The Effects of Lycopene and Insulin on Histological Changes and the Expression Level of Bcl-2 Family Genes in the Hippocampus of Streptozotocin-Induced Diabetic Rats
The aim of this study was to evaluate the effects of antioxidants lycopene and insulin on histological changes and expression of Bcl-2 family genes in the hippocampus of streptozotocin-induced type 1 diabetic rats. Forty-eight Wistar rats were divided into six groups of control (C), control treated...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-01-01
|
| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2017/4650939 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850167591917584384 |
|---|---|
| author | Masoume Soleymaninejad Seyed Gholamali Joursaraei Farideh Feizi Iraj Jafari Anarkooli |
| author_facet | Masoume Soleymaninejad Seyed Gholamali Joursaraei Farideh Feizi Iraj Jafari Anarkooli |
| author_sort | Masoume Soleymaninejad |
| collection | DOAJ |
| description | The aim of this study was to evaluate the effects of antioxidants lycopene and insulin on histological changes and expression of Bcl-2 family genes in the hippocampus of streptozotocin-induced type 1 diabetic rats. Forty-eight Wistar rats were divided into six groups of control (C), control treated with lycopene (CL), diabetic (D), diabetic treated with insulin (DI), diabetic treated with lycopene (DL), and diabetic treated with insulin and lycopene (DIL). Diabetes was induced by an injection of streptozotocin (60 mg/kg, IP), lycopene (4 mg/kg/day) was given to the lycopene treated groups as gavages, and insulin (Sc, 1-2 U/kg/day) was injected to the groups treated with insulin. The number of hippocampus neurons undergoing cell death in group D had significant differences with groups C and DIL (p<0.001). Furthermore, insulin and lycopene alone or together reduced the expression of Bax, but increased Bcl-2 and Bcl-xL levels in DI, DL, and DIL rats, especially when compared to group D (p<0.001). The ratios of Bax/Bcl-2 and Bax/Bcl-xL in DI, DL, and DIL rats were also reduced (p<0.001). Our results indicate that treatment with insulin and/or lycopene contribute to the prevention of cell death by reducing the expression of proapoptotic genes and increasing the expression of antiapoptotic genes in the hippocampus. |
| format | Article |
| id | doaj-art-e79babda60c7438aac1681b07b155955 |
| institution | OA Journals |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-e79babda60c7438aac1681b07b1559552025-08-20T02:21:10ZengWileyJournal of Diabetes Research2314-67452314-67532017-01-01201710.1155/2017/46509394650939The Effects of Lycopene and Insulin on Histological Changes and the Expression Level of Bcl-2 Family Genes in the Hippocampus of Streptozotocin-Induced Diabetic RatsMasoume Soleymaninejad0Seyed Gholamali Joursaraei1Farideh Feizi2Iraj Jafari Anarkooli3Department of Anatomy, School of Medicine, Babol University of Medical Sciences, Babol, IranDepartment of Anatomy, School of Medicine, Babol University of Medical Sciences, Babol, IranDepartment of Anatomy, School of Medicine, Babol University of Medical Sciences, Babol, IranDepartment of Anatomy, School of Medicine, Zanjan University of Medical Sciences (ZUMS), Zanjan, IranThe aim of this study was to evaluate the effects of antioxidants lycopene and insulin on histological changes and expression of Bcl-2 family genes in the hippocampus of streptozotocin-induced type 1 diabetic rats. Forty-eight Wistar rats were divided into six groups of control (C), control treated with lycopene (CL), diabetic (D), diabetic treated with insulin (DI), diabetic treated with lycopene (DL), and diabetic treated with insulin and lycopene (DIL). Diabetes was induced by an injection of streptozotocin (60 mg/kg, IP), lycopene (4 mg/kg/day) was given to the lycopene treated groups as gavages, and insulin (Sc, 1-2 U/kg/day) was injected to the groups treated with insulin. The number of hippocampus neurons undergoing cell death in group D had significant differences with groups C and DIL (p<0.001). Furthermore, insulin and lycopene alone or together reduced the expression of Bax, but increased Bcl-2 and Bcl-xL levels in DI, DL, and DIL rats, especially when compared to group D (p<0.001). The ratios of Bax/Bcl-2 and Bax/Bcl-xL in DI, DL, and DIL rats were also reduced (p<0.001). Our results indicate that treatment with insulin and/or lycopene contribute to the prevention of cell death by reducing the expression of proapoptotic genes and increasing the expression of antiapoptotic genes in the hippocampus.http://dx.doi.org/10.1155/2017/4650939 |
| spellingShingle | Masoume Soleymaninejad Seyed Gholamali Joursaraei Farideh Feizi Iraj Jafari Anarkooli The Effects of Lycopene and Insulin on Histological Changes and the Expression Level of Bcl-2 Family Genes in the Hippocampus of Streptozotocin-Induced Diabetic Rats Journal of Diabetes Research |
| title | The Effects of Lycopene and Insulin on Histological Changes and the Expression Level of Bcl-2 Family Genes in the Hippocampus of Streptozotocin-Induced Diabetic Rats |
| title_full | The Effects of Lycopene and Insulin on Histological Changes and the Expression Level of Bcl-2 Family Genes in the Hippocampus of Streptozotocin-Induced Diabetic Rats |
| title_fullStr | The Effects of Lycopene and Insulin on Histological Changes and the Expression Level of Bcl-2 Family Genes in the Hippocampus of Streptozotocin-Induced Diabetic Rats |
| title_full_unstemmed | The Effects of Lycopene and Insulin on Histological Changes and the Expression Level of Bcl-2 Family Genes in the Hippocampus of Streptozotocin-Induced Diabetic Rats |
| title_short | The Effects of Lycopene and Insulin on Histological Changes and the Expression Level of Bcl-2 Family Genes in the Hippocampus of Streptozotocin-Induced Diabetic Rats |
| title_sort | effects of lycopene and insulin on histological changes and the expression level of bcl 2 family genes in the hippocampus of streptozotocin induced diabetic rats |
| url | http://dx.doi.org/10.1155/2017/4650939 |
| work_keys_str_mv | AT masoumesoleymaninejad theeffectsoflycopeneandinsulinonhistologicalchangesandtheexpressionlevelofbcl2familygenesinthehippocampusofstreptozotocininduceddiabeticrats AT seyedgholamalijoursaraei theeffectsoflycopeneandinsulinonhistologicalchangesandtheexpressionlevelofbcl2familygenesinthehippocampusofstreptozotocininduceddiabeticrats AT faridehfeizi theeffectsoflycopeneandinsulinonhistologicalchangesandtheexpressionlevelofbcl2familygenesinthehippocampusofstreptozotocininduceddiabeticrats AT irajjafarianarkooli theeffectsoflycopeneandinsulinonhistologicalchangesandtheexpressionlevelofbcl2familygenesinthehippocampusofstreptozotocininduceddiabeticrats AT masoumesoleymaninejad effectsoflycopeneandinsulinonhistologicalchangesandtheexpressionlevelofbcl2familygenesinthehippocampusofstreptozotocininduceddiabeticrats AT seyedgholamalijoursaraei effectsoflycopeneandinsulinonhistologicalchangesandtheexpressionlevelofbcl2familygenesinthehippocampusofstreptozotocininduceddiabeticrats AT faridehfeizi effectsoflycopeneandinsulinonhistologicalchangesandtheexpressionlevelofbcl2familygenesinthehippocampusofstreptozotocininduceddiabeticrats AT irajjafarianarkooli effectsoflycopeneandinsulinonhistologicalchangesandtheexpressionlevelofbcl2familygenesinthehippocampusofstreptozotocininduceddiabeticrats |