A novel phage phaST4: Characterization and its protective potential against Aeromonas hydrophila infection in swamp eels
The rise in antibiotic resistance of Aeromonas hydrophila has attracted increased concern and attention. In the present study, we isolated and characterized a novel phage designated phaST4 from aquaculture sewage. A morphological analysis revealed that phaST4 exhibited a symmetrical icosahedral head...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-09-01
|
| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513425003400 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849392439595368448 |
|---|---|
| author | Jianfen Wu Lifei Chen Sixuan Wu Yi Li Yuhe Zhao Chongyang Lu Wenxiu Sun Wei Li |
| author_facet | Jianfen Wu Lifei Chen Sixuan Wu Yi Li Yuhe Zhao Chongyang Lu Wenxiu Sun Wei Li |
| author_sort | Jianfen Wu |
| collection | DOAJ |
| description | The rise in antibiotic resistance of Aeromonas hydrophila has attracted increased concern and attention. In the present study, we isolated and characterized a novel phage designated phaST4 from aquaculture sewage. A morphological analysis revealed that phaST4 exhibited a symmetrical icosahedral head (85.5 ± 2.8 nm) and a long contractile tail (166 ± 3.3 nm × 22 ± 1.3 nm). Whole genome sequence analysis revealed that phaST4 is a double-stranded DNA phage of 90,646 bp in length. Its genome contains 113 putative open reading frames (ORFs) and 4 tRNAs. Moreover, no known virulence genes or drug-resistant genes were identified. Combined with a gene-sharing networks analysis, the results of sequence comparison and phylogenetic analysis indicated that phaST4 is the first representative of a new species within a previously undefined genus. The optimal multiplicity of infection (MOI) for phaST4 was 0.001, and the latent period and burst size were 20 min and 146 plaque-forming units (PFU)/infected cell, respectively. PhaST4 exhibited stable activity at 30°C–50°C and a pH range of 4–11. In addition, it showed a strong tolerance to chloroform. PhaST4 exhibited efficient biofilm inhibition and clearance capacity to its indicator host, as well as time- and MOI-dependent inhibition toward Aeromonas hydrophila in vitro. In addition, intraperitoneal injection of phaST4 significantly reduced the expression of inflammation-related genes, including NF-kB, IL-1β, and IL-6, in the liver and intestine of swamp eels challenged with A. hydrophila. A histopathological analysis revealed that phaST4 administration ameliorates liver and intestinal injury compared with the bacterial infection groups. Overall, the results indicate that phaST4 has potential use for the treatment of A. hydrophila infection. |
| format | Article |
| id | doaj-art-e79a5011277e46d48ac97815c125c963 |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-e79a5011277e46d48ac97815c125c9632025-08-20T03:40:46ZengElsevierAquaculture Reports2352-51342025-09-014310295410.1016/j.aqrep.2025.102954A novel phage phaST4: Characterization and its protective potential against Aeromonas hydrophila infection in swamp eelsJianfen Wu0Lifei Chen1Sixuan Wu2Yi Li3Yuhe Zhao4Chongyang Lu5Wenxiu Sun6Wei Li7College of Life Science, Yangtze University, Jingzhou 434025, ChinaCollege of Life Science, Yangtze University, Jingzhou 434025, ChinaCollege of Life Science, Yangtze University, Jingzhou 434025, ChinaCollege of Life Science, Yangtze University, Jingzhou 434025, ChinaCollege of Life Science, Yangtze University, Jingzhou 434025, ChinaCollege of Life Science, Yangtze University, Jingzhou 434025, ChinaCollege of Life Science, Yangtze University, Jingzhou 434025, ChinaCorrespondence to: College of Life Science, Yangtze University, Jingmi Road 266, Jingzhou, Hubei province 434025, China.; College of Life Science, Yangtze University, Jingzhou 434025, ChinaThe rise in antibiotic resistance of Aeromonas hydrophila has attracted increased concern and attention. In the present study, we isolated and characterized a novel phage designated phaST4 from aquaculture sewage. A morphological analysis revealed that phaST4 exhibited a symmetrical icosahedral head (85.5 ± 2.8 nm) and a long contractile tail (166 ± 3.3 nm × 22 ± 1.3 nm). Whole genome sequence analysis revealed that phaST4 is a double-stranded DNA phage of 90,646 bp in length. Its genome contains 113 putative open reading frames (ORFs) and 4 tRNAs. Moreover, no known virulence genes or drug-resistant genes were identified. Combined