In vitro anticancer and antifungal effects of Lactobacillus paracasei supernatants: a step toward sustainable food systems
Sustainability in food systems increasingly involves the integration of natural bioactive compounds that support human health while promoting eco-friendly production processes. Probiotic microorganisms, particularly those derived from food systems, have gained growing attention due to their dual rol...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-08-01
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| Series: | Frontiers in Sustainable Food Systems |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fsufs.2025.1645521/full |
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| author | Berna Erdal Bahar Yilmaz Esra Bozgeyik Seydi Yıkmış Isam A. Mohamed Ahmed Moneera O. Aljobair Monica Trif |
| author_facet | Berna Erdal Bahar Yilmaz Esra Bozgeyik Seydi Yıkmış Isam A. Mohamed Ahmed Moneera O. Aljobair Monica Trif |
| author_sort | Berna Erdal |
| collection | DOAJ |
| description | Sustainability in food systems increasingly involves the integration of natural bioactive compounds that support human health while promoting eco-friendly production processes. Probiotic microorganisms, particularly those derived from food systems, have gained growing attention due to their dual role in health promotion and sustainable biotechnological applications. This study investigates both the anticancer and antifungal properties of live and thermally inactivated cell-free supernatants (CFSs) of Lactobacillus paracasei subsp. paracasei, a food-grade probiotic, thereby addressing a significant research gap in the dual-functional potential of probiotic metabolites. Human glioma (U-87) and non-cancerous kidney epithelial (HEK293T) cells were treated with live and inactivated CFSs. A dose-dependent cytotoxic effect was observed, with IC₅₀ values indicating greater selective efficacy of the inactivated CFSs against glioma cells, while sparing normal cells. Flow cytometry and fluorescence microscopy revealed that both CFSs induced apoptosis and cell cycle arrest at the sub-G1 phase, findings further supported by DNA fragmentation analysis. In parallel, antifungal activity against Candida albicans and Candida parapsilosis was evaluated using the broth microdilution method. Notably, the inactivated CFSs exhibited potent fungistatic and fungicidal effects at lower concentrations than the live form, highlighting a novel, stable postbiotic alternative with therapeutic relevance. The present study is the first study to simultaneously demonstrate selective anticancer activity against U-87 glioma cells and broad-spectrum antifungal potential of inactivated L. paracasei CFSs. These findings represent a promising strategy for integrating probiotic-derived bioactives into sustainable food systems and functional food development. |
| format | Article |
| id | doaj-art-e7904079aeab419caf5d4bcebc764a12 |
| institution | Kabale University |
| issn | 2571-581X |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Sustainable Food Systems |
| spelling | doaj-art-e7904079aeab419caf5d4bcebc764a122025-08-20T03:39:18ZengFrontiers Media S.A.Frontiers in Sustainable Food Systems2571-581X2025-08-01910.3389/fsufs.2025.16455211645521In vitro anticancer and antifungal effects of Lactobacillus paracasei supernatants: a step toward sustainable food systemsBerna Erdal0Bahar Yilmaz1Esra Bozgeyik2Seydi Yıkmış3Isam A. Mohamed Ahmed4Moneera O. Aljobair5Monica Trif6Department of Medical Microbiology, Tekirdağ Namık Kemal University Faculty of Medicine, Tekirdağ, TürkiyeDepartment of Tumor Biology and Immunology, Institute of Health Sciences, Tekirdağ Namık Kemal Üniversitesi, Tekirdağ, TürkiyeDepartment of Medical Biology, Adiyaman University Faculty of Medicine, Adıyaman, TürkiyeDepartment of Food Technology, Tekirdağ Namık Kemal Üniversitesi, Tekirdağ, TürkiyeDepartment of Food Science and Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh, Saudi ArabiaDepartment of Sports Health, College of Sports Sciences and Physical Activity, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi ArabiaCentre for Innovative Process Engineering (CENTIV) GmbH, Syke, GermanySustainability in food systems increasingly involves the integration of natural bioactive compounds that support human health while promoting eco-friendly production processes. Probiotic microorganisms, particularly those derived from food systems, have gained growing attention due to their dual role in health promotion and sustainable biotechnological applications. This study investigates both the anticancer and antifungal properties of live and thermally inactivated cell-free supernatants (CFSs) of Lactobacillus paracasei subsp. paracasei, a food-grade probiotic, thereby addressing a significant research gap in the dual-functional potential of probiotic metabolites. Human glioma (U-87) and non-cancerous kidney epithelial (HEK293T) cells were treated with live and inactivated CFSs. A dose-dependent cytotoxic effect was observed, with IC₅₀ values indicating greater selective efficacy of the inactivated CFSs against glioma cells, while sparing normal cells. Flow cytometry and fluorescence microscopy revealed that both CFSs induced apoptosis and cell cycle arrest at the sub-G1 phase, findings further supported by DNA fragmentation analysis. In parallel, antifungal activity against Candida albicans and Candida parapsilosis was evaluated using the broth microdilution method. Notably, the inactivated CFSs exhibited potent fungistatic and fungicidal effects at lower concentrations than the live form, highlighting a novel, stable postbiotic alternative with therapeutic relevance. The present study is the first study to simultaneously demonstrate selective anticancer activity against U-87 glioma cells and broad-spectrum antifungal potential of inactivated L. paracasei CFSs. These findings represent a promising strategy for integrating probiotic-derived bioactives into sustainable food systems and functional food development.https://www.frontiersin.org/articles/10.3389/fsufs.2025.1645521/fullLactobacillus paracaseianticancerantifungalapoptosissustainable food systemspostbiotics |
| spellingShingle | Berna Erdal Bahar Yilmaz Esra Bozgeyik Seydi Yıkmış Isam A. Mohamed Ahmed Moneera O. Aljobair Monica Trif In vitro anticancer and antifungal effects of Lactobacillus paracasei supernatants: a step toward sustainable food systems Frontiers in Sustainable Food Systems Lactobacillus paracasei anticancer antifungal apoptosis sustainable food systems postbiotics |
| title | In vitro anticancer and antifungal effects of Lactobacillus paracasei supernatants: a step toward sustainable food systems |
| title_full | In vitro anticancer and antifungal effects of Lactobacillus paracasei supernatants: a step toward sustainable food systems |
| title_fullStr | In vitro anticancer and antifungal effects of Lactobacillus paracasei supernatants: a step toward sustainable food systems |
| title_full_unstemmed | In vitro anticancer and antifungal effects of Lactobacillus paracasei supernatants: a step toward sustainable food systems |
| title_short | In vitro anticancer and antifungal effects of Lactobacillus paracasei supernatants: a step toward sustainable food systems |
| title_sort | in vitro anticancer and antifungal effects of lactobacillus paracasei supernatants a step toward sustainable food systems |
| topic | Lactobacillus paracasei anticancer antifungal apoptosis sustainable food systems postbiotics |
| url | https://www.frontiersin.org/articles/10.3389/fsufs.2025.1645521/full |
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