Supernatants from Newly Isolated <i>Lacticaseibacillus paracasei</i> P4 Ameliorate Adipocyte Metabolism in Differentiated 3T3-L1 Cells
<b>Background:</b> <i>Lacticaseibacillus paracasei</i> (<i>L. paracasei</i>) strains and their postbiotics show potential for managing metabolic disorders such as diabetes and obesity. Two newly isolated <i>L. paracasei</i> strains, M2.1 and P4, were y...
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2024-12-01
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| author | Natalia Grigorova Zhenya Ivanova Valeria Petrova Ekaterina Vachkova Georgi Beev |
| author_facet | Natalia Grigorova Zhenya Ivanova Valeria Petrova Ekaterina Vachkova Georgi Beev |
| author_sort | Natalia Grigorova |
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| description | <b>Background:</b> <i>Lacticaseibacillus paracasei</i> (<i>L. paracasei</i>) strains and their postbiotics show potential for managing metabolic disorders such as diabetes and obesity. Two newly isolated <i>L. paracasei</i> strains, M2.1 and P4, were yielded from <i>Formica rufa</i> anthills in Sinite Kamani National Park, Bulgaria. Their metabolic effects on mature 3T3-L1 adipocytes were investigated. <b>Methods:</b> Mature 3T3-L1 adipocytes were treated for 24 h with 10% (<i>v</i>/<i>v</i>) cell-free supernatants (CFSs) of M2.1 or P4. Two experimental (M2.1, P4) and two control groups (mature, untreated adipocytes and mature adipocytes, treated with 10% (<i>v</i>/<i>v</i>) MRS broth) were analyzed for intracellular lipid accumulation, glucose uptake, and the mRNA expression of lipid metabolism and beta-oxidation-related genes. Fold changes in gene expression were assessed using RT-qPCR. <b>Results:</b> Both M2.1 and P4 CFSs enhanced glucose uptake by over 30% compared to the control. P4 demonstrated a more favorable effect by significantly upregulating adipose triglyceride lipase–patatin-like phospholipase domain containing 2, adiponectin, and peroxisomal beta-oxidation enzymes—acyl-coenzyme A oxidase 1, palmitoyl. Intracellular lipid accumulation increased only with M2.1, while P4 supported improved lipid turnover without promoting excessive lipid storage or lipolysis. <b>Conclusions:</b> P4 CFS exhibits the potential to improve adipocyte metabolism by enhancing glucose uptake, promoting beta-oxidation, and increasing adiponectin expression, offering a promising strategy for managing metabolic dysfunctions. |
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| language | English |
| publishDate | 2024-12-01 |
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| series | Biomedicines |
| spelling | doaj-art-e7cd92949a0d4a1196e03218ece8530b2025-08-20T02:00:54ZengMDPI AGBiomedicines2227-90592024-12-011212278510.3390/biomedicines12122785Supernatants from Newly Isolated <i>Lacticaseibacillus paracasei</i> P4 Ameliorate Adipocyte Metabolism in Differentiated 3T3-L1 CellsNatalia Grigorova0Zhenya Ivanova1Valeria Petrova2Ekaterina Vachkova3Georgi Beev4Department of Pharmacology, Animal Physiology, Biochemistry and Chemistry, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, BulgariaDepartment of Pharmacology, Animal Physiology, Biochemistry and Chemistry, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, BulgariaDepartment of Pharmacology, Animal Physiology, Biochemistry and Chemistry, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, BulgariaDepartment of Pharmacology, Animal Physiology, Biochemistry and Chemistry, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, BulgariaDepartment of Biochemistry, Microbiology and Physics, Faculty of Agriculture, Trakia University, 6000 Stara Zagora, Bulgaria<b>Background:</b> <i>Lacticaseibacillus paracasei</i> (<i>L. paracasei</i>) strains and their postbiotics show potential for managing metabolic disorders such as diabetes and obesity. Two newly isolated <i>L. paracasei</i> strains, M2.1 and P4, were yielded from <i>Formica rufa</i> anthills in Sinite Kamani National Park, Bulgaria. Their metabolic effects on mature 3T3-L1 adipocytes were investigated. <b>Methods:</b> Mature 3T3-L1 adipocytes were treated for 24 h with 10% (<i>v</i>/<i>v</i>) cell-free supernatants (CFSs) of M2.1 or P4. Two experimental (M2.1, P4) and two control groups (mature, untreated adipocytes and mature adipocytes, treated with 10% (<i>v</i>/<i>v</i>) MRS broth) were analyzed for intracellular lipid accumulation, glucose uptake, and the mRNA expression of lipid metabolism and beta-oxidation-related genes. Fold changes in gene expression were assessed using RT-qPCR. <b>Results:</b> Both M2.1 and P4 CFSs enhanced glucose uptake by over 30% compared to the control. P4 demonstrated a more favorable effect by significantly upregulating adipose triglyceride lipase–patatin-like phospholipase domain containing 2, adiponectin, and peroxisomal beta-oxidation enzymes—acyl-coenzyme A oxidase 1, palmitoyl. Intracellular lipid accumulation increased only with M2.1, while P4 supported improved lipid turnover without promoting excessive lipid storage or lipolysis. <b>Conclusions:</b> P4 CFS exhibits the potential to improve adipocyte metabolism by enhancing glucose uptake, promoting beta-oxidation, and increasing adiponectin expression, offering a promising strategy for managing metabolic dysfunctions.https://www.mdpi.com/2227-9059/12/12/2785<i>Lacticaseibacillus paracasei</i> P4 and M2.1 strainspostbioticsadipocyte metabolismglucose uptakebeta-oxidationadiponectin |
| spellingShingle | Natalia Grigorova Zhenya Ivanova Valeria Petrova Ekaterina Vachkova Georgi Beev Supernatants from Newly Isolated <i>Lacticaseibacillus paracasei</i> P4 Ameliorate Adipocyte Metabolism in Differentiated 3T3-L1 Cells Biomedicines <i>Lacticaseibacillus paracasei</i> P4 and M2.1 strains postbiotics adipocyte metabolism glucose uptake beta-oxidation adiponectin |
| title | Supernatants from Newly Isolated <i>Lacticaseibacillus paracasei</i> P4 Ameliorate Adipocyte Metabolism in Differentiated 3T3-L1 Cells |
| title_full | Supernatants from Newly Isolated <i>Lacticaseibacillus paracasei</i> P4 Ameliorate Adipocyte Metabolism in Differentiated 3T3-L1 Cells |
| title_fullStr | Supernatants from Newly Isolated <i>Lacticaseibacillus paracasei</i> P4 Ameliorate Adipocyte Metabolism in Differentiated 3T3-L1 Cells |
| title_full_unstemmed | Supernatants from Newly Isolated <i>Lacticaseibacillus paracasei</i> P4 Ameliorate Adipocyte Metabolism in Differentiated 3T3-L1 Cells |
| title_short | Supernatants from Newly Isolated <i>Lacticaseibacillus paracasei</i> P4 Ameliorate Adipocyte Metabolism in Differentiated 3T3-L1 Cells |
| title_sort | supernatants from newly isolated i lacticaseibacillus paracasei i p4 ameliorate adipocyte metabolism in differentiated 3t3 l1 cells |
| topic | <i>Lacticaseibacillus paracasei</i> P4 and M2.1 strains postbiotics adipocyte metabolism glucose uptake beta-oxidation adiponectin |
| url | https://www.mdpi.com/2227-9059/12/12/2785 |
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