CD11c-Specific Deletion Reveals CREB as a Critical Regulator of DC Function during the Germinal Center Response
Dendritic cells (DCs) are crucial for the balance between immune response and tolerance, but the molecular mechanism regulating development, differentiation, and homeostasis are poorly understood. The transcriptional activator CREB is involved in regulating different cells of the innate and adaptive...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-01-01
|
| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2018/8947230 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849467555310206976 |
|---|---|
| author | Kim Ohl Anastasia Schippers Klaus Tenbrock |
| author_facet | Kim Ohl Anastasia Schippers Klaus Tenbrock |
| author_sort | Kim Ohl |
| collection | DOAJ |
| description | Dendritic cells (DCs) are crucial for the balance between immune response and tolerance, but the molecular mechanism regulating development, differentiation, and homeostasis are poorly understood. The transcriptional activator CREB is involved in regulating different cells of the innate and adaptive immune system and is a transcriptional regulator of development, survival, activation, or proliferation in macrophages, dendritic cells, B cells, and T cells. To directly examine the role of CREB in the regulation of DCs, the CREB gene was targeted for deletion with a CD11c-cre transgene. The deletion of CREB in CD11c+ cells did not involve any developmental or systemic defects within DC populations. However, CREB deficiency in CD11c+ cells reduced germinal center (GC) B cells in steady state, and immunization with NP-CGG resulted in a reduced formation of GCs, paralleled by the reduced production of IgGs in sera of immunized mice. In conclusion, we demonstrate that CREB expression in CD11c+ cells enhances germinal center responses, most likely by altering DC function, which might have implications for autoimmune diseases that are associated with dysregulated GC responses. |
| format | Article |
| id | doaj-art-e786967b12404c9e80fd8541e28098db |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-e786967b12404c9e80fd8541e28098db2025-08-20T03:26:10ZengWileyJournal of Immunology Research2314-88612314-71562018-01-01201810.1155/2018/89472308947230CD11c-Specific Deletion Reveals CREB as a Critical Regulator of DC Function during the Germinal Center ResponseKim Ohl0Anastasia Schippers1Klaus Tenbrock2Department of Pediatrics, Medical Faculty, RWTH Aachen, Aachen, GermanyDepartment of Pediatrics, Medical Faculty, RWTH Aachen, Aachen, GermanyDepartment of Pediatrics, Medical Faculty, RWTH Aachen, Aachen, GermanyDendritic cells (DCs) are crucial for the balance between immune response and tolerance, but the molecular mechanism regulating development, differentiation, and homeostasis are poorly understood. The transcriptional activator CREB is involved in regulating different cells of the innate and adaptive immune system and is a transcriptional regulator of development, survival, activation, or proliferation in macrophages, dendritic cells, B cells, and T cells. To directly examine the role of CREB in the regulation of DCs, the CREB gene was targeted for deletion with a CD11c-cre transgene. The deletion of CREB in CD11c+ cells did not involve any developmental or systemic defects within DC populations. However, CREB deficiency in CD11c+ cells reduced germinal center (GC) B cells in steady state, and immunization with NP-CGG resulted in a reduced formation of GCs, paralleled by the reduced production of IgGs in sera of immunized mice. In conclusion, we demonstrate that CREB expression in CD11c+ cells enhances germinal center responses, most likely by altering DC function, which might have implications for autoimmune diseases that are associated with dysregulated GC responses.http://dx.doi.org/10.1155/2018/8947230 |
| spellingShingle | Kim Ohl Anastasia Schippers Klaus Tenbrock CD11c-Specific Deletion Reveals CREB as a Critical Regulator of DC Function during the Germinal Center Response Journal of Immunology Research |
| title | CD11c-Specific Deletion Reveals CREB as a Critical Regulator of DC Function during the Germinal Center Response |
| title_full | CD11c-Specific Deletion Reveals CREB as a Critical Regulator of DC Function during the Germinal Center Response |
| title_fullStr | CD11c-Specific Deletion Reveals CREB as a Critical Regulator of DC Function during the Germinal Center Response |
| title_full_unstemmed | CD11c-Specific Deletion Reveals CREB as a Critical Regulator of DC Function during the Germinal Center Response |
| title_short | CD11c-Specific Deletion Reveals CREB as a Critical Regulator of DC Function during the Germinal Center Response |
| title_sort | cd11c specific deletion reveals creb as a critical regulator of dc function during the germinal center response |
| url | http://dx.doi.org/10.1155/2018/8947230 |
| work_keys_str_mv | AT kimohl cd11cspecificdeletionrevealscrebasacriticalregulatorofdcfunctionduringthegerminalcenterresponse AT anastasiaschippers cd11cspecificdeletionrevealscrebasacriticalregulatorofdcfunctionduringthegerminalcenterresponse AT klaustenbrock cd11cspecificdeletionrevealscrebasacriticalregulatorofdcfunctionduringthegerminalcenterresponse |