Xylanase and stimbiotic supplementation improve broilers performance and nutrient digestibility across both wheat-barley and corn-based diets

The present study investigated the effects of supplemental xylanase or stimbiotic in male broiler chickens fed either corn- or wheat-barley-based diets. A total of 1,296 Ross 308 day-old chicks were assigned to a 2 × 3 factorial design, evaluating the effects of diet grain source (wheat-barley or co...

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Main Authors: Mehdi Toghyani, Eunjoo Kim, Shemil P. Macelline, Gemma González-Ortiz, Reza Barekatain, Sonia Y. Liu
Format: Article
Language:English
Published: Elsevier 2025-07-01
Series:Poultry Science
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Online Access:http://www.sciencedirect.com/science/article/pii/S0032579125004663
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author Mehdi Toghyani
Eunjoo Kim
Shemil P. Macelline
Gemma González-Ortiz
Reza Barekatain
Sonia Y. Liu
author_facet Mehdi Toghyani
Eunjoo Kim
Shemil P. Macelline
Gemma González-Ortiz
Reza Barekatain
Sonia Y. Liu
author_sort Mehdi Toghyani
collection DOAJ
description The present study investigated the effects of supplemental xylanase or stimbiotic in male broiler chickens fed either corn- or wheat-barley-based diets. A total of 1,296 Ross 308 day-old chicks were assigned to a 2 × 3 factorial design, evaluating the effects of diet grain source (wheat-barley or corn) and additives (none, xylanase, or stimbiotic). The stimbiotic used in the present study contained xylanase and fermentable xylo-oligosaccharides. Each treatment was replicated 8 times, with 27 birds per replicate pen. At day 21, 3 birds per pen were selected for blood sample collection and another 3 birds at day 27 for digesta collection. The final body weight at day 42 was not statistically affected by grain source, additive supplementation, or their interaction (P > 0.05). Over the entire production period (0–42 d), an interaction between grain source and additive supplementation was found for feed conversion ratio (FCR), where xylanase or stimbiotic improved the FCR across the diet type, with a more pronounced improvement achieved when supplemented to the wheat-barley based diets (P < 0.01). A feed grain × additive interaction resulted in lower total feed intake in birds fed the wheat-barley based diets only in response to stimbiotic (P < 0.05). Ileal viscosity was also affected by an interaction between grain source and additive supplementation, in which viscosity reduction by xylanase or stimbiotic was only achieved in birds fed the wheat-barley based diets (P < 0.01). Similarly, an interaction was found in the ileal digestibility coefficient of protein (P = 0.016) and starch (P = 0.006), where either xylanase or stimbiotic improved the digestibility only in birds offered the wheat-barley based diets. Serum fluorescein isothiocyanate dextran level was higher in birds fed the corn-based diets compared to those fed the wheat-barley based diets (P < 0.01). These results suggest that dietary supplementation with either xylanase or stimbiotic improved feed efficiency, regardless of the dietary grain source, likely through enhanced nutrient digestibility and/or reduced digesta viscosity.
