy targets for Hypopharyngeal squamous cell carcinoma Brazilian Journal of Otorhinolaryngology Hypopharyngeal squamous cell carcinoma circRNA ceRNA network Biomarker |
| title | CeRNA network reveals potential diagnostic biomarkers or immunotherapy targets for Hypopharyngeal squamous cell carcinoma |
| title_full | CeRNA network reveals potential diagnostic biomarkers or immunotherapy targets for Hypopharyngeal squamous cell carcinoma |
| title_fullStr | CeRNA network reveals potential diagnostic biomarkers or immunotherapy targets for Hypopharyngeal squamous cell carcinoma |
| title_full_unstemmed | CeRNA network reveals potential diagnostic biomarkers or immunotherapy targets for Hypopharyngeal squamous cell carcinoma |
| title_short | CeRNA network reveals potential diagnostic biomarkers or immunotherapy targets for Hypopharyngeal squamous cell carcinoma |
| title_sort | cerna network reveals potential diagnostic biomarkers or immunotherapy targets for hypopharyngeal squamous cell carcinoma |
| topic | Hypopharyngeal squamous cell carcinoma circRNA ceRNA network Biomarker |
| url | http://www.sciencedirect.com/science/article/pii/S1808869425000989 |
| work_keys_str_mv | AT xiyang cernanetworkrevealspotentialdiagnosticbiomarkersorimmunotherapytargetsforhypopharyngealsquamouscellcarcinoma AT chunfeng cernanetworkrevealspotentialdiagnosticbiomarkersorimmunotherapytargetsforhypopharyngealsquamouscellcarcinoma AT donghuijiang cernanetworkrevealspotentialdiagnosticbiomarkersorimmunotherapytargetsforhypopharyngealsquamouscellcarcinoma AT xinxu cernanetworkrevealspotentialdiagnosticbiomarkersorimmunotherapytargetsforhypopharyngealsquamouscellcarcinoma AT yingyingzhang cernanetworkrevealspotentialdiagnosticbiomarkersorimmunotherapytargetsforhypopharyngealsquamouscellcarcinoma AT jinwang cernanetworkrevealspotentialdiagnosticbiomarkersorimmunotherapytargetsforhypopharyngealsquamouscellcarcinoma AT xiaoguanghe cernanetworkrevealspotentialdiagnosticbiomarkersorimmunotherapytargetsforhypopharyngealsquamouscellcarcinoma |