DNA Barcoding as a Tool for Surveying <i>Cytospora</i> Species Associated with Branch Dieback and Canker Diseases of Woody Plants in Canada

DNA Barcoding as a Tool for Surveying <i>Cytospora</i> Species Associated with Branch Dieback and Canker Diseases of Woody Plants in Canada

Background/Objectives: Branch dieback and canker diseases caused by <i>Cytospora</i> species adversely impact the health of woody plants worldwide. Results: During this survey, 59 <i>Cytospora</i> isolates were obtained from symptomatic trees and shrubs growing in southwest O...

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Bibliographic Details
Main Authors: Evgeny Ilyukhin, Svetlana Markovskaja
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:DNA
Subjects:
branch dieback
canker disease
<i>Cytospora</i>
DNA barcoding
pathogen
Online Access:https://www.mdpi.com/2673-8856/5/2/20
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Summary:Background/Objectives: Branch dieback and canker diseases caused by <i>Cytospora</i> species adversely impact the health of woody plants worldwide. Results: During this survey, 59 <i>Cytospora</i> isolates were obtained from symptomatic trees and shrubs growing in southwest Ontario and Saskatchewan, Canada. A DNA barcoding approach combined with morphological characterization identified 15 known species of <i>Cytospora</i> associated with these diseases: <i>C. chrysosperma</i>, <i>C. curvata</i>, <i>C. euonymina</i>, <i>C. hoffmannii</i>, <i>C. kantschavelii</i>, <i>C. leucosperma</i>, <i>C. leucostoma</i>, <i>C. nitschkeana</i>, <i>C. piceae</i>, <i>C. populina</i>, <i>C. pruinopsis</i>, <i>C. pruinosa</i>, <i>C. ribis</i>, <i>C. schulzeri</i>, and <i>C. sorbina</i>. The most common species isolated from multiple hosts were <i>C. sorbina</i> (10), <i>C. chrysosperma</i> (8), <i>C. nitschkeana</i> (6), and <i>C. pruinosa</i> (6). A wide range of host associations, including non-conifer species, was observed for <i>C. piceae</i>. Conclusions: The obtained results contribute to the study of diversity, host affiliation, geographical distribution, and pathogenicity of <i>Cytospora</i> species occurring on woody plants in both natural habitats and agricultural systems. The findings support the effectiveness of using DNA barcodes in fungal taxonomy and plant pathology studies.
ISSN:2673-8856

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