Bacterial volatile organic compounds supplemented with light‐emitting diode lighting enhance antioxidant capacity and delay senescence in broccoli

Abstract Bacterial volatile organic compounds (BVOCs) are biological volatiles that can have a positive effect on the postharvest preservation of fruits and vegetables. In the present study, fumigation of broccoli with BVOCs released by Lysinibacillus fusiformis supplemented with light‐emitting diod...

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Main Authors: Taishan Huang, Junyan Shi, Xiaozhen Yue, Shuzhi Yuan, Xiangbin Xu, Jinhua Zuo, Qing Wang
Format: Article
Language:English
Published: Wiley 2025-01-01
Series:Food Frontiers
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Online Access:https://doi.org/10.1002/fft2.500
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author Taishan Huang
Junyan Shi
Xiaozhen Yue
Shuzhi Yuan
Xiangbin Xu
Jinhua Zuo
Qing Wang
author_facet Taishan Huang
Junyan Shi
Xiaozhen Yue
Shuzhi Yuan
Xiangbin Xu
Jinhua Zuo
Qing Wang
author_sort Taishan Huang
collection DOAJ
description Abstract Bacterial volatile organic compounds (BVOCs) are biological volatiles that can have a positive effect on the postharvest preservation of fruits and vegetables. In the present study, fumigation of broccoli with BVOCs released by Lysinibacillus fusiformis supplemented with light‐emitting diode (LED) white light irradiation reduced the accumulation of reactive oxygen species in broccoli during senescence, enhanced the synthesis and accumulation of antioxidant substances, and increased antioxidant enzyme activity in stored broccoli. Compared to the control group (CK), transcriptomic and metabolic analyses of the composite treatment group (LED‐BVOCs) on the 4 days of storage identified 3/1, 7/0, 1/1, 20/6, and 6/1 differentially expressed genes (DEGs)/differentially abundant metabolites in metabolic pathways related to ascorbic acid, glutathione, glucosinolate, polyphenols, and methionine, respectively, and there were 4 DEGs involved in the regulation of senescence during ethylene signal transduction. It is evident that LED‐BVOCs treatment can effectively regulate the metabolic processes of antioxidants in broccoli during storage. Overall, the LED‐BVOCs treatment improved the antioxidant capacity and reduced ethylene production in stored broccoli, thus delaying senescence. Our study provides new insights on the ability of using BVOCs as biopreservatives to improve the antioxidant capacity and delay senescence of broccoli during storage.
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spelling doaj-art-e7c4c4d374f24ba7b07067ba73c61c092025-08-20T01:55:22ZengWileyFood Frontiers2643-84292025-01-016144846410.1002/fft2.500Bacterial volatile organic compounds supplemented with light‐emitting diode lighting enhance antioxidant capacity and delay senescence in broccoliTaishan Huang0Junyan Shi1Xiaozhen Yue2Shuzhi Yuan3Xiangbin Xu4Jinhua Zuo5Qing Wang6Key Laboratory of the Vegetable Postharvest Treatment of Ministry of Agriculture Beijing Key Laboratory of Fruits and Vegetables Storage and Processing Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China) of Ministry of Agriculture Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture National Engineering Research Center for Vegetables Beijing Academy of Agriculture and Forestry Sciences Beijing ChinaKey Laboratory of the Vegetable Postharvest Treatment of Ministry of Agriculture Beijing Key Laboratory of Fruits and Vegetables Storage and Processing Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China) of Ministry of Agriculture Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture National Engineering Research Center for Vegetables Beijing Academy of Agriculture and Forestry Sciences Beijing ChinaKey Laboratory of the Vegetable Postharvest Treatment of Ministry of Agriculture Beijing Key Laboratory of Fruits and Vegetables Storage and Processing Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China) of Ministry of Agriculture Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture National Engineering Research Center for Vegetables Beijing Academy of Agriculture and Forestry Sciences Beijing ChinaKey Laboratory of the Vegetable Postharvest Treatment of Ministry of Agriculture Beijing Key Laboratory of Fruits and Vegetables Storage and