Hybrid layer-by-layer assembly of AuNPs/NSF composite for electrochemical detection of miRNA-196a
Abstract Detection of microRNA-196a (miRNA-196a) is crucial in cancer research, enabling early diagnosis and providing guidance for individualized treatment. In this work, we employed a naturally occurring negatively charged nano silk fibroin (NSF) with high mechanical properties, biocompatibility,...
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| Format: | Article |
| Language: | English |
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Springer
2024-11-01
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| Series: | Discover Nano |
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| Online Access: | https://doi.org/10.1186/s11671-024-04127-0 |
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| author | Jun Ji Gaofan Shi Jiayi Jiang Shu Wu Haoyuan Su Hongzhi Pan Dongdong Zeng |
| author_facet | Jun Ji Gaofan Shi Jiayi Jiang Shu Wu Haoyuan Su Hongzhi Pan Dongdong Zeng |
| author_sort | Jun Ji |
| collection | DOAJ |
| description | Abstract Detection of microRNA-196a (miRNA-196a) is crucial in cancer research, enabling early diagnosis and providing guidance for individualized treatment. In this work, we employed a naturally occurring negatively charged nano silk fibroin (NSF) with high mechanical properties, biocompatibility, and conductivity to be encapsulated with a positively charged gold nanoparticles (AuNPs) were used as film-forming materials for electrostatic layer-by-layer self-assembly to modify the working electrode of the screen-printed carbon electrode (SPCE). Under the optimized experimental conditions, the uniformly distributed AuNPs on the surface of the multilayer film modified SPCE (AuNPs/NSF)5.5/SPCE combined with the sulfhydryl-modified capture probe cp-DNA through gold-sulfur bonds. Furthermore, miRNA-196a is specifically captured through complementary base pairing to achieve highly sensitive and specific detection. (AuNPs/NSF)5.5/SPCE electrode can detect miRNA-196a in a concentration range of 1.0 × 10−13 to 1.0 × 10−6 M, and the calculated detection limit is 4.63 × 10−14 M when the signal-to-noise ratio is 3. The obtained results showed that the (AuNPs/NSF)5.5/SPCE has excellent selectivity and good stability over time. |
| format | Article |
| id | doaj-art-e7b47fbad8e04824872b45bdb88c30fa |
| institution | DOAJ |
| issn | 2731-9229 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Springer |
| record_format | Article |
| series | Discover Nano |
| spelling | doaj-art-e7b47fbad8e04824872b45bdb88c30fa2025-08-20T02:49:18ZengSpringerDiscover Nano2731-92292024-11-0119111110.1186/s11671-024-04127-0Hybrid layer-by-layer assembly of AuNPs/NSF composite for electrochemical detection of miRNA-196aJun Ji0Gaofan Shi1Jiayi Jiang2Shu Wu3Haoyuan Su4Hongzhi Pan5Dongdong Zeng6Shanghai University of Medicine and Health SciencesShanghai University of Medicine and Health SciencesShanghai University of Medicine and Health SciencesShanghai University of Medicine and Health SciencesShanghai University of Medicine and Health SciencesShanghai University of Medicine and Health SciencesShanghai University of Medicine and Health SciencesAbstract Detection of microRNA-196a (miRNA-196a) is crucial in cancer research, enabling early diagnosis and providing guidance for individualized treatment. In this work, we employed a naturally occurring negatively charged nano silk fibroin (NSF) with high mechanical properties, biocompatibility, and conductivity to be encapsulated with a positively charged gold nanoparticles (AuNPs) were used as film-forming materials for electrostatic layer-by-layer self-assembly to modify the working electrode of the screen-printed carbon electrode (SPCE). Under the optimized experimental conditions, the uniformly distributed AuNPs on the surface of the multilayer film modified SPCE (AuNPs/NSF)5.5/SPCE combined with the sulfhydryl-modified capture probe cp-DNA through gold-sulfur bonds. Furthermore, miRNA-196a is specifically captured through complementary base pairing to achieve highly sensitive and specific detection. (AuNPs/NSF)5.5/SPCE electrode can detect miRNA-196a in a concentration range of 1.0 × 10−13 to 1.0 × 10−6 M, and the calculated detection limit is 4.63 × 10−14 M when the signal-to-noise ratio is 3. The obtained results showed that the (AuNPs/NSF)5.5/SPCE has excellent selectivity and good stability over time.https://doi.org/10.1186/s11671-024-04127-0Electrochemical biosensorLayer-by-layer self-assemblyGold nanoparticlesNano silk fibroinMiRNA-196a |
| spellingShingle | Jun Ji Gaofan Shi Jiayi Jiang Shu Wu Haoyuan Su Hongzhi Pan Dongdong Zeng Hybrid layer-by-layer assembly of AuNPs/NSF composite for electrochemical detection of miRNA-196a Discover Nano Electrochemical biosensor Layer-by-layer self-assembly Gold nanoparticles Nano silk fibroin MiRNA-196a |
| title | Hybrid layer-by-layer assembly of AuNPs/NSF composite for electrochemical detection of miRNA-196a |
| title_full | Hybrid layer-by-layer assembly of AuNPs/NSF composite for electrochemical detection of miRNA-196a |
| title_fullStr | Hybrid layer-by-layer assembly of AuNPs/NSF composite for electrochemical detection of miRNA-196a |
| title_full_unstemmed | Hybrid layer-by-layer assembly of AuNPs/NSF composite for electrochemical detection of miRNA-196a |
| title_short | Hybrid layer-by-layer assembly of AuNPs/NSF composite for electrochemical detection of miRNA-196a |
| title_sort | hybrid layer by layer assembly of aunps nsf composite for electrochemical detection of mirna 196a |
| topic | Electrochemical biosensor Layer-by-layer self-assembly Gold nanoparticles Nano silk fibroin MiRNA-196a |
| url | https://doi.org/10.1186/s11671-024-04127-0 |
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