In vitro antioxidant and anticancer activity of Leea indica leaf extracts on human prostate cancer cell lines
Background: To determine the phytochemical constituents, antioxidant, and anticancer activities of Leea indica leaf extracts on DU-145 and PC-3 human prostate cancer cell lines. Methods: Leaf sample was subjected to Soxhlet extraction method with increasing polarity of solvents, namely, chloroform,...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-03-01
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| Series: | Integrative Medicine Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213422016301548 |
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| author | Shridhar C. Ghagane Sridevi I. Puranik Vijay M. Kumbar Rajendra B. Nerli Sunil S. Jalalpure Murigendra B. Hiremath Shivayogeeswar Neelagund Ravindranath Aladakatti |
| author_facet | Shridhar C. Ghagane Sridevi I. Puranik Vijay M. Kumbar Rajendra B. Nerli Sunil S. Jalalpure Murigendra B. Hiremath Shivayogeeswar Neelagund Ravindranath Aladakatti |
| author_sort | Shridhar C. Ghagane |
| collection | DOAJ |
| description | Background: To determine the phytochemical constituents, antioxidant, and anticancer activities of Leea indica leaf extracts on DU-145 and PC-3 human prostate cancer cell lines.
Methods: Leaf sample was subjected to Soxhlet extraction method with increasing polarity of solvents, namely, chloroform, ethyl acetate, methanol, ethanol, and aqueous. Phytochemical screening was done using different biochemical tests. Quantitative analysis for phenol was determined by Folin–Ciocalteu reagent method. The antioxidant activity was tested using 2,2-diphenyl-1-picrylhydrazyl, ferric ion reducing power assay, and phosphomolybdenum assay. In vitro anticancer activity on DU-145 and PC-3 human prostate cancer cell lines was evaluated by (3-(4, 5-dimethyl thiazole-2yl)-2, 5-diphenyl tetrazolium bromide) MTT assay.
Results: Phytochemical screening confirmed the presence of phyto-constituents like alkaloids, flavonoids, glycosides, phenols, lignins, saponins, sterols, tannins, anthraquinone, and reducing sugar. Methanol and ethanol extracts exhibited higher phenolic content as compare to aqueous extract. Antioxidant capacities were shown highest in methanol and ethanol extracts based on the test performed. The methanol and ethanol leaf extracts were found to be selectively cytotoxic in vitro to (DU-145 and PC-3) prostate cancer cell lines with IC50 values 529.44 ± 42.07 μg/mL and 677.11 ± 37.01 μg/mL for DU-145 and 547.55 ± 33.52 μg/mL and 631.99 ± 50.24 μg/mL for PC-3 respectively, while it had no cytotoxic effect on normal mice embryo fibroblast cells.
Conclusion: The results indicate that Leea indica was a promising antioxidant and anticancer agent for DU-145 and PC-3 human prostate cancer cell lines. However, further studies are needed to conclude its therapeutic use. |
| format | Article |
| id | doaj-art-e7c83980d10e48d18412a42198a7f6f5 |
| institution | Kabale University |
| issn | 2213-4220 |
| language | English |
| publishDate | 2017-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Integrative Medicine Research |
| spelling | doaj-art-e7c83980d10e48d18412a42198a7f6f52025-08-20T03:24:43ZengElsevierIntegrative Medicine Research2213-42202017-03-0161798710.1016/j.imr.2017.01.004In vitro antioxidant and anticancer activity of Leea indica leaf extracts on human prostate cancer cell linesShridhar C. Ghagane0Sridevi I. Puranik1Vijay M. Kumbar2Rajendra B. Nerli3Sunil S. Jalalpure4Murigendra B. Hiremath5Shivayogeeswar Neelagund6Ravindranath Aladakatti7Department of Biotechnology and Microbiology, Karnatak University, Dharwad, IndiaDepartment of Biotechnology and Microbiology, Karnatak University, Dharwad, IndiaDr. Prabhakar