Transcriptomic Profiling of Quinoa Reveals Distinct Defense Responses to Exogenous Methyl Jasmonate and Salicylic Acid
Plant defense responses are mediated by hormones such as jasmonic acid (JA) and salicylic acid (SA). JA and SA are known to trigger a range of different defense responses in model plants but little is described in crops like quinoa. Here, we present the first molecular description of JA and SA signa...
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MDPI AG
2025-06-01
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| author | Oscar M. Rollano-Peñaloza Sara Neyrot Jose A. Bravo Barrera Patricia Mollinedo Allan G. Rasmusson |
| author_facet | Oscar M. Rollano-Peñaloza Sara Neyrot Jose A. Bravo Barrera Patricia Mollinedo Allan G. Rasmusson |
| author_sort | Oscar M. Rollano-Peñaloza |
| collection | DOAJ |
| description | Plant defense responses are mediated by hormones such as jasmonic acid (JA) and salicylic acid (SA). JA and SA are known to trigger a range of different defense responses in model plants but little is described in crops like quinoa. Here, we present the first molecular description of JA and SA signaling at the transcriptomic level in quinoa. The transcriptomes of quinoa cv. Kurmi seedlings treated with 100 µM methyl JA or 1 mM SA for 4 h were analyzed, using on average 4.1 million paired-end reads per sample. Quinoa plants treated with JA showed 1246 differentially expressed (DE) genes and plants treated with SA showed 590 DE genes. The response to JA included the induction of genes for the biosynthesis of JA (8/8 genes) and lignin (10/11 genes), and displayed a strong association with treatments with <i>Trichoderma</i> biocontrol agents. The SA treatment triggered the upregulation of genes for the biosynthesis of monoterpenoids and glucosinolates, both having defense properties. Overall, this suggest that JA and SA promotes the biosynthesis of lignin polymers and chemical defense compounds, respectively. Overall, the DE genes identified can be used as molecular markers in quinoa for tracking plant-hormone pathway involvements in defense responses. |
| format | Article |
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| issn | 2223-7747 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
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| series | Plants |
| spelling | doaj-art-e7a6acfc8fbb4454a91e52ed397ae7892025-08-20T02:23:07ZengMDPI AGPlants2223-77472025-06-011411170810.3390/plants14111708Transcriptomic Profiling of Quinoa Reveals Distinct Defense Responses to Exogenous Methyl Jasmonate and Salicylic AcidOscar M. Rollano-Peñaloza0Sara Neyrot1Jose A. Bravo Barrera2Patricia Mollinedo3Allan G. Rasmusson4Department of Biology, Lund University, Kontaktvägen 13, SE-223 62 Lund, SwedenInstituto de Investigaciones Químicas, Universidad Mayor de San Andrés, Campus Universitario Cota Cota c 27, La Paz P.O. Box 12958, BoliviaInstituto de Investigaciones Químicas, Universidad Mayor de San Andrés, Campus Universitario Cota Cota c 27, La Paz P.O. Box 12958, BoliviaInstituto de Investigaciones Químicas, Universidad Mayor de San Andrés, Campus Universitario Cota Cota c 27, La Paz P.O. Box 12958, BoliviaDepartment of Biology, Lund University, Kontaktvägen 13, SE-223 62 Lund, SwedenPlant defense responses are mediated by hormones such as jasmonic acid (JA) and salicylic acid (SA). JA and SA are known to trigger a range of different defense responses in model plants but little is described in crops like quinoa. Here, we present the first molecular description of JA and SA signaling at the transcriptomic level in quinoa. The transcriptomes of quinoa cv. Kurmi seedlings treated with 100 µM methyl JA or 1 mM SA for 4 h were analyzed, using on average 4.1 million paired-end reads per sample. Quinoa plants treated with JA showed 1246 differentially expressed (DE) genes and plants treated with SA showed 590 DE genes. The response to JA included the induction of genes for the biosynthesis of JA (8/8 genes) and lignin (10/11 genes), and displayed a strong association with treatments with <i>Trichoderma</i> biocontrol agents. The SA treatment triggered the upregulation of genes for the biosynthesis of monoterpenoids and glucosinolates, both having defense properties. Overall, this suggest that JA and SA promotes the biosynthesis of lignin polymers and chemical defense compounds, respectively. Overall, the DE genes identified can be used as molecular markers in quinoa for tracking plant-hormone pathway involvements in defense responses.https://www.mdpi.com/2223-7747/14/11/1708jasmonic acidnext-generation sequencingplant hormonesquinoaRNA-seqsalicylic acid |
| spellingShingle | Oscar M. Rollano-Peñaloza Sara Neyrot Jose A. Bravo Barrera Patricia Mollinedo Allan G. Rasmusson Transcriptomic Profiling of Quinoa Reveals Distinct Defense Responses to Exogenous Methyl Jasmonate and Salicylic Acid Plants jasmonic acid next-generation sequencing plant hormones quinoa RNA-seq salicylic acid |
| title | Transcriptomic Profiling of Quinoa Reveals Distinct Defense Responses to Exogenous Methyl Jasmonate and Salicylic Acid |
| title_full | Transcriptomic Profiling of Quinoa Reveals Distinct Defense Responses to Exogenous Methyl Jasmonate and Salicylic Acid |
| title_fullStr | Transcriptomic Profiling of Quinoa Reveals Distinct Defense Responses to Exogenous Methyl Jasmonate and Salicylic Acid |
| title_full_unstemmed | Transcriptomic Profiling of Quinoa Reveals Distinct Defense Responses to Exogenous Methyl Jasmonate and Salicylic Acid |
| title_short | Transcriptomic Profiling of Quinoa Reveals Distinct Defense Responses to Exogenous Methyl Jasmonate and Salicylic Acid |
| title_sort | transcriptomic profiling of quinoa reveals distinct defense responses to exogenous methyl jasmonate and salicylic acid |
| topic | jasmonic acid next-generation sequencing plant hormones quinoa RNA-seq salicylic acid |
| url | https://www.mdpi.com/2223-7747/14/11/1708 |
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