with a gene-sharing networks analysis, the results of sequence comparison and phylogenetic analysis indicated that phaST4 is the first representative of a new species within a previously undefined genus. The optimal multiplicity of infection (MOI) for phaST4 was 0.001, and the latent period and burst size were 20 min and 146 plaque-forming units (PFU)/infected cell, respectively. PhaST4 exhibited stable activity at 30°C–50°C and a pH range of 4–11. In addition, it showed a strong tolerance to chloroform. PhaST4 exhibited efficient biofilm inhibition and clearance capacity to its indicator host, as well as time- and MOI-dependent inhibition toward Aeromonas hydrophila in vitro. In addition, intraperitoneal injection of phaST4 significantly reduced the expression of inflammation-related genes, including NF-kB, IL-1β, and IL-6, in the liver and intestine of swamp eels challenged with A. hydrophila. A histopathological analysis revealed that phaST4 administration ameliorates liver and intestinal injury compared with the bacterial infection groups. Overall, the results indicate that phaST4 has potential use for the treatment of A. hydrophila infection.http://www.sciencedirect.com/science/article/pii/S2352513425003400Aeromonas hydrophilaPhaST4CharacterizationGenomic analysis |
| spellingShingle | Jianfen Wu Lifei Chen Sixuan Wu Yi Li Yuhe Zhao Chongyang Lu Wenxiu Sun Wei Li A novel phage phaST4: Characterization and its protective potential against Aeromonas hydrophila infection in swamp eels Aquaculture Reports Aeromonas hydrophila PhaST4 Characterization Genomic analysis |
| title | A novel phage phaST4: Characterization and its protective potential against Aeromonas hydrophila infection in swamp eels |
| title_full | A novel phage phaST4: Characterization and its protective potential against Aeromonas hydrophila infection in swamp eels |
| title_fullStr | A novel phage phaST4: Characterization and its protective potential against Aeromonas hydrophila infection in swamp eels |
| title_full_unstemmed | A novel phage phaST4: Characterization and its protective potential against Aeromonas hydrophila infection in swamp eels |
| title_short | A novel phage phaST4: Characterization and its protective potential against Aeromonas hydrophila infection in swamp eels |
| title_sort | novel phage phast4 characterization and its protective potential against aeromonas hydrophila infection in swamp eels |
| topic | Aeromonas hydrophila PhaST4 Characterization Genomic analysis |
| url | http://www.sciencedirect.com/science/article/pii/S2352513425003400 |
| work_keys_str_mv | AT jianfenwu anovelphagephast4characterizationanditsprotectivepotentialagainstaeromonashydrophilainfectioninswampeels AT lifeichen anovelphagephast4characterizationanditsprotectivepotentialagainstaeromonashydrophilainfectioninswampeels AT sixuanwu anovelphagephast4characterizationanditsprotectivepotentialagainstaeromonashydrophilainfectioninswampeels AT yili anovelphagephast4characterizationanditsprotectivepotentialagainstaeromonashydrophilainfectioninswampeels AT yuhezhao anovelphagephast4characterizationanditsprotectivepotentialagainstaeromonashydrophilainfectioninswampeels AT chongyanglu anovelphagephast4characterizationanditsprotectivepotentialagainstaeromonashydrophilainfectioninswampeels AT wenxiusun anovelphagephast4characterizationanditsprotectivepotentialagainstaeromonashydrophilainfectioninswampeels AT weili anovelphagephast4characterizationanditsprotectivepotentialagainstaeromonashydrophilainfectioninswampeels AT jianfenwu novelphagephast4characterizationanditsprotectivepotentialagainstaeromonashydrophilainfectioninswampeels AT lifeichen novelphagephast4characterizationanditsprotectivepotentialagainstaeromonashydrophilainfectioninswampeels AT sixuanwu novelphagephast4characterizationanditsprotectivepotentialagainstaeromonashydrophilainfectioninswampeels AT yili novelphagephast4characterizationanditsprotectivepotentialagainstaeromonashydrophilainfectioninswampeels AT yuhezhao novelphagephast4characterizationanditsprotectivepotentialagainstaeromonashydrophilainfectioninswampeels AT chongyanglu novelphagephast4characterizationanditsprotectivepotentialagainstaeromonashydrophilainfectioninswampeels AT wenxiusun novelphagephast4characterizationanditsprotectivepotentialagainstaeromonashydrophilainfectioninswampeels AT weili novelphagephast4characterizationanditsprotectivepotentialagainstaeromonashydrophilainfectioninswampeels |