Differential Expression Characteristics of Two Isoforms <i>nr5a2f</i> and <i>nr5a2m</i> in Gonadal Differentiation of Chinese Giant Salamanders, <i>Andrias davidianus</i>
<i>Nr5a2</i> (nuclear receptor subfamily 5, group a, member 2) is involved in gonad development and sex hormone synthesis. In this study, the full length of <i>Nr5a2f</i> and <i>Nr5a2m</i> were obtained by <i>Nr5a2</i> variable splicing from <i>A...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-06-01
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| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/15/11/1667 |
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| Summary: | <i>Nr5a2</i> (nuclear receptor subfamily 5, group a, member 2) is involved in gonad development and sex hormone synthesis. In this study, the full length of <i>Nr5a2f</i> and <i>Nr5a2m</i> were obtained by <i>Nr5a2</i> variable splicing from <i>Andrias davidianus</i>, and the tissue distribution was detected. We identified <i>Nr5a2f</i> of 2455 bp and <i>Nr5a2m</i> of 2150 bp length, encoding 479 and 325 amino, respectively. We first characterized <i>Nr5a2f</i> and <i>Nr5a2m</i> gene expression in developing gonads. Results showed that <i>Nr5a2f</i> had significantly high expression in the ovary and little expression in other tissues, during the sex differentiation and sex reversal, <i>Nr5a2f</i> expression was gradually decreased in the ovary and the expression in the testis was significantly lower than in the ovary from 1 year to 6 year old. Significantly high expression was observed in the ovary and reversal ovary, while low expression was in the testis and reversal testis. While <i>Nr5a2m</i> expression exhibited the opposite profile, high expression was observed in the brain and testis. During sex differentiation and sex reversal, high expression was shown in the testis and low expression in the ovary from one year to six years old and significantly higher expression emerged in testis and reversal testis than in ovary and reversal ovary. In situ hybridization, results showed that <i>Nr5a2f</i> began to express in female undifferentiated gonads and the expression level increased from 48 dpf to 91, while <i>Nr5a2m</i> was expressed in male undifferentiated gonads. Three RNA interference sites were designed and we detected that site 293 exhibited a significant inhibitory effect in ovary cells. After <i>Nr5a2f</i> expression was inhibited by site 293, we observed that female-based gene <i>Nr5a2f, foxl2</i> and cyp19 expression were decreased, while the male-based gene <i>dmrt1</i> and <i>cyp17</i> expression was increased. These results suggested that <i>Nr5a2</i>f and <i>Nr5a2m</i> exhibited different expression patterns in the process of sex differentiation, which provided a foundation for further functional characterizations. |
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| ISSN: | 2076-2615 |