A Sequence-Dependent DNA Condensation Induced by Prion Protein
Different studies indicated that the prion protein induces hybridization of complementary DNA strands. Cell culture studies showed that the scrapie isoform of prion protein remained bound with the chromosome. In present work, we used an oxazole dye, YOYO, as a reporter to quantitative characterizati...
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| Format: | Article |
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Wiley
2018-01-01
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| Series: | Journal of Nucleic Acids |
| Online Access: | http://dx.doi.org/10.1155/2018/9581021 |
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| author | Alakesh Bera Sajal Biring |
| author_facet | Alakesh Bera Sajal Biring |
| author_sort | Alakesh Bera |
| collection | DOAJ |
| description | Different studies indicated that the prion protein induces hybridization of complementary DNA strands. Cell culture studies showed that the scrapie isoform of prion protein remained bound with the chromosome. In present work, we used an oxazole dye, YOYO, as a reporter to quantitative characterization of the DNA condensation by prion protein. We observe that the prion protein induces greater fluorescence quenching of YOYO intercalated in DNA containing only GC bases compared to the DNA containing four bases whereas the effect of dye bound to DNA containing only AT bases is marginal. DNA-condensing biological polyamines are less effective than prion protein in quenching of DNA-bound YOYO fluorescence. The prion protein induces marginal quenching of fluorescence of the dye bound to oligonucleotides, which are resistant to condensation. The ultrastructural studies with electron microscope also validate the biophysical data. The GC bases of the target DNA are probably responsible for increased condensation in the presence of prion protein. To our knowledge, this is the first report of a human cellular protein inducing a sequence-dependent DNA condensation. The increased condensation of GC-rich DNA by prion protein may suggest a biological function of the prion protein and a role in its pathogenesis. |
| format | Article |
| id | doaj-art-e7cad7651cdc40efb7b89688f79aea0a |
| institution | OA Journals |
| issn | 2090-0201 2090-021X |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
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| series | Journal of Nucleic Acids |
| spelling | doaj-art-e7cad7651cdc40efb7b89688f79aea0a2025-08-20T02:22:15ZengWileyJournal of Nucleic Acids2090-02012090-021X2018-01-01201810.1155/2018/95810219581021A Sequence-Dependent DNA Condensation Induced by Prion ProteinAlakesh Bera0Sajal Biring1Infectiologie Animale et Santé Publique, Institut National de la Recherche Agronomique, 37380 Nouzilly, FranceDepartment of Electronic Engineering and Organic Electronics Research Center, Ming-Chi University of Technology, 84 Gungjuan Rd., Taishan Dist., New Taipei City 24301, TaiwanDifferent studies indicated that the prion protein induces hybridization of complementary DNA strands. Cell culture studies showed that the scrapie isoform of prion protein remained bound with the chromosome. In present work, we used an oxazole dye, YOYO, as a reporter to quantitative characterization of the DNA condensation by prion protein. We observe that the prion protein induces greater fluorescence quenching of YOYO intercalated in DNA containing only GC bases compared to the DNA containing four bases whereas the effect of dye bound to DNA containing only AT bases is marginal. DNA-condensing biological polyamines are less effective than prion protein in quenching of DNA-bound YOYO fluorescence. The prion protein induces marginal quenching of fluorescence of the dye bound to oligonucleotides, which are resistant to condensation. The ultrastructural studies with electron microscope also validate the biophysical data. The GC bases of the target DNA are probably responsible for increased condensation in the presence of prion protein. To our knowledge, this is the first report of a human cellular protein inducing a sequence-dependent DNA condensation. The increased condensation of GC-rich DNA by prion protein may suggest a biological function of the prion protein and a role in its pathogenesis.http://dx.doi.org/10.1155/2018/9581021 |
| spellingShingle | Alakesh Bera Sajal Biring A Sequence-Dependent DNA Condensation Induced by Prion Protein Journal of Nucleic Acids |
| title | A Sequence-Dependent DNA Condensation Induced by Prion Protein |
| title_full | A Sequence-Dependent DNA Condensation Induced by Prion Protein |
| title_fullStr | A Sequence-Dependent DNA Condensation Induced by Prion Protein |
| title_full_unstemmed | A Sequence-Dependent DNA Condensation Induced by Prion Protein |
| title_short | A Sequence-Dependent DNA Condensation Induced by Prion Protein |
| title_sort | sequence dependent dna condensation induced by prion protein |
| url | http://dx.doi.org/10.1155/2018/9581021 |
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