Inactivation mechanism and kinetics of dielectric barrier discharge plasma against Escherichia coli O157:H7 in phosphate-buffered saline
This study investigated the inactivation mechanism and kinetics of dielectric barrier discharges (DBD) plasma against Escherichia coli O157:H7. DBD plasma exhibited a remarkable bactericidal effect, and the inactivation kinetics was well described with the Weibull model. Using the ambient air as the...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | CyTA - Journal of Food |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19476337.2024.2303450 |
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| author | Liyuan Niu Dianhe Zhang Bohua Wang Qisen Xiang Yanhong Bai |
| author_facet | Liyuan Niu Dianhe Zhang Bohua Wang Qisen Xiang Yanhong Bai |
| author_sort | Liyuan Niu |
| collection | DOAJ |
| description | This study investigated the inactivation mechanism and kinetics of dielectric barrier discharges (DBD) plasma against Escherichia coli O157:H7. DBD plasma exhibited a remarkable bactericidal effect, and the inactivation kinetics was well described with the Weibull model. Using the ambient air as the working gas, surviving E. coli O157:H7 in phosphate-buffered saline achieved an undetectable level (<10 CFU/mL) after treatment at 18 W for 60 s. DBD plasma altered cell morphology, destructed both the outer and inner membranes, and increased intracellular reactive oxygen species levels. Moreover, DBD plasma significantly decreased the pH of the suspended medium, and increased its oxidation reduction potential and active species levels, which could be mainly responsible for microbial inactivation. The presence of glutathione or N-acetyl-L-cysteine weakened the antibacterial activity of DBD plasma due to their reactive oxygen and nitrogen species (RONS) scavenging capacity, demonstrating that RONS generated by DBD plasma played important roles in decontamination. |
| format | Article |
| id | doaj-art-e7ab4d37ed1d437897b6f94b1e37ae5a |
| institution | OA Journals |
| issn | 1947-6337 1947-6345 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | CyTA - Journal of Food |
| spelling | doaj-art-e7ab4d37ed1d437897b6f94b1e37ae5a2025-08-20T02:37:17ZengTaylor & Francis GroupCyTA - Journal of Food1947-63371947-63452024-12-0122110.1080/19476337.2024.2303450Inactivation mechanism and kinetics of dielectric barrier discharge plasma against Escherichia coli O157:H7 in phosphate-buffered salineLiyuan Niu0Dianhe Zhang1Bohua Wang2Qisen Xiang3Yanhong Bai4College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, ChinaCollege of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, ChinaCollege of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, ChinaCollege of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, ChinaCollege of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, ChinaThis study investigated the inactivation mechanism and kinetics of dielectric barrier discharges (DBD) plasma against Escherichia coli O157:H7. DBD plasma exhibited a remarkable bactericidal effect, and the inactivation kinetics was well described with the Weibull model. Using the ambient air as the working gas, surviving E. coli O157:H7 in phosphate-buffered saline achieved an undetectable level (<10 CFU/mL) after treatment at 18 W for 60 s. DBD plasma altered cell morphology, destructed both the outer and inner membranes, and increased intracellular reactive oxygen species levels. Moreover, DBD plasma significantly decreased the pH of the suspended medium, and increased its oxidation reduction potential and active species levels, which could be mainly responsible for microbial inactivation. The presence of glutathione or N-acetyl-L-cysteine weakened the antibacterial activity of DBD plasma due to their reactive oxygen and nitrogen species (RONS) scavenging capacity, demonstrating that RONS generated by DBD plasma played important roles in decontamination.https://www.tandfonline.com/doi/10.1080/19476337.2024.2303450Escherichia coli O157:H7dielectric barrier discharge plasmainactivationreactive oxygen and nitrogen species (RONS)antioxidants |
| spellingShingle | Liyuan Niu Dianhe Zhang Bohua Wang Qisen Xiang Yanhong Bai Inactivation mechanism and kinetics of dielectric barrier discharge plasma against Escherichia coli O157:H7 in phosphate-buffered saline CyTA - Journal of Food Escherichia coli O157:H7 dielectric barrier discharge plasma inactivation reactive oxygen and nitrogen species (RONS) antioxidants |
| title | Inactivation mechanism and kinetics of dielectric barrier discharge plasma against Escherichia coli O157:H7 in phosphate-buffered saline |
| title_full | Inactivation mechanism and kinetics of dielectric barrier discharge plasma against Escherichia coli O157:H7 in phosphate-buffered saline |
| title_fullStr | Inactivation mechanism and kinetics of dielectric barrier discharge plasma against Escherichia coli O157:H7 in phosphate-buffered saline |
| title_full_unstemmed | Inactivation mechanism and kinetics of dielectric barrier discharge plasma against Escherichia coli O157:H7 in phosphate-buffered saline |
| title_short | Inactivation mechanism and kinetics of dielectric barrier discharge plasma against Escherichia coli O157:H7 in phosphate-buffered saline |
| title_sort | inactivation mechanism and kinetics of dielectric barrier discharge plasma against escherichia coli o157 h7 in phosphate buffered saline |
| topic | Escherichia coli O157:H7 dielectric barrier discharge plasma inactivation reactive oxygen and nitrogen species (RONS) antioxidants |
| url | https://www.tandfonline.com/doi/10.1080/19476337.2024.2303450 |
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