Protective Effect of Fenugreek Supplementation against Mercury Toxicity on Sperm Parameters, Serum Testosterone and Testicular Tissue in the Rat

Background and objectives:The purpose of the current study was to investigate the effects of fenugreek supplementation against toxic effect of mercuric chloride (HgCl2)on Body weight (B.W), testis and epididymis weight, some sperm parameters, serum testosterone, serum MDA, and testis histology in th...

Full description

Saved in:
Bibliographic Details
Main Authors: Jalal A. Karim, Karim R. Hamad
Format: Article
Language:English
Published: Erbil Polytechnic University 2023-09-01
Series:Polytechnic Journal
Subjects:
Online Access: https://polytechnic-journal.epu.edu.iq/home/vol13/iss1/19
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849467629769588736
author Jalal A. Karim
Karim R. Hamad
author_facet Jalal A. Karim
Karim R. Hamad
author_sort Jalal A. Karim
collection DOAJ
description Background and objectives:The purpose of the current study was to investigate the effects of fenugreek supplementation against toxic effect of mercuric chloride (HgCl2)on Body weight (B.W), testis and epididymis weight, some sperm parameters, serum testosterone, serum MDA, and testis histology in the rat.Methods: Twenty eight male albino rats weights ranging from 254 to 302 gm and were divided randomly and equally into 4 groups. Group I: control, Group II: Rats received drinking water contained HgCl2 100 mg/L ad libitum, Group III: Rats received drinking water contained HgCl2 100 mg/L ad libitum, and fenugreek (F1) 0.75 mg/kg/day orally by gavage, Group IV: Rats received drinking water contained HgCl2 100 mg/L ad libitumand fenugreek (F2) 1.5 mg/kg/day orally by gavage. The treatments were given for six weeks.Results: Treatment with F1 caused no substantial changes in body weight, absolute weight, andrelative weight of testis and epididymis, sperm count and testosterone. Whereas sperm motility, and normal sperm were increased significantly, and serum MDA was decreased significantly from HgCl2 group. Improvement was observed partially in testis histology. Meanwhile, the protective effect in F2 generally was more than in F1.Conclusions: the present study revealed that the antioxidant activity of fenugreek supplementreduced toxic effect of mercury. In addition, steroids and/or phytoestrogens in fenugreek possibly compensated the reduced testosterone which resulted at least in part in improvement of sperm parameters and testis histology
format Article
id doaj-art-e78b1b1ef4ff40abb128abe1ba66245b
institution Kabale University
issn 2707-7799
language English
publishDate 2023-09-01
publisher Erbil Polytechnic University
record_format Article
series Polytechnic Journal
spelling doaj-art-e78b1b1ef4ff40abb128abe1ba66245b2025-08-20T03:26:09ZengErbil Polytechnic UniversityPolytechnic Journal2707-77992023-09-011317584https://doi.org/10.59341/2707-7799.1745Protective Effect of Fenugreek Supplementation against Mercury Toxicity on Sperm Parameters, Serum Testosterone and Testicular Tissue in the RatJalal A. Karim0Karim R. Hamad1Department of Biology, College of Education, Salahaddin University-Erbil, Erbil-IraqDepartment of Biology, College of Education, Salahaddin University-Erbil, Erbil-IraqBackground and objectives:The purpose of the current study was to investigate the effects of fenugreek supplementation against toxic effect of mercuric chloride (HgCl2)on Body weight (B.W), testis and epididymis weight, some sperm parameters, serum testosterone, serum MDA, and testis histology in the rat.Methods: Twenty eight male albino rats weights ranging from 254 to 302 gm and were divided randomly and equally into 4 groups. Group I: control, Group II: Rats received drinking water contained HgCl2 100 mg/L ad libitum, Group III: Rats received drinking water contained HgCl2 100 mg/L ad libitum, and fenugreek (F1) 0.75 mg/kg/day orally by gavage, Group IV: Rats received drinking water contained HgCl2 100 mg/L ad libitumand fenugreek (F2) 1.5 mg/kg/day orally by gavage. The treatments were given for six weeks.Results: Treatment with F1 caused no substantial changes in body weight, absolute weight, andrelative weight of testis and epididymis, sperm count and testosterone. Whereas sperm motility, and normal sperm were increased significantly, and serum MDA was decreased significantly from HgCl2 group. Improvement was observed partially in testis histology. Meanwhile, the protective effect in F2 generally was more than in F1.Conclusions: the present study revealed that the antioxidant activity of fenugreek supplementreduced toxic effect of mercury. In addition, steroids and/or phytoestrogens in fenugreek possibly compensated the reduced testosterone which resulted at least in part in improvement of sperm parameters and testis histology https://polytechnic-journal.epu.edu.iq/home/vol13/iss1/19 fenugreek;mercury;sperm;testis; mda;testosterone
spellingShingle Jalal A. Karim
Karim R. Hamad
Protective Effect of Fenugreek Supplementation against Mercury Toxicity on Sperm Parameters, Serum Testosterone and Testicular Tissue in the Rat
Polytechnic Journal
fenugreek;
mercury;
sperm;
testis; mda;
testosterone
title Protective Effect of Fenugreek Supplementation against Mercury Toxicity on Sperm Parameters, Serum Testosterone and Testicular Tissue in the Rat
title_full Protective Effect of Fenugreek Supplementation against Mercury Toxicity on Sperm Parameters, Serum Testosterone and Testicular Tissue in the Rat
title_fullStr Protective Effect of Fenugreek Supplementation against Mercury Toxicity on Sperm Parameters, Serum Testosterone and Testicular Tissue in the Rat
title_full_unstemmed Protective Effect of Fenugreek Supplementation against Mercury Toxicity on Sperm Parameters, Serum Testosterone and Testicular Tissue in the Rat
title_short Protective Effect of Fenugreek Supplementation against Mercury Toxicity on Sperm Parameters, Serum Testosterone and Testicular Tissue in the Rat
title_sort protective effect of fenugreek supplementation against mercury toxicity on sperm parameters serum testosterone and testicular tissue in the rat
topic fenugreek;
mercury;
sperm;
testis; mda;
testosterone
url https://polytechnic-journal.epu.edu.iq/home/vol13/iss1/19
work_keys_str_mv AT jalalakarim protectiveeffectoffenugreeksupplementationagainstmercurytoxicityonspermparametersserumtestosteroneandtesticulartissueintherat
AT karimrhamad protectiveeffectoffenugreeksupplementationagainstmercurytoxicityonspermparametersserumtestosteroneandtesticulartissueintherat