Virulence Profiles, Phylogenetic Background, and Antibiotic Resistance of Escherichia coli Isolated from Turkeys with Airsacculitis
Avian Pathogenic Escherichia coli (APEC) has been studied for decades because of its economic impact on the poultry industry. Recently, the zoonotic potential of APEC and multidrug-resistant strains have emerged. The aim of this study was to characterize 225 APEC isolated from turkeys presenting air...
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/289024 |
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| author | Marcos Paulo Vieira Cunha Maria Gabriela Xavier de Oliveira Mirela Caroline Vilela de Oliveira Ketrin Cristina da Silva Cleise Ribeiro Gomes Andrea Micke Moreno Terezinha Knöbl |
| author_facet | Marcos Paulo Vieira Cunha Maria Gabriela Xavier de Oliveira Mirela Caroline Vilela de Oliveira Ketrin Cristina da Silva Cleise Ribeiro Gomes Andrea Micke Moreno Terezinha Knöbl |
| author_sort | Marcos Paulo Vieira Cunha |
| collection | DOAJ |
| description | Avian Pathogenic Escherichia coli (APEC) has been studied for decades because of its economic impact on the poultry industry. Recently, the zoonotic potential of APEC and multidrug-resistant strains have emerged. The aim of this study was to characterize 225 APEC isolated from turkeys presenting airsacculitis. The results showed that 92% of strains presented a multidrug-resistance (MDR), and the highest levels of resistance were to sulfamethazine (94%) and tetracycline (83%). Half of these strains were classified in phylogenetic group B2, followed by B1 (28.6%), A (17.1%), and D (4.8%). The prevalence of virulence genes was as follows: salmochelin (iroN, 95%), increased serum survival (iss, 93%), colicin V (cvi/cva, 67%), aerobactin (iucD, 67%), temperature-sensitive haemagglutinin (tsh, 56%), iron-repressible protein (irp2, 51%), invasion brain endothelium (ibeA, 31%), vacuolating autotransporter toxin (vat, 24%), K1 antigen (neuS, 19%), enteroaggregative heat-stable cytotoxin (astA, 17%), and pilus associated with pyelonephritis (papC, 15%). These results demonstrate that the majority of the investigated strains belonged to group B2 and were MDR. These data suggest that turkeys may serve as a reservoir of pathogenic and multidrug-resistance strains, reinforcing the idea that poultry plays a role in the epidemiological chain of ExPEC. |
| format | Article |
| id | doaj-art-e78333a98e1c40f6aa923d1b019ce328 |
| institution | OA Journals |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-e78333a98e1c40f6aa923d1b019ce3282025-08-20T02:21:10ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/289024289024Virulence Profiles, Phylogenetic Background, and Antibiotic Resistance of Escherichia coli Isolated from Turkeys with AirsacculitisMarcos Paulo Vieira Cunha0Maria Gabriela Xavier de Oliveira1Mirela Caroline Vilela de Oliveira2Ketrin Cristina da Silva3Cleise Ribeiro Gomes4Andrea Micke Moreno5Terezinha Knöbl6Departamento de Patologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Avenida Professor Orlando Marques de Paiva, No. 87, Cidade Universitária, 05508-270 São Paulo, SP, BrazilDepartamento de Patologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Avenida Professor Orlando Marques de Paiva, No. 87, Cidade Universitária, 05508-270 São Paulo, SP, BrazilDepartamento de Patologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Avenida Professor Orlando Marques de Paiva, No. 87, Cidade Universitária, 05508-270 São Paulo, SP, BrazilDepartamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Avenida Professor Orlando Marques de Paiva, No. 87, Cidade Universitária, 05508-270 São Paulo, SP, BrazilDepartamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Avenida Professor Orlando Marques de Paiva, No. 87, Cidade Universitária, 05508-270 São Paulo, SP, BrazilDepartamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Avenida Professor Orlando Marques de Paiva, No. 87, Cidade Universitária, 05508-270 São Paulo, SP, BrazilDepartamento de Patologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Avenida Professor Orlando Marques de Paiva, No. 87, Cidade Universitária, 05508-270 São Paulo, SP, BrazilAvian Pathogenic Escherichia coli (APEC) has been studied for decades because of its economic impact on the poultry industry. Recently, the zoonotic potential of APEC and multidrug-resistant strains have emerged. The aim of this study was to characterize 225 APEC isolated from turkeys presenting airsacculitis. The results showed that 92% of strains presented a multidrug-resistance (MDR), and the highest levels of resistance were to sulfamethazine (94%) and tetracycline (83%). Half of these strains were classified in phylogenetic group B2, followed by B1 (28.6%), A (17.1%), and D (4.8%). The prevalence of virulence genes was as follows: salmochelin (iroN, 95%), increased serum survival (iss, 93%), colicin V (cvi/cva, 67%), aerobactin (iucD, 67%), temperature-sensitive haemagglutinin (tsh, 56%), iron-repressible protein (irp2, 51%), invasion brain endothelium (ibeA, 31%), vacuolating autotransporter toxin (vat, 24%), K1 antigen (neuS, 19%), enteroaggregative heat-stable cytotoxin (astA, 17%), and pilus associated with pyelonephritis (papC, 15%). These results demonstrate that the majority of the investigated strains belonged to group B2 and were MDR. These data suggest that turkeys may serve as a reservoir of pathogenic and multidrug-resistance strains, reinforcing the idea that poultry plays a role in the epidemiological chain of ExPEC.http://dx