Association of circulating levels of anti-CarP antibodies with disease activity, disability and radiological damage in rheumatoid arthritis patients: an open-label, observational study
Abstract Autoantibodies directed against carbamylated proteins (anti-CarP) have been recently identified as predictors for RA development. The aim of this study was to determine the positivity of anti-CarP antibodies in a real-life cohort of RA patients and their association with radiological damage...
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Nature Portfolio
2025-05-01
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| Online Access: | https://doi.org/10.1038/s41598-025-03464-z |
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| author | Milica Markovic Corrado Campochiaro Branislava Glisic Milan Petronijevic Gorica Ristic Ivan Stanojevic Danilo Vojvodic Naomi Viapiana Marco Matucci-Cerinic Lorenzo Dagna |
| author_facet | Milica Markovic Corrado Campochiaro Branislava Glisic Milan Petronijevic Gorica Ristic Ivan Stanojevic Danilo Vojvodic Naomi Viapiana Marco Matucci-Cerinic Lorenzo Dagna |
| author_sort | Milica Markovic |
| collection | DOAJ |
| description | Abstract Autoantibodies directed against carbamylated proteins (anti-CarP) have been recently identified as predictors for RA development. The aim of this study was to determine the positivity of anti-CarP antibodies in a real-life cohort of RA patients and their association with radiological damage, disability and disease activity. In this open-label, observational, cross-sectional study 69 RA patients and 16 healthy controls (HC) were recruited. The study was approved by institutional Ethical Committee. Circulating levels of anti-CarP antibodies were determined by commercial ELISA anti-CarP quantitative sandwich immunoassay. Articular X-rays were evaluated to define the presence of bone erosions. Disease activity (DAS28-CRP) and disability (HAQ-DI) were assessed. Pearson χ2 test, Wilcoxon test or Kruskall–Wallis test was used. Spearman rank-order correlation coefficient was applied for continuous variables. Multivariable logistic regression model was performed. Anti-CarP positivity was found in 35% of RA patients and in no HC. One quarter of seronegative RA patients were anti-CarP positive. A positive correlation between levels of anti-CarP antibodies with DAS28-CRP (p = 0.0003; Spearman r = 0.4829) and HAQ-DI (p = 0.0003; Spearman r = 0.4253) was found. 87% of anti-CarP positive patients had erosions. At multivariable logistic regression analysis, RA disease activity (OR 1.31, 95% CI [1.14, 1.63]) and circulating levels of anti-CarP antibodies (OR 1.66, 95% CI [1.28, 2.37]) were independent predictors of bone erosions. Our study confirms that anti-CarP antibodies are associated with a more aggressive RA course and are independent predictors of bone erosions. |
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| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-e7cadb3d3f1e40f9b65bb36b1ead12952025-08-20T02:00:07ZengNature PortfolioScientific Reports2045-23222025-05-011511810.1038/s41598-025-03464-zAssociation of circulating levels of anti-CarP antibodies with disease activity, disability and radiological damage in rheumatoid arthritis patients: an open-label, observational studyMilica Markovic0Corrado Campochiaro1Branislava Glisic2Milan Petronijevic3Gorica Ristic4Ivan Stanojevic5Danilo Vojvodic6Naomi Viapiana7Marco Matucci-Cerinic8Lorenzo Dagna9Clinic for Internal Medicine, Rheumatology Division, Clinical Center of MontenegroUnit of Immunology, Rheumatology, Allergy and Rare Diseases (UnIRAR), IRCCS Ospedale San Raffaele and Vita-Salute San Raffaele UniversityDepartment of Rheumatology and Clinical Immunology, Medical Faculty of the Military Medical Academy, University of DefenseDepartment of Rheumatology and Clinical Immunology, Medical Faculty of the Military Medical Academy, University of DefenseDepartment of Rheumatology and Clinical Immunology, Medical Faculty of the Military Medical Academy, University of DefenseMedical Faculty of the Military Medical Academy, Institute for Medical Research of the Military Medical Academy, University of DefenseMedical