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spelling doaj-art-e796c15b8f9b4503ad2655ca9fc9ba0f2025-08-20T03:21:32ZengElsevierPoultry Science0032-57912025-07-01104710522410.1016/j.psj.2025.105224Xylanase and stimbiotic supplementation improve broilers performance and nutrient digestibility across both wheat-barley and corn-based dietsMehdi Toghyani0Eunjoo Kim1Shemil P. Macelline2Gemma González-Ortiz3Reza Barekatain4Sonia Y. Liu5School of Life and Environmental Sciences, Faculty of Science, The University of Sydney, Camperdown 2006 NSW, Australia; Poultry Research Foundation, The University of Sydney, Camden 2570 NSW, Australia; Corresponding author at: Poultry Research Foundation, The University of Sydney, Camden NSW 2570, Australia.School of Life and Environmental Sciences, Faculty of Science, The University of Sydney, Camperdown 2006 NSW, Australia; Poultry Research Foundation, The University of Sydney, Camden 2570 NSW, AustraliaSchool of Life and Environmental Sciences, Faculty of Science, The University of Sydney, Camperdown 2006 NSW, Australia; Poultry Research Foundation, The University of Sydney, Camden 2570 NSW, AustraliaAB Vista, Marlborough, Wiltshire, SN8 4AN, UKPoultry Research Foundation, The University of Sydney, Camden 2570 NSW, Australia; South Australian Research and Development Institute, Roseworthy Campus, Roseworthy, SA, AustraliaSchool of Life and Environmental Sciences, Faculty of Science, The University of Sydney, Camperdown 2006 NSW, Australia; Poultry Research Foundation, The University of Sydney, Camden 2570 NSW, AustraliaThe present study investigated the effects of supplemental xylanase or stimbiotic in male broiler chickens fed either corn- or wheat-barley-based diets. A total of 1,296 Ross 308 day-old chicks were assigned to a 2 × 3 factorial design, evaluating the effects of diet grain source (wheat-barley or corn) and additives (none, xylanase, or stimbiotic). The stimbiotic used in the present study contained xylanase and fermentable xylo-oligosaccharides. Each treatment was replicated 8 times, with 27 birds per replicate pen. At day 21, 3 birds per pen were selected for blood sample collection and another 3 birds at day 27 for digesta collection. The final body weight at day 42 was not statistically affected by grain source, additive supplementation, or their interaction (P > 0.05). Over the entire production period (0–42 d), an interaction between grain source and additive supplementation was found for feed conversion ratio (FCR), where xylanase or stimbiotic improved the FCR across the diet type, with a more pronounced improvement achieved when supplemented to the wheat-barley based diets (P < 0.01). A feed grain × additive interaction resulted in lower total feed intake in birds fed the wheat-barley based diets only in response to stimbiotic (P < 0.05). Ileal viscosity was also affected by an interaction between grain source and additive supplementation, in which viscosity reduction by xylanase or stimbiotic was only achieved in birds fed the wheat-barley based diets (P < 0.01). Similarly, an interaction was found in the ileal digestibility coefficient of protein (P = 0.016) and starch (P = 0.006), where either xylanase or stimbiotic improved the digestibility only in birds offered the wheat-barley based diets. Serum fluorescein isothiocyanate dextran level was higher in birds fed the corn-based diets compared to those fed the wheat-barley based diets (P < 0.01). These results suggest that dietary supplementation with either xylanase or stimbiotic improved feed efficiency, regardless of the dietary grain source, likely through enhanced nutrient digestibility and/or reduced digesta viscosity.http://www.sciencedirect.com/science/article/pii/S0032579125004663Grain sourceXylanaseStimbioticViscosityIntestinal permeability
spellingShingle Mehdi Toghyani
Eunjoo Kim
Shemil P. Macelline
Gemma González-Ortiz
Reza Barekatain
Sonia Y. Liu
Xylanase and stimbiotic supplementation improve broilers performance and nutrient digestibility across both wheat-barley and corn-based diets
Poultry Science
Grain source
Xylanase
Stimbiotic
Viscosity
Intestinal permeability
title Xylanase and stimbiotic supplementation improve broilers performance and nutrient digestibility across both wheat-barley and corn-based diets
title_full Xylanase and stimbiotic supplementation improve broilers performance and nutrient digestibility across both wheat-barley and corn-based diets
title_fullStr Xylanase and stimbiotic supplementation improve broilers performance and nutrient digestibility across both wheat-barley and corn-based diets
title_full_unstemmed Xylanase and stimbiotic supplementation improve broilers performance and nutrient digestibility across both wheat-barley and corn-based diets
title_short Xylanase and stimbiotic supplementation improve broilers performance and nutrient digestibility across both wheat-barley and corn-based diets
title_sort xylanase and stimbiotic supplementation improve broilers performance and nutrient digestibility across both wheat barley and corn based diets
topic Grain source
Xylanase
Stimbiotic
Viscosity
Intestinal permeability
url http://www.sciencedirect.com/science/article/pii/S0032579125004663
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