Processing Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China) of Ministry of Agriculture Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture National Engineering Research Center for Vegetables Beijing Academy of Agriculture and Forestry Sciences Beijing ChinaKey Laboratory of Food Nutrition and Functional Food of Hainan Province School of Food science and engineering Hainan University Haikou ChinaKey Laboratory of the Vegetable Postharvest Treatment of Ministry of Agriculture Beijing Key Laboratory of Fruits and Vegetables Storage and Processing Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China) of Ministry of Agriculture Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture National Engineering Research Center for Vegetables Beijing Academy of Agriculture and Forestry Sciences Beijing ChinaKey Laboratory of the Vegetable Postharvest Treatment of Ministry of Agriculture Beijing Key Laboratory of Fruits and Vegetables Storage and Processing Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China) of Ministry of Agriculture Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture National Engineering Research Center for Vegetables Beijing Academy of Agriculture and Forestry Sciences Beijing ChinaAbstract Bacterial volatile organic compounds (BVOCs) are biological volatiles that can have a positive effect on the postharvest preservation of fruits and vegetables. In the present study, fumigation of broccoli with BVOCs released by Lysinibacillus fusiformis supplemented with light‐emitting diode (LED) white light irradiation reduced the accumulation of reactive oxygen species in broccoli during senescence, enhanced the synthesis and accumulation of antioxidant substances, and increased antioxidant enzyme activity in stored broccoli. Compared to the control group (CK), transcriptomic and metabolic analyses of the composite treatment group (LED‐BVOCs) on the 4 days of storage identified 3/1, 7/0, 1/1, 20/6, and 6/1 differentially expressed genes (DEGs)/differentially abundant metabolites in metabolic pathways related to ascorbic acid, glutathione, glucosinolate, polyphenols, and methionine, respectively, and there were 4 DEGs involved in the regulation of senescence during ethylene signal transduction. It is evident that LED‐BVOCs treatment can effectively regulate the metabolic processes of antioxidants in broccoli during storage. Overall, the LED‐BVOCs treatment improved the antioxidant capacity and reduced ethylene production in stored broccoli, thus delaying senescence. Our study provides new insights on the ability of using BVOCs as biopreservatives to improve the antioxidant capacity and delay senescence of broccoli during storage.https://doi.org/10.1002/fft2.500antioxidantbacterial volatile organic compounds (BVOCs)biopreservationbroccoliethyleneLED irradiation
spellingShingle Taishan Huang
Junyan Shi
Xiaozhen Yue
Shuzhi Yuan
Xiangbin Xu
Jinhua Zuo
Qing Wang
Bacterial volatile organic compounds supplemented with light‐emitting diode lighting enhance antioxidant capacity and delay senescence in broccoli
Food Frontiers
antioxidant
bacterial volatile organic compounds (BVOCs)
biopreservation
broccoli
ethylene
LED irradiation
title Bacterial volatile organic compounds supplemented with light‐emitting diode lighting enhance antioxidant capacity and delay senescence in broccoli
title_full Bacterial volatile organic compounds supplemented with light‐emitting diode lighting enhance antioxidant capacity and delay senescence in broccoli
title_fullStr Bacterial volatile organic compounds supplemented with light‐emitting diode lighting enhance antioxidant capacity and delay senescence in broccoli
title_full_unstemmed Bacterial volatile organic compounds supplemented with light‐emitting diode lighting enhance antioxidant capacity and delay senescence in broccoli
title_short Bacterial volatile organic compounds supplemented with light‐emitting diode lighting enhance antioxidant capacity and delay senescence in broccoli
title_sort bacterial volatile organic compounds supplemented with light emitting diode lighting enhance antioxidant capacity and delay senescence in broccoli
topic antioxidant
bacterial volatile organic compounds (BVOCs)
biopreservation
broccoli
ethylene
LED irradiation
url https://doi.org/10.1002/fft2.500
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