Imipramine-induced immunomodulation and intracellular growth inhibition during Brucella abortus 544 infection in RAW 264.7 cells and BALB/c mice
Brucellosis is a significant zoonotic infection with increasing global prevalence. Traditional treatments rely on antibiotic combinations, but challenges such as drug resistance and relapse necessitate the exploration of alternative therapeutic options. Imipramine hydrochloride (ImiP) has shown pote...
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Frontiers Media S.A.
2025-06-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2025.1598106/full |
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| author | Ched Nicole Turbela Aguilar Tran Xuan Ngoc Huy Trang Thi Nguyen Said Abdi Salad Seong Eun Cho Il-Hwa Hong Wongi Min Hu Jang Lee Suk Kim |
| author_facet | Ched Nicole Turbela Aguilar Tran Xuan Ngoc Huy Trang Thi Nguyen Said Abdi Salad Seong Eun Cho Il-Hwa Hong Wongi Min Hu Jang Lee Suk Kim |
| author_sort | Ched Nicole Turbela Aguilar |
| collection | DOAJ |
| description | Brucellosis is a significant zoonotic infection with increasing global prevalence. Traditional treatments rely on antibiotic combinations, but challenges such as drug resistance and relapse necessitate the exploration of alternative therapeutic options. Imipramine hydrochloride (ImiP) has shown potential as an adjunctive treatment for infectious diseases. This study investigates the immunomodulatory effects of ImiP in B. abortus 544 infections in murine macrophages and BALB/c mice. In vitro, RAW 264.7 cells exposed to ImiP exhibited reduced B. abortus replication, decreased nitrite levels, and enhanced bactericidal effects. In vivo, ImiP treatment significantly decreased bacterial loads in the spleen (10 mg/kg, **p < 0.01; 20 mg/kg, *p < 0.05) and liver (10 mg/kg, **p < 0.01; 20 mg/kg, ***p < 0.001), compared to untreated controls. Histopathological analysis revealed minimal liver microgranuloma formation and periportal inflammation in ImiP-treated mice. Moreover, flow cytometry showed decreased CD4+ and CD8+ T cell expression, while serum cytokine profiling indicated a Th1-driven immune response, characterized by elevated levels of IL-12 and decreased IL-10. These findings suggest that ImiP possesses both immunomodulatory and antibacterial effects, highlighting its potential as an adjunctive therapy for brucellosis. |
| format | Article |
| id | doaj-art-e7867bb7d47b407e94594891ea935238 |
| institution | DOAJ |
| issn | 2297-1769 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj-art-e7867bb7d47b407e94594891ea9352382025-08-20T03:20:10ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-06-011210.3389/fvets.2025.15981061598106Imipramine-induced immunomodulation and intracellular growth inhibition during Brucella abortus 544 infection in RAW 264.7 cells and BALB/c miceChed Nicole Turbela Aguilar0Tran Xuan Ngoc Huy1Trang Thi Nguyen2Said Abdi Salad3Seong Eun Cho4Il-Hwa Hong5Wongi Min6Hu Jang Lee7Suk Kim8Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of KoreaInstitute of Applied Sciences, HUTECH University, Ho Chi Minh City, VietnamInstitute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of KoreaInstitute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of KoreaInstitute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of KoreaInstitute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of KoreaInstitute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of KoreaInstitute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of KoreaInstitute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of KoreaBrucellosis is a significant zoonotic infection with increasing global prevalence. Traditional treatments rely on antibiotic combinations, but challenges such as drug resistance and relapse necessitate the exploration of alternative therapeutic options. Imipramine hydrochloride (ImiP) has shown potential as an adjunctive treatment for infectious diseases. This study investigates the immunomodulatory effects of ImiP in B. abortus 544 infections in murine macrophages and BALB/c mice. In vitro, RAW 264.7 cells exposed to ImiP exhibited reduced B. abortus replication, decreased nitrite levels, and enhanced bactericidal effects. In vivo, ImiP treatment significantly decreased bacterial loads in the spleen (10 mg/kg, **p < 0.01; 20 mg/kg, *p < 0.05) and liver (10 mg/kg, **p < 0.01; 20 mg/kg, ***p < 0.001), compared to untreated controls. Histopathological analysis revealed minimal liver microgranuloma formation and periportal inflammation in ImiP-treated mice. Moreover, flow cytometry showed decreased CD4+ and CD8+ T cell expression, while serum cytokine profiling indicated a Th1-driven immune response, characterized by elevated levels of IL-12 and decreased IL-10. These findings suggest that ImiP possesses both immunomodulatory and antibacterial effects, highlighting its potential as an adjunctive therapy for brucellosis.https://www.frontiersin.org/articles/10.3389/fvets.2025.1598106/fullBrucella abortusimipramine hydrochlorideBALB/c mouseRAW 264.7 cellsimmunomodulation |
| spellingShingle | Ched Nicole Turbela Aguilar Tran Xuan Ngoc Huy Trang Thi Nguyen Said Abdi Salad Seong Eun Cho Il-Hwa Hong Wongi Min Hu Jang Lee Suk Kim Imipramine-induced immunomodulation and intracellular growth inhibition during Brucella abortus 544 infection in RAW 264.7 cells and BALB/c mice Frontiers in Veterinary Science Brucella abortus imipramine hydrochloride BALB/c mouse RAW 264.7 cells immunomodulation |
| title | Imipramine-induced immunomodulation and intracellular growth inhibition during Brucella abortus 544 infection in RAW 264.7 cells and BALB/c mice |
| title_full | Imipramine-induced immunomodulation and intracellular growth inhibition during Brucella abortus 544 infection in RAW 264.7 cells and BALB/c mice |
| title_fullStr | Imipramine-induced immunomodulation and intracellular growth inhibition during Brucella abortus 544 infection in RAW 264.7 cells and BALB/c mice |
| title_full_unstemmed | Imipramine-induced immunomodulation and intracellular growth inhibition during Brucella abortus 544 infection in RAW 264.7 cells and BALB/c mice |
| title_short | Imipramine-induced immunomodulation and intracellular growth inhibition during Brucella abortus 544 infection in RAW 264.7 cells and BALB/c mice |
| title_sort | imipramine induced immunomodulation and intracellular growth inhibition during brucella abortus 544 infection in raw 264 7 cells and balb c mice |
| topic | Brucella abortus imipramine hydrochloride BALB/c mouse RAW 264.7 cells immunomodulation |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2025.1598106/full |
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