DNA Barcoding as a Tool for Surveying <i>Cytospora</i> Species Associated with Branch Dieback and Canker Diseases of Woody Plants in Canada
Background/Objectives: Branch dieback and canker diseases caused by <i>Cytospora</i> species adversely impact the health of woody plants worldwide. Results: During this survey, 59 <i>Cytospora</i> isolates were obtained from symptomatic trees and shrubs growing in southwest O...
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MDPI AG
2025-04-01
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| author | Evgeny Ilyukhin Svetlana Markovskaja |
| author_facet | Evgeny Ilyukhin Svetlana Markovskaja |
| author_sort | Evgeny Ilyukhin |
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| description | Background/Objectives: Branch dieback and canker diseases caused by <i>Cytospora</i> species adversely impact the health of woody plants worldwide. Results: During this survey, 59 <i>Cytospora</i> isolates were obtained from symptomatic trees and shrubs growing in southwest Ontario and Saskatchewan, Canada. A DNA barcoding approach combined with morphological characterization identified 15 known species of <i>Cytospora</i> associated with these diseases: <i>C. chrysosperma</i>, <i>C. curvata</i>, <i>C. euonymina</i>, <i>C. hoffmannii</i>, <i>C. kantschavelii</i>, <i>C. leucosperma</i>, <i>C. leucostoma</i>, <i>C. nitschkeana</i>, <i>C. piceae</i>, <i>C. populina</i>, <i>C. pruinopsis</i>, <i>C. pruinosa</i>, <i>C. ribis</i>, <i>C. schulzeri</i>, and <i>C. sorbina</i>. The most common species isolated from multiple hosts were <i>C. sorbina</i> (10), <i>C. chrysosperma</i> (8), <i>C. nitschkeana</i> (6), and <i>C. pruinosa</i> (6). A wide range of host associations, including non-conifer species, was observed for <i>C. piceae</i>. Conclusions: The obtained results contribute to the study of diversity, host affiliation, geographical distribution, and pathogenicity of <i>Cytospora</i> species occurring on woody plants in both natural habitats and agricultural systems. The findings support the effectiveness of using DNA barcodes in fungal taxonomy and plant pathology studies. |
| format | Article |
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| institution | Kabale University |
| issn | 2673-8856 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
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| series | DNA |
| spelling | doaj-art-e7916dfaf3754494a5c426264b105b572025-08-20T03:27:14ZengMDPI AGDNA2673-88562025-04-01522010.3390/dna5020020DNA Barcoding as a Tool for Surveying <i>Cytospora</i> Species Associated with Branch Dieback and Canker Diseases of Woody Plants in CanadaEvgeny Ilyukhin0Svetlana Markovskaja1Independent Researcher, Swift Current, SK S9H 4E6, CanadaLaboratory of Mycology, Nature Research Centre, LT 08406 Vilnius, LithuaniaBackground/Objectives: Branch dieback and canker diseases caused by <i>Cytospora</i> species adversely impact the health of woody plants worldwide. Results: During this survey, 59 <i>Cytospora</i> isolates were obtained from symptomatic trees and shrubs growing in southwest Ontario and Saskatchewan, Canada. A DNA barcoding approach combined with morphological characterization identified 15 known species of <i>Cytospora</i> associated with these diseases: <i>C. chrysosperma</i>, <i>C. curvata</i>, <i>C. euonymina</i>, <i>C. hoffmannii</i>, <i>C. kantschavelii</i>, <i>C. leucosperma</i>, <i>C. leucostoma</i>, <i>C. nitschkeana</i>, <i>C. piceae</i>, <i>C. populina</i>, <i>C. pruinopsis</i>, <i>C. pruinosa</i>, <i>C. ribis</i>, <i>C. schulzeri</i>, and <i>C. sorbina</i>. The most common species isolated from multiple hosts were <i>C. sorbina</i> (10), <i>C. chrysosperma</i> (8), <i>C. nitschkeana</i> (6), and <i>C. pruinosa</i> (6). A wide range of host associations, including non-conifer species, was observed for <i>C. piceae</i>. Conclusions: The obtained results contribute to the study of diversity, host affiliation, geographical distribution, and pathogenicity of <i>Cytospora</i> species occurring on woody plants in both natural habitats and agricultural systems. The findings support the effectiveness of using DNA barcodes in fungal taxonomy and plant pathology studies.https://www.mdpi.com/2673-8856/5/2/20branch diebackcanker disease<i>Cytospora</i>DNA barcodingpathogen |
| spellingShingle | Evgeny Ilyukhin Svetlana Markovskaja DNA Barcoding as a Tool for Surveying <i>Cytospora</i> Species Associated with Branch Dieback and Canker Diseases of Woody Plants in Canada DNA branch dieback canker disease <i>Cytospora</i> DNA barcoding pathogen |
| title | DNA Barcoding as a Tool for Surveying <i>Cytospora</i> Species Associated with Branch Dieback and Canker Diseases of Woody Plants in Canada |
| title_full | DNA Barcoding as a Tool for Surveying <i>Cytospora</i> Species Associated with Branch Dieback and Canker Diseases of Woody Plants in Canada |
| title_fullStr | DNA Barcoding as a Tool for Surveying <i>Cytospora</i> Species Associated with Branch Dieback and Canker Diseases of Woody Plants in Canada |
| title_full_unstemmed | DNA Barcoding as a Tool for Surveying <i>Cytospora</i> Species Associated with Branch Dieback and Canker Diseases of Woody Plants in Canada |
| title_short | DNA Barcoding as a Tool for Surveying <i>Cytospora</i> Species Associated with Branch Dieback and Canker Diseases of Woody Plants in Canada |
| title_sort | dna barcoding as a tool for surveying i cytospora i species associated with branch dieback and canker diseases of woody plants in canada |
| topic | branch dieback canker disease <i>Cytospora</i> DNA barcoding pathogen |
| url | https://www.mdpi.com/2673-8856/5/2/20 |
| work_keys_str_mv | AT evgenyilyukhin dnabarcodingasatoolforsurveyingicytosporaispeciesassociatedwithbranchdiebackandcankerdiseasesofwoodyplantsincanada AT svetlanamarkovskaja dnabarcodingasatoolforsurveyingicytosporaispeciesassociatedwithbranchdiebackandcankerdiseasesofwoodyplantsincanada |