Neutrophil-lymphocyte ratio (NLR); an accurate inflammatory marker to predict diabetic foot ulcer amputation: a matched case-control study

Abstract Background Diabetic foot ulcer (DFU) is a well-known complication of diabetes. The main therapeutic options for treating DFU include surgical debridement. However, conditions such as sensory loss and insufficient blood supply can lead to lower extremity amputations. Inflammatory biomarkers,...

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Main Authors: Faeze Afshar, Morteza Daraie, Fatemeh Mohammadi, Kiana Seifouri, Samira Amin Afshari, Soheil Heidari Some’eh, Amirhossein Yadegar, Parnian Naghavi, Alireza Esteghamati, Soghra Rabizadeh, Mahsa Abbaszadeh, Manouchehr Nakhjavani, Sahar Karimpour Reyhan
Format: Article
Language:English
Published: BMC 2025-04-01
Series:BMC Endocrine Disorders
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Online Access:https://doi.org/10.1186/s12902-025-01941-0
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author Faeze Afshar
Morteza Daraie
Fatemeh Mohammadi
Kiana Seifouri
Samira Amin Afshari
Soheil Heidari Some’eh
Amirhossein Yadegar
Parnian Naghavi
Alireza Esteghamati
Soghra Rabizadeh
Mahsa Abbaszadeh
Manouchehr Nakhjavani
Sahar Karimpour Reyhan
author_facet Faeze Afshar
Morteza Daraie
Fatemeh Mohammadi
Kiana Seifouri
Samira Amin Afshari
Soheil Heidari Some’eh
Amirhossein Yadegar
Parnian Naghavi
Alireza Esteghamati
Soghra Rabizadeh
Mahsa Abbaszadeh
Manouchehr Nakhjavani
Sahar Karimpour Reyhan
author_sort Faeze Afshar
collection DOAJ
description Abstract Background Diabetic foot ulcer (DFU) is a well-known complication of diabetes. The main therapeutic options for treating DFU include surgical debridement. However, conditions such as sensory loss and insufficient blood supply can lead to lower extremity amputations. Inflammatory biomarkers, including the neutrophil‒lymphocyte ratio (NLR) and platelet‒lymphocyte ratio (PLR), have shown promise in predicting the development of diabetes complications. Methods This study included 126 individuals with known DFUs who underwent amputation or debridement surgery during hospitalization between January 2017 and December 2022. The participants were divided into two groups, each containing 63 patients, based on the treatment they received. Analyses were conducted via univariate and multivariate regression models. The linearity of the relationship between each inflammatory index and the risk of amputation was further examined via restricted cubic spline (RCS) curves with four knots. Results Categorical regression analysis showed an elevated risk of amputation in patients with an NLR greater than 6.08, with an OR of 13.090 (95% CI: 5.143–33.320, P < 0.001), compared with those with an NLR less than 6.08. Additionally, patients with a PLR greater than 210 demonstrated a similarly elevated risk of amputation with an OR of 2.31 (95% CI: 1.066‒4.669, P = 0.033); however, those with lymphocyte‒white blood cell ratio (LWR) levels of greater than 0.1265 exhibited reduced likelihood of having amputation (OR: 0.092 (95% CI: 0.038‒0.226, P < 0.001)). Conclusions This study supports that NLR, PLR and LWR may have value as a predictive marker for amputation in patients with DFUs.
