EFFECT OF IMMUNOGLOBULIN Y PURIFIED FROM IMMUNIZED HEN EGGS ON THE GROWTH OF STAPHYLOCOCCUS AUREUS
A novel purification method of egg yolk immunoglobulin (IgY) based on precipitation using agar-PEG was developed. This method was compared with chloroform extraction and polyethylene glycol (PEG) precipitation methods. The results showed the protein contents were high with chloroform method followed...
Saved in:
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
College of Veterinary Medicine, University of Basrah, Iraq
2011-12-01
|
| Series: | Basrah Journal of Veterinary Research |
| Subjects: | |
| Online Access: | https://bjvr.uobasrah.edu.iq/article_55030_cbd06b1241a9fa57a7d7f877dc4d5035.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | A novel purification method of egg yolk immunoglobulin (IgY) based on
precipitation using agar-PEG was developed. This method was compared with
chloroform extraction and polyethylene glycol (PEG) precipitation methods. The
results showed the protein contents were high with chloroform method followed by
agar-PEG then PEG method. The purity of resultant IgY was homogeneous with agar-
PEG method followed by PEG method then chloroform extraction method. The IgY
purified by agar –PEG method, obtained from hens immunized by formalin-treated S.
aureus, showed a significant reduction in bacterial growth and the growth inhibition
was dependent on specific-IgY concentration |
|---|---|
| ISSN: | 1813-8497 2410-8456 |