Comparison of molecular with conventional methods for detection of genital tuberculosis in infertile women: A comparative prospective study
Background: Diagnosis of genital tuberculosis (TB) as a risk factor for infertility remains a diagnostic dilemma for clinicians. Employing the best clinical practices for diagnosing and treating genital TB may help optimize fertility rate. The current study aimed to evaluate the molecular and conven...
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| Format: | Article |
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Golestan University Of Medical Sciences
2025-02-01
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| Series: | Journal of Clinical and Basic Research |
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| Online Access: | http://jcbr.goums.ac.ir/article-1-478-en.pdf |
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| author | Anupama Hari Anusha Reddy Thota Sravanthi Katamreddy |
| author_facet | Anupama Hari Anusha Reddy Thota Sravanthi Katamreddy |
| author_sort | Anupama Hari |
| collection | DOAJ |
| description | Background: Diagnosis of genital tuberculosis (TB) as a risk factor for infertility remains a diagnostic dilemma for clinicians. Employing the best clinical practices for diagnosing and treating genital TB may help optimize fertility rate. The current study aimed to evaluate the molecular and conventional methods to diagnose genital TB in women with infertility.
Methods: A comparative study was conducted on 100 infertile women between 20-35 years of age. Routine laboratory assays and other investigations, such as cervical swab gonorrhea and Chlamydia, Nested TB Polymerase Chain Reaction (TB-PCR), histopathological examination (HPE), culture by Bactec method, and hysterosalpingography (HSG) were performed.
Results: The majority of the cases (52%) were between 21 and 25 years. Moreover, 68% of the participants had patent tubes on HSG, 7% had a right tubal block, 9% had a left tubal block, 14% had a bilateral tubal block, and 2% had a unicornuate uterus. Additionally, 9% of the participants were TB-PCR-positive. Hysterolaparoscopy results suggested a likelihood of TB observed among 8% of the participants. Also, 25% of the patients with TB and 7.9% of the patients without genital TB had positive Mantoux test results. Furthermore, 75% of the patients with genital TB had HSG results suggestive of TB, whereas 23.8% of the patients without genital TB had such results. Laparoscopy (75%) and TB-PCR (75%), as well as HSG (75%), were the most specific tests for ruling out genital TB. The most sensitive tests for diagnosing genital TB were TB-PCR (100%), HPE (100%), and laparoscopy (100%).
Conclusion: Hysterolaparoscopy is useful for early diagnosis of genital TB. PCR is highly helpful in paucibacillary vaginal TB. Positive findings can be obtained with as few as 10 bacteria/ml of material. In addition to clinical and endoscopic examination, the routine use of endometrial bacteriological PCR testing may be significantly promising for enhancing the diagnosis of genital TB. For the best results, early detection and timely treatment are essential. |
| format | Article |
| id | doaj-art-e797cfc2e8964e77bbf4d30bc214a8d7 |
| institution | DOAJ |
| issn | 2538-3736 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Golestan University Of Medical Sciences |
| record_format | Article |
| series | Journal of Clinical and Basic Research |
| spelling | doaj-art-e797cfc2e8964e77bbf4d30bc214a8d72025-08-20T03:15:04ZengGolestan University Of Medical SciencesJournal of Clinical and Basic Research2538-37362025-02-01912428Comparison of molecular with conventional methods for detection of genital tuberculosis in infertile women: A comparative prospective studyAnupama Hari0Anusha Reddy Thota1Sravanthi Katamreddy2 Department of Obstetrics and Gynecology, Gandhi Medical College and Hospital, Secunderabad, India Department of Obstetrics and Gynecology, Mahatma Gandhi Medical College and Research Institute, Pillayarkuppam, Puducherry, India Department of Obstetrics and Gynecology, Santhiram Medical College and General Hospital, Nandyal, India Background: Diagnosis of genital tuberculosis (TB) as a risk factor for infertility remains a diagnostic dilemma for clinicians. Employing the best clinical practices for diagnosing and treating genital TB may help optimize fertility rate. The current study aimed to evaluate the molecular and conventional methods to diagnose genital TB in women with infertility. Methods: A comparative study was conducted on 100 infertile women between 20-35 years of age. Routine laboratory assays and other investigations, such as cervical swab gonorrhea and Chlamydia, Nested TB Polymerase Chain Reaction (TB-PCR), histopathological examination (HPE), culture by Bactec method, and hysterosalpingography (HSG) were performed. Results: The majority of the cases (52%) were between 21 and 25 years. Moreover, 68% of the participants had patent tubes on HSG, 7% had a right tubal block, 9% had a left tubal block, 14% had a bilateral tubal block, and 2% had a unicornuate uterus. Additionally, 9% of the participants were TB-PCR-positive. Hysterolaparoscopy results suggested a likelihood of TB observed among 8% of the participants. Also, 25% of the patients with TB and 7.9% of the patients without genital TB had positive Mantoux test results. Furthermore, 75% of the patients with genital TB had HSG results suggestive of TB, whereas 23.8% of the patients without genital TB had such results. Laparoscopy (75%) and TB-PCR (75%), as well as HSG (75%), were the most specific tests for ruling out genital TB. The most sensitive tests for diagnosing genital TB were TB-PCR (100%), HPE (100%), and laparoscopy (100%). Conclusion: Hysterolaparoscopy is useful for early diagnosis of genital TB. PCR is highly helpful in paucibacillary vaginal TB. Positive findings can be obtained with as few as 10 bacteria/ml of material. In addition to clinical and endoscopic examination, the routine use of endometrial bacteriological PCR testing may be significantly promising for enhancing the diagnosis of genital TB. For the best results, early detection and timely treatment are essential.http://jcbr.goums.ac.ir/article-1-478-en.pdftuberculosisendometritispolymerase chain reactionhysterolaparoscopymantoux |
| spellingShingle | Anupama Hari Anusha Reddy Thota Sravanthi Katamreddy Comparison of molecular with conventional methods for detection of genital tuberculosis in infertile women: A comparative prospective study Journal of Clinical and Basic Research tuberculosis endometritis polymerase chain reaction hysterolaparoscopy mantoux |
| title | Comparison of molecular with conventional methods for detection of genital tuberculosis in infertile women: A comparative prospective study |
| title_full | Comparison of molecular with conventional methods for detection of genital tuberculosis in infertile women: A comparative prospective study |
| title_fullStr | Comparison of molecular with conventional methods for detection of genital tuberculosis in infertile women: A comparative prospective study |
| title_full_unstemmed | Comparison of molecular with conventional methods for detection of genital tuberculosis in infertile women: A comparative prospective study |
| title_short | Comparison of molecular with conventional methods for detection of genital tuberculosis in infertile women: A comparative prospective study |
| title_sort | comparison of molecular with conventional methods for detection of genital tuberculosis in infertile women a comparative prospective study |
| topic | tuberculosis endometritis polymerase chain reaction hysterolaparoscopy mantoux |
| url | http://jcbr.goums.ac.ir/article-1-478-en.pdf |
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