EFFECT OF IMMUNOGLOBULIN Y PURIFIED FROM IMMUNIZED HEN EGGS ON THE GROWTH OF STAPHYLOCOCCUS AUREUS
A novel purification method of egg yolk immunoglobulin (IgY) based on precipitation using agar-PEG was developed. This method was compared with chloroform extraction and polyethylene glycol (PEG) precipitation methods. The results showed the protein contents were high with chloroform method followed...
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| Format: | Article |
| Language: | English |
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College of Veterinary Medicine, University of Basrah, Iraq
2011-12-01
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| Series: | Basrah Journal of Veterinary Research |
| Subjects: | |
| Online Access: | https://bjvr.uobasrah.edu.iq/article_55030_cbd06b1241a9fa57a7d7f877dc4d5035.pdf |
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| _version_ | 1849321407265112064 |
|---|---|
| author | Ali A. Al-Edany |
| author_facet | Ali A. Al-Edany |
| author_sort | Ali A. Al-Edany |
| collection | DOAJ |
| description | A novel purification method of egg yolk immunoglobulin (IgY) based on
precipitation using agar-PEG was developed. This method was compared with
chloroform extraction and polyethylene glycol (PEG) precipitation methods. The
results showed the protein contents were high with chloroform method followed by
agar-PEG then PEG method. The purity of resultant IgY was homogeneous with agar-
PEG method followed by PEG method then chloroform extraction method. The IgY
purified by agar –PEG method, obtained from hens immunized by formalin-treated S.
aureus, showed a significant reduction in bacterial growth and the growth inhibition
was dependent on specific-IgY concentration |
| format | Article |
| id | doaj-art-e7b39edabb8f409395cf86779c45cf29 |
| institution | Kabale University |
| issn | 1813-8497 2410-8456 |
| language | English |
| publishDate | 2011-12-01 |
| publisher | College of Veterinary Medicine, University of Basrah, Iraq |
| record_format | Article |
| series | Basrah Journal of Veterinary Research |
| spelling | doaj-art-e7b39edabb8f409395cf86779c45cf292025-08-20T03:49:45ZengCollege of Veterinary Medicine, University of Basrah, IraqBasrah Journal of Veterinary Research1813-84972410-84562011-12-01102859610.33762/bvetr.2011.5503055030EFFECT OF IMMUNOGLOBULIN Y PURIFIED FROM IMMUNIZED HEN EGGS ON THE GROWTH OF STAPHYLOCOCCUS AUREUSAli A. Al-EdanyA novel purification method of egg yolk immunoglobulin (IgY) based on precipitation using agar-PEG was developed. This method was compared with chloroform extraction and polyethylene glycol (PEG) precipitation methods. The results showed the protein contents were high with chloroform method followed by agar-PEG then PEG method. The purity of resultant IgY was homogeneous with agar- PEG method followed by PEG method then chloroform extraction method. The IgY purified by agar –PEG method, obtained from hens immunized by formalin-treated S. aureus, showed a significant reduction in bacterial growth and the growth inhibition was dependent on specific-IgY concentrationhttps://bjvr.uobasrah.edu.iq/article_55030_cbd06b1241a9fa57a7d7f877dc4d5035.pdfigychloroformaureus |
| spellingShingle | Ali A. Al-Edany EFFECT OF IMMUNOGLOBULIN Y PURIFIED FROM IMMUNIZED HEN EGGS ON THE GROWTH OF STAPHYLOCOCCUS AUREUS Basrah Journal of Veterinary Research igy chloroform aureus |
| title | EFFECT OF IMMUNOGLOBULIN Y PURIFIED FROM IMMUNIZED HEN EGGS ON THE GROWTH OF STAPHYLOCOCCUS AUREUS |
| title_full | EFFECT OF IMMUNOGLOBULIN Y PURIFIED FROM IMMUNIZED HEN EGGS ON THE GROWTH OF STAPHYLOCOCCUS AUREUS |
| title_fullStr | EFFECT OF IMMUNOGLOBULIN Y PURIFIED FROM IMMUNIZED HEN EGGS ON THE GROWTH OF STAPHYLOCOCCUS AUREUS |
| title_full_unstemmed | EFFECT OF IMMUNOGLOBULIN Y PURIFIED FROM IMMUNIZED HEN EGGS ON THE GROWTH OF STAPHYLOCOCCUS AUREUS |
| title_short | EFFECT OF IMMUNOGLOBULIN Y PURIFIED FROM IMMUNIZED HEN EGGS ON THE GROWTH OF STAPHYLOCOCCUS AUREUS |
| title_sort | effect of immunoglobulin y purified from immunized hen eggs on the growth of staphylococcus aureus |
| topic | igy chloroform aureus |
| url | https://bjvr.uobasrah.edu.iq/article_55030_cbd06b1241a9fa57a7d7f877dc4d5035.pdf |
| work_keys_str_mv | AT aliaaledany effectofimmunoglobulinypurifiedfromimmunizedheneggsonthegrowthofstaphylococcusaureus |