Kore Basic Science Research Center, KLE University, Belagavi, IndiaDepartment of Urology, KLES Kidney Foundation, KLES Dr. Prabhakar Kore Hospital & Medical Research Center, Belagavi, IndiaDr. Prabhakar Kore Basic Science Research Center, KLE University, Belagavi, IndiaDepartment of Biotechnology and Microbiology, Karnatak University, Dharwad, IndiaDepartment of Biochemistry, Kuvempu university, Shimoga, IndiaCentral Animal Facility, Indian Institute of Science, Bangalore, IndiaBackground: To determine the phytochemical constituents, antioxidant, and anticancer activities of Leea indica leaf extracts on DU-145 and PC-3 human prostate cancer cell lines. Methods: Leaf sample was subjected to Soxhlet extraction method with increasing polarity of solvents, namely, chloroform, ethyl acetate, methanol, ethanol, and aqueous. Phytochemical screening was done using different biochemical tests. Quantitative analysis for phenol was determined by Folin–Ciocalteu reagent method. The antioxidant activity was tested using 2,2-diphenyl-1-picrylhydrazyl, ferric ion reducing power assay, and phosphomolybdenum assay. In vitro anticancer activity on DU-145 and PC-3 human prostate cancer cell lines was evaluated by (3-(4, 5-dimethyl thiazole-2yl)-2, 5-diphenyl tetrazolium bromide) MTT assay. Results: Phytochemical screening confirmed the presence of phyto-constituents like alkaloids, flavonoids, glycosides, phenols, lignins, saponins, sterols, tannins, anthraquinone, and reducing sugar. Methanol and ethanol extracts exhibited higher phenolic content as compare to aqueous extract. Antioxidant capacities were shown highest in methanol and ethanol extracts based on the test performed. The methanol and ethanol leaf extracts were found to be selectively cytotoxic in vitro to (DU-145 and PC-3) prostate cancer cell lines with IC50 values 529.44 ± 42.07 μg/mL and 677.11 ± 37.01 μg/mL for DU-145 and 547.55 ± 33.52 μg/mL and 631.99 ± 50.24 μg/mL for PC-3 respectively, while it had no cytotoxic effect on normal mice embryo fibroblast cells. Conclusion: The results indicate that Leea indica was a promising antioxidant and anticancer agent for DU-145 and PC-3 human prostate cancer cell lines. However, further studies are needed to conclude its therapeutic use.http://www.sciencedirect.com/science/article/pii/S2213422016301548anticancerantioxidantLeea indicaphytochemicalprostate cancer |
| spellingShingle | Shridhar C. Ghagane Sridevi I. Puranik Vijay M. Kumbar Rajendra B. Nerli Sunil S. Jalalpure Murigendra B. Hiremath Shivayogeeswar Neelagund Ravindranath Aladakatti In vitro antioxidant and anticancer activity of Leea indica leaf extracts on human prostate cancer cell lines Integrative Medicine Research anticancer antioxidant Leea indica phytochemical prostate cancer |
| title | In vitro antioxidant and anticancer activity of Leea indica leaf extracts on human prostate cancer cell lines |
| title_full | In vitro antioxidant and anticancer activity of Leea indica leaf extracts on human prostate cancer cell lines |
| title_fullStr | In vitro antioxidant and anticancer activity of Leea indica leaf extracts on human prostate cancer cell lines |
| title_full_unstemmed | In vitro antioxidant and anticancer activity of Leea indica leaf extracts on human prostate cancer cell lines |
| title_short | In vitro antioxidant and anticancer activity of Leea indica leaf extracts on human prostate cancer cell lines |
| title_sort | in vitro antioxidant and anticancer activity of leea indica leaf extracts on human prostate cancer cell lines |
| topic | anticancer antioxidant Leea indica phytochemical prostate cancer |
| url | http://www.sciencedirect.com/science/article/pii/S2213422016301548 |
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