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spelling doaj-art-e7cbedb35ec24d2ab357ad5e94113d002025-08-20T02:19:57ZengBMCBMC Endocrine Disorders1472-68232025-04-0125111210.1186/s12902-025-01941-0Neutrophil-lymphocyte ratio (NLR); an accurate inflammatory marker to predict diabetic foot ulcer amputation: a matched case-control studyFaeze Afshar0Morteza Daraie1Fatemeh Mohammadi2Kiana Seifouri3Samira Amin Afshari4Soheil Heidari Some’eh5Amirhossein Yadegar6Parnian Naghavi7Alireza Esteghamati8Soghra Rabizadeh9Mahsa Abbaszadeh10Manouchehr Nakhjavani11Sahar Karimpour Reyhan12Endocrinology and Metabolism Research Center (EMRC), Vali-Asr Hospital, Tehran University of Medical SciencesInternal Medicine Department, Imam Khomeini Hospital, Tehran University of Medical SciencesEndocrinology and Metabolism Research Center (EMRC), Vali-Asr Hospital, Tehran University of Medical SciencesEndocrinology and Metabolism Research Center (EMRC), Vali-Asr Hospital, Tehran University of Medical SciencesEndocrinology and Metabolism Research Center (EMRC), Vali-Asr Hospital, Tehran University of Medical SciencesEndocrinology and Metabolism Research Center (EMRC), Vali-Asr Hospital, Tehran University of Medical SciencesEndocrinology and Metabolism Research Center (EMRC), Vali-Asr Hospital, Tehran University of Medical SciencesEndocrinology and Metabolism Research Center (EMRC), Vali-Asr Hospital, Tehran University of Medical SciencesEndocrinology and Metabolism Research Center (EMRC), Vali-Asr Hospital, Tehran University of Medical SciencesEndocrinology and Metabolism Research Center (EMRC), Vali-Asr Hospital, Tehran University of Medical SciencesEndocrinology and Metabolism Research Center (EMRC), Vali-Asr Hospital, Tehran University of Medical SciencesEndocrinology and Metabolism Research Center (EMRC), Vali-Asr Hospital, Tehran University of Medical SciencesEndocrinology and Metabolism Research Center (EMRC), Vali-Asr Hospital, Tehran University of Medical SciencesAbstract Background Diabetic foot ulcer (DFU) is a well-known complication of diabetes. The main therapeutic options for treating DFU include surgical debridement. However, conditions such as sensory loss and insufficient blood supply can lead to lower extremity amputations. Inflammatory biomarkers, including the neutrophil‒lymphocyte ratio (NLR) and platelet‒lymphocyte ratio (PLR), have shown promise in predicting the development of diabetes complications. Methods This study included 126 individuals with known DFUs who underwent amputation or debridement surgery during hospitalization between January 2017 and December 2022. The participants were divided into two groups, each containing 63 patients, based on the treatment they received. Analyses were conducted via univariate and multivariate regression models. The linearity of the relationship between each inflammatory index and the risk of amputation was further examined via restricted cubic spline (RCS) curves with four knots. Results Categorical regression analysis showed an elevated risk of amputation in patients with an NLR greater than 6.08, with an OR of 13.090 (95% CI: 5.143–33.320, P < 0.001), compared with those with an NLR less than 6.08. Additionally, patients with a PLR greater than 210 demonstrated a similarly elevated risk of amputation with an OR of 2.31 (95% CI: 1.066‒4.669, P = 0.033); however, those with lymphocyte‒white blood cell ratio (LWR) levels of greater than 0.1265 exhibited reduced likelihood of having amputation (OR: 0.092 (95% CI: 0.038‒0.226, P < 0.001)). Conclusions This study supports that NLR, PLR and LWR may have value as a predictive marker for amputation in patients with DFUs.https://doi.org/10.1186/s12902-025-01941-0Diabetic foot ulcerNeutrophil‒lymphocyte ratioPlatelet‒lymphocyte ratioLymphocyte‒white blood cell ratio
spellingShingle Faeze Afshar
Morteza Daraie
Fatemeh Mohammadi
Kiana Seifouri
Samira Amin Afshari
Soheil Heidari Some’eh
Amirhossein Yadegar
Parnian Naghavi
Alireza Esteghamati
Soghra Rabizadeh
Mahsa Abbaszadeh
Manouchehr Nakhjavani
Sahar Karimpour Reyhan
Neutrophil-lymphocyte ratio (NLR); an accurate inflammatory marker to predict diabetic foot ulcer amputation: a matched case-control study
BMC Endocrine Disorders
Diabetic foot ulcer
Neutrophil‒lymphocyte ratio
Platelet‒lymphocyte ratio
Lymphocyte‒white blood cell ratio
title Neutrophil-lymphocyte ratio (NLR); an accurate inflammatory marker to predict diabetic foot ulcer amputation: a matched case-control study
title_full Neutrophil-lymphocyte ratio (NLR); an accurate inflammatory marker to predict diabetic foot ulcer amputation: a matched case-control study
title_fullStr Neutrophil-lymphocyte ratio (NLR); an accurate inflammatory marker to predict diabetic foot ulcer amputation: a matched case-control study
title_full_unstemmed Neutrophil-lymphocyte ratio (NLR); an accurate inflammatory marker to predict diabetic foot ulcer amputation: a matched case-control study
title_short Neutrophil-lymphocyte ratio (NLR); an accurate inflammatory marker to predict diabetic foot ulcer amputation: a matched case-control study
title_sort neutrophil lymphocyte ratio nlr an accurate inflammatory marker to predict diabetic foot ulcer amputation a matched case control study
topic Diabetic foot ulcer
Neutrophil‒lymphocyte ratio
Platelet‒lymphocyte ratio
Lymphocyte‒white blood cell ratio
url https://doi.org/10.1186/s12902-025-01941-0
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