Effect of burnt tire-ash (water-soluble fraction) on blood and histopathological markers in Clarias gariepinus

Aquatic habitats serve as pollutant reservoirs and experience massive pollution disasters due to anthropogenic pressure. The effect of the Water-Soluble Fraction (WSF) of burnt tire ash (BTA) was investigated on Clarias gariepinus juvenile using blood biomarkers as well as liver and gill histology....

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Main Authors: Adewale Adeolu, Roseline Nwose, Joshua Ekpenyong, Md. Simul Bhuyan, Elizabeth Tabi, Stanley Iheanacho, Johnny Ogunji
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2024-01-01
Series:Watershed Ecology and the Environment
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Online Access:http://www.sciencedirect.com/science/article/pii/S2589471424000123
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author Adewale Adeolu
Roseline Nwose
Joshua Ekpenyong
Md. Simul Bhuyan
Elizabeth Tabi
Stanley Iheanacho
Johnny Ogunji
author_facet Adewale Adeolu
Roseline Nwose
Joshua Ekpenyong
Md. Simul Bhuyan
Elizabeth Tabi
Stanley Iheanacho
Johnny Ogunji
author_sort Adewale Adeolu
collection DOAJ
description Aquatic habitats serve as pollutant reservoirs and experience massive pollution disasters due to anthropogenic pressure. The effect of the Water-Soluble Fraction (WSF) of burnt tire ash (BTA) was investigated on Clarias gariepinus juvenile using blood biomarkers as well as liver and gill histology. The model fish were exposed to 5 % (0.56 g/L), 10 % (1.12 g/L) and 20 % (2.24 g/L) of 96-hr LC50 (11.2 g/L) value of BTA sub-chronic doses during the experiment. Hematological indices, the liver, and gill histology were studied throughout the 28-day exposure study, which was followed by a 14-day post-exposure trial. The studies revealed that BTA-exposed fish had reduced red blood cells, packed cell volume, and hemoglobin levels with time, whereas white blood cells and leukocyte numbers increased. Concentration-dependent histopathologic abnormalities such as hyperplasia, telangiectasis, hemorrhage, desquamation, lamellar edema, and vacuolation were observed in the gill of the affected fish, in contrast to the control. Likewise, the presence of histopathologic abnormalities in the liver of BTA-exposed fish, such as vacuolar degeneration, hyperplasia, dilated portal vein, pyknotic nucleus formations, hypertrophy hepatocytes, and aggregated macrophages, was concentration-dependent. Following apparent histologic deformities that persisted in the tissues after recovery, healing from BTA exposure was only partial. C. gariepinus is an essential bioindicator of burnt tire ash’s ecotoxicological impact. Point and non-point traces of burnt-tire ash to the aquatic systems may harm aquatic animal species, thus necessitating preventative actions.
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spelling doaj-art-e7abc61396ac44b9a45e92ae533cdc582025-08-20T02:43:38ZengKeAi Communications Co., Ltd.Watershed Ecology and the Environment2589-47142024-01-01615516410.1016/j.wsee.2024.08.002Effect of burnt tire-ash (water-soluble fraction) on blood and histopathological markers in Clarias gariepinusAdewale Adeolu0Roseline Nwose1Joshua Ekpenyong2Md. Simul Bhuyan3Elizabeth Tabi4Stanley Iheanacho5Johnny Ogunji6Department of Agriculture, Alex Ekwueme Federal University Ndufu Alike, Ebonyi State, NigeriaDepartment of Agriculture, Alex Ekwueme Federal University Ndufu Alike, Ebonyi State, NigeriaDepartment of Fisheries and Aquaculture, Alex Ekwueme Federal University Ndufu Alike, Ebonyi State, NigeriaBangladesh Oceanographic Research Institute, Cox’s Bazar 4730, Bangladesh; Department of Aquatic Resource Management, Sylhet Agricultural University, Sylhet, 3100, BangladeshDepartment of Fisheries and Aquaculture, Alex Ekwueme Federal University Ndufu Alike, Ebonyi State, NigeriaDepartment of Fisheries and Aquaculture, Alex Ekwueme Federal University Ndufu Alike, Ebonyi State, Nigeria; Institute of Animal Breeding and Husbandry, Dept. Marine Aquaculture, Christian-Albrechts-University Kiel, Olshausenstraße 40, 24098 Kiel, Germany.; Fraunhofer IMTE, Fraunhofer Research Institution for Individualized and Cell-Based Medical Engineering, Aquaculture and Aquatic Resources, Hafentörn 3, 25761 Büsum, Germany; Corresponding author.Department of Fisheries and Aquaculture, Alex Ekwueme Federal University Ndufu Alike, Ebonyi State, NigeriaAquatic habitats serve as pollutant reservoirs and experience massive pollution disasters due to anthropogenic pressure. The effect of the Water-Soluble Fraction (WSF) of burnt tire ash (BTA) was investigated on Clarias gariepinus juvenile using blood biomarkers as well as liver and gill histology. The model fish were exposed to 5 % (0.56 g/L), 10 % (1.12 g/L) and 20 % (2.24 g/L) of 96-hr LC50 (11.2 g/L) value of BTA sub-chronic doses during the experiment. Hematological indices, the liver, and gill histology were studied throughout the 28-day exposure study, which was followed by a 14-day post-exposure trial. The studies revealed that BTA-exposed fish had reduced red blood cells, packed cell volume, and hemoglobin levels with time, whereas white blood cells and leukocyte numbers increased. Concentration-dependent histopathologic abnormalities such as hyperplasia, telangiectasis, hemorrhage, desquamation, lamellar edema, and vacuolation were observed in the gill of the affected fish, in contrast to the control. Likewise, the presence of histopathologic abnormalities in the liver of BTA-exposed fish, such as vacuolar degeneration, hyperplasia, dilated portal vein, pyknotic nucleus formations, hypertrophy hepatocytes, and aggregated macrophages, was concentration-dependent. Following apparent histologic deformities that persisted in the tissues after recovery, healing from BTA exposure was only partial. C. gariepinus is an essential bioindicator of burnt tire ash’s ecotoxicological impact. Point and non-point traces of burnt-tire ash to the aquatic systems may harm aquatic animal species, thus necessitating preventative actions.http://www.sciencedirect.com/science/article/pii/S2589471424000123EcotoxicityAutomobile tiresHematologyHistopathologyAfrican Catfish
spellingShingle Adewale Adeolu
Roseline Nwose
Joshua Ekpenyong
Md. Simul Bhuyan
Elizabeth Tabi
Stanley Iheanacho
Johnny Ogunji
Effect of burnt tire-ash (water-soluble fraction) on blood and histopathological markers in Clarias gariepinus
Watershed Ecology and the Environment
Ecotoxicity
Automobile tires
Hematology
Histopathology
African Catfish
title Effect of burnt tire-ash (water-soluble fraction) on blood and histopathological markers in Clarias gariepinus
title_full Effect of burnt tire-ash (water-soluble fraction) on blood and histopathological markers in Clarias gariepinus
title_fullStr Effect of burnt tire-ash (water-soluble fraction) on blood and histopathological markers in Clarias gariepinus
title_full_unstemmed Effect of burnt tire-ash (water-soluble fraction) on blood and histopathological markers in Clarias gariepinus
title_short Effect of burnt tire-ash (water-soluble fraction) on blood and histopathological markers in Clarias gariepinus
title_sort effect of burnt tire ash water soluble fraction on blood and histopathological markers in clarias gariepinus
topic Ecotoxicity
Automobile tires
Hematology
Histopathology
African Catfish
url http://www.sciencedirect.com/science/article/pii/S2589471424000123
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