Faculty of the Military Medical Academy, Institute for Medical Research of the Military Medical Academy, University of DefenseUnit of Immunology, Rheumatology, Allergy and Rare Diseases (UnIRAR), IRCCS Ospedale San Raffaele and Vita-Salute San Raffaele UniversityUnit of Immunology, Rheumatology, Allergy and Rare Diseases (UnIRAR), IRCCS Ospedale San Raffaele and Vita-Salute San Raffaele UniversityUnit of Immunology, Rheumatology, Allergy and Rare Diseases (UnIRAR), IRCCS Ospedale San Raffaele and Vita-Salute San Raffaele UniversityAbstract Autoantibodies directed against carbamylated proteins (anti-CarP) have been recently identified as predictors for RA development. The aim of this study was to determine the positivity of anti-CarP antibodies in a real-life cohort of RA patients and their association with radiological damage, disability and disease activity. In this open-label, observational, cross-sectional study 69 RA patients and 16 healthy controls (HC) were recruited. The study was approved by institutional Ethical Committee. Circulating levels of anti-CarP antibodies were determined by commercial ELISA anti-CarP quantitative sandwich immunoassay. Articular X-rays were evaluated to define the presence of bone erosions. Disease activity (DAS28-CRP) and disability (HAQ-DI) were assessed. Pearson χ2 test, Wilcoxon test or Kruskall–Wallis test was used. Spearman rank-order correlation coefficient was applied for continuous variables. Multivariable logistic regression model was performed. Anti-CarP positivity was found in 35% of RA patients and in no HC. One quarter of seronegative RA patients were anti-CarP positive. A positive correlation between levels of anti-CarP antibodies with DAS28-CRP (p = 0.0003; Spearman r = 0.4829) and HAQ-DI (p = 0.0003; Spearman r = 0.4253) was found. 87% of anti-CarP positive patients had erosions. At multivariable logistic regression analysis, RA disease activity (OR 1.31, 95% CI [1.14, 1.63]) and circulating levels of anti-CarP antibodies (OR 1.66, 95% CI [1.28, 2.37]) were independent predictors of bone erosions. Our study confirms that anti-CarP antibodies are associated with a more aggressive RA course and are independent predictors of bone erosions.https://doi.org/10.1038/s41598-025-03464-zAutoantibodiesBiomarkersAnti-CarP antibodiesRheumatoid arthritis |
| spellingShingle | Milica Markovic Corrado Campochiaro Branislava Glisic Milan Petronijevic Gorica Ristic Ivan Stanojevic Danilo Vojvodic Naomi Viapiana Marco Matucci-Cerinic Lorenzo Dagna Association of circulating levels of anti-CarP antibodies with disease activity, disability and radiological damage in rheumatoid arthritis patients: an open-label, observational study Scientific Reports Autoantibodies Biomarkers Anti-CarP antibodies Rheumatoid arthritis |
| title | Association of circulating levels of anti-CarP antibodies with disease activity, disability and radiological damage in rheumatoid arthritis patients: an open-label, observational study |
| title_full | Association of circulating levels of anti-CarP antibodies with disease activity, disability and radiological damage in rheumatoid arthritis patients: an open-label, observational study |
| title_fullStr | Association of circulating levels of anti-CarP antibodies with disease activity, disability and radiological damage in rheumatoid arthritis patients: an open-label, observational study |
| title_full_unstemmed | Association of circulating levels of anti-CarP antibodies with disease activity, disability and radiological damage in rheumatoid arthritis patients: an open-label, observational study |
| title_short | Association of circulating levels of anti-CarP antibodies with disease activity, disability and radiological damage in rheumatoid arthritis patients: an open-label, observational study |
| title_sort | association of circulating levels of anti carp antibodies with disease activity disability and radiological damage in rheumatoid arthritis patients an open label observational study |
| topic | Autoantibodies Biomarkers Anti-CarP antibodies Rheumatoid arthritis |
| url | https://doi.org/10.1038/s41598-025-03464-z |
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