Mechanisms of the cytotoxicity of carbon nanotubes

Probable mechanisms of cytotoxicity of a total fraction of multi-walled carbon nanotubes using spin probe methods (in human erythrocytes and rat hepatocytes) and electrical breakdown (in mouse oocytes) have been studied. Erythrocyte membrane damage induced by carbon nanotubes is developed in time. A...

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Main Authors: M. T. Kartel, V. P. Chernykh, L. V. Ivanov, E. A. Gordienko, S. N. Kovalenko, Yu. I. Gubin, O. A. Nardid, E. I. Smolyaninova
Format: Article
Language:English
Published: Chuiko Institute of Surface Chemistry of NAS of Ukraine 2011-05-01
Series:Хімія, фізика та технологія поверхні
Online Access:https://cpts.com.ua/index.php/cpts/article/view/88
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author M. T. Kartel
V. P. Chernykh
L. V. Ivanov
E. A. Gordienko
S. N. Kovalenko
Yu. I. Gubin
O. A. Nardid
E. I. Smolyaninova
author_facet M. T. Kartel
V. P. Chernykh
L. V. Ivanov
E. A. Gordienko
S. N. Kovalenko
Yu. I. Gubin
O. A. Nardid
E. I. Smolyaninova
author_sort M. T. Kartel
collection DOAJ
description Probable mechanisms of cytotoxicity of a total fraction of multi-walled carbon nanotubes using spin probe methods (in human erythrocytes and rat hepatocytes) and electrical breakdown (in mouse oocytes) have been studied. Erythrocyte membrane damage induced by carbon nanotubes is developed in time. After two-day exposure at 279 K the erythrocyte samples with carbon nanotubes concentrations of 10, 50, 100, and 200 ?g / ml showed a part of damaged erythrocytes of 4, 10, 16, and 25%, respectively. A 4 h exposure of rat liver homogenate with carbon nanotubes at 273 K resulted in a significant decrease in liver homogenate mitochondrial activity. The study of the processes of oocyte electroporation has shown that probable mechanisms of nanotube cytotoxicity are associated with an increase in membrane conductivity, alteration of surface potential, formation of defects within the membrane, and as a consequence, with an increase in membrane permeability. In addition to their capability to affect membrane structural changes, the nanotubes have a capacity to inhibit delicate electrochemical processes in cells.
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institution Kabale University
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publisher Chuiko Institute of Surface Chemistry of NAS of Ukraine
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series Хімія, фізика та технологія поверхні
spelling doaj-art-e7ad54a13d494315a0d2a3d3fae0bd802025-08-20T03:31:14ZengChuiko Institute of Surface Chemistry of NAS of UkraineХімія, фізика та технологія поверхні2079-17042518-12382011-05-0122Mechanisms of the cytotoxicity of carbon nanotubesM. T. Kartel0V. P. Chernykh1L. V. Ivanov2E. A. Gordienko3S. N. Kovalenko4Yu. I. Gubin5O. A. Nardid6E. I. Smolyaninova7Chuiko Institute of Surface Chemistry of National Academy of Sciences of UkraineNational University of Pharmacy, the Ministry of Heals of UkraineNational University of Pharmacy, the Ministry of Heals of UkraineInstitute for Problems of Cryobiology and Cryomedicine of National Academy of Sciences of UkraineNational University of Pharmacy, the Ministry of Heals of UkraineNational University of Pharmacy, the Ministry of Heals of UkraineInstitute for Problems of Cryobiology and Cryomedicine of National Academy of Sciences of UkraineInstitute for Problems of Cryobiology and Cryomedicine of National Academy of Sciences of UkraineProbable mechanisms of cytotoxicity of a total fraction of multi-walled carbon nanotubes using spin probe methods (in human erythrocytes and rat hepatocytes) and electrical breakdown (in mouse oocytes) have been studied. Erythrocyte membrane damage induced by carbon nanotubes is developed in time. After two-day exposure at 279 K the erythrocyte samples with carbon nanotubes concentrations of 10, 50, 100, and 200 ?g / ml showed a part of damaged erythrocytes of 4, 10, 16, and 25%, respectively. A 4 h exposure of rat liver homogenate with carbon nanotubes at 273 K resulted in a significant decrease in liver homogenate mitochondrial activity. The study of the processes of oocyte electroporation has shown that probable mechanisms of nanotube cytotoxicity are associated with an increase in membrane conductivity, alteration of surface potential, formation of defects within the membrane, and as a consequence, with an increase in membrane permeability. In addition to their capability to affect membrane structural changes, the nanotubes have a capacity to inhibit delicate electrochemical processes in cells.https://cpts.com.ua/index.php/cpts/article/view/88
spellingShingle M. T. Kartel
V. P. Chernykh
L. V. Ivanov
E. A. Gordienko
S. N. Kovalenko
Yu. I. Gubin
O. A. Nardid
E. I. Smolyaninova
Mechanisms of the cytotoxicity of carbon nanotubes
Хімія, фізика та технологія поверхні
title Mechanisms of the cytotoxicity of carbon nanotubes
title_full Mechanisms of the cytotoxicity of carbon nanotubes
title_fullStr Mechanisms of the cytotoxicity of carbon nanotubes
title_full_unstemmed Mechanisms of the cytotoxicity of carbon nanotubes
title_short Mechanisms of the cytotoxicity of carbon nanotubes
title_sort mechanisms of the cytotoxicity of carbon nanotubes
url https://cpts.com.ua/index.php/cpts/article/view/88
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AT snkovalenko mechanismsofthecytotoxicityofcarbonnanotubes
AT yuigubin mechanismsofthecytotoxicityofcarbonnanotubes
AT oanardid mechanismsofthecytotoxicityofcarbonnanotubes
AT eismolyaninova mechanismsofthecytotoxicityofcarbonnanotubes