Increasing the concentration of plasma molecules improves the biological activity of platelet-rich plasma for tissue regeneration
Abstract Platelet-rich plasma (PRP) has emerged as a promising therapy in a variety of medical fields. However, it is crucial to go beyond simple platelet concentration and examine the complex molecular composition both inside and outside platelets. The present work studies the effectiveness of a no...
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Nature Portfolio
2025-02-01
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| Online Access: | https://doi.org/10.1038/s41598-025-88918-0 |
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| author | Mikel Sánchez Jon Mercader Ruiz Daniel Marijuán Pinel Pello Sánchez Nicolás Fiz Jorge Guadilla Juan Azofra Maider Beitia Diego Delgado |
| author_facet | Mikel Sánchez Jon Mercader Ruiz Daniel Marijuán Pinel Pello Sánchez Nicolás Fiz Jorge Guadilla Juan Azofra Maider Beitia Diego Delgado |
| author_sort | Mikel Sánchez |
| collection | DOAJ |
| description | Abstract Platelet-rich plasma (PRP) has emerged as a promising therapy in a variety of medical fields. However, it is crucial to go beyond simple platelet concentration and examine the complex molecular composition both inside and outside platelets. The present work studies the effectiveness of a novel type of PRP named ‘balanced protein-concentrate plasma’ (BPCP). Different growth factor (GF) levels were measured using Enzyme Linked Immunosorbent Assay (ELISA), and in addition to the increase in intra-platelet GFs found in standard PRP (sPRP), BPCP also showed a higher concentration of plasmatic protein. Furthermore, extracellular vesicle (EV) concentration was significantly higher in BPCP. Cell proliferation was higher in cells incubated with lysates derived from BPCP compared to those cultured with sPRP. Regarding cell migration capacity, it was found that the process is platelet-dependent. Finally, the anti-inflammatory effect of BPCP was evaluated by inducing an inflammatory environment in M1-type macrophages. Cytokine levels were measured by ELISA following BPCP administration, showing a significant decrease in pro-inflammatory IL-1β, IL-6 and TNF-α. In summary, although further preclinical and clinical studies are needed in order to determine the therapeutic potential of BPCP, this PRP with unique characteristics demonstrates encouraging in vitro results that could potentially enhance tissue regeneration capacity. |
| format | Article |
| id | doaj-art-e79db95ab8c347fdad8d7faafc1e7a03 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-e79db95ab8c347fdad8d7faafc1e7a032025-02-09T12:37:22ZengNature PortfolioScientific Reports2045-23222025-02-0115111610.1038/s41598-025-88918-0Increasing the concentration of plasma molecules improves the biological activity of platelet-rich plasma for tissue regenerationMikel Sánchez0Jon Mercader Ruiz1Daniel Marijuán Pinel2Pello Sánchez3Nicolás Fiz4Jorge Guadilla5Juan Azofra6Maider Beitia7Diego Delgado8Advanced Biological Therapy Unit, Hospital Vithas VitoriaAdvanced Biological Therapy Unit, Hospital Vithas VitoriaAdvanced Biological Therapy Unit, Hospital Vithas VitoriaAdvanced Biological Therapy Unit, Hospital Vithas VitoriaArthroscopic Surgery Unit, Hospital Vithas VitoriaArthroscopic Surgery Unit, Hospital Vithas VitoriaArthroscopic Surgery Unit, Hospital Vithas VitoriaAdvanced Biological Therapy Unit, Hospital Vithas VitoriaAdvanced Biological Therapy Unit, Hospital Vithas VitoriaAbstract Platelet-rich plasma (PRP) has emerged as a promising therapy in a variety of medical fields. However, it is crucial to go beyond simple platelet concentration and examine the complex molecular composition both inside and outside platelets. The present work studies the effectiveness of a novel type of PRP named ‘balanced protein-concentrate plasma’ (BPCP). Different growth factor (GF) levels were measured using Enzyme Linked Immunosorbent Assay (ELISA), and in addition to the increase in intra-platelet GFs found in standard PRP (sPRP), BPCP also showed a higher concentration of plasmatic protein. Furthermore, extracellular vesicle (EV) concentration was significantly higher in BPCP. Cell proliferation was higher in cells incubated with lysates derived from BPCP compared to those cultured with sPRP. Regarding cell migration capacity, it was found that the process is platelet-dependent. Finally, the anti-inflammatory effect of BPCP was evaluated by inducing an inflammatory environment in M1-type macrophages. Cytokine levels were measured by ELISA following BPCP administration, showing a significant decrease in pro-inflammatory IL-1β, IL-6 and TNF-α. In summary, although further preclinical and clinical studies are needed in order to determine the therapeutic potential of BPCP, this PRP with unique characteristics demonstrates encouraging in vitro results that could potentially enhance tissue regeneration capacity.https://doi.org/10.1038/s41598-025-88918-0Platelet-rich-plasmaGrowth factorsBiomoleculesCell proliferationCell migrationAnti-inflammatory effect |
| spellingShingle | Mikel Sánchez Jon Mercader Ruiz Daniel Marijuán Pinel Pello Sánchez Nicolás Fiz Jorge Guadilla Juan Azofra Maider Beitia Diego Delgado Increasing the concentration of plasma molecules improves the biological activity of platelet-rich plasma for tissue regeneration Scientific Reports Platelet-rich-plasma Growth factors Biomolecules Cell proliferation Cell migration Anti-inflammatory effect |
| title | Increasing the concentration of plasma molecules improves the biological activity of platelet-rich plasma for tissue regeneration |
| title_full | Increasing the concentration of plasma molecules improves the biological activity of platelet-rich plasma for tissue regeneration |
| title_fullStr | Increasing the concentration of plasma molecules improves the biological activity of platelet-rich plasma for tissue regeneration |
| title_full_unstemmed | Increasing the concentration of plasma molecules improves the biological activity of platelet-rich plasma for tissue regeneration |
| title_short | Increasing the concentration of plasma molecules improves the biological activity of platelet-rich plasma for tissue regeneration |
| title_sort | increasing the concentration of plasma molecules improves the biological activity of platelet rich plasma for tissue regeneration |
| topic | Platelet-rich-plasma Growth factors Biomolecules Cell proliferation Cell migration Anti-inflammatory effect |
| url | https://doi.org/10.1038/s41598-025-88918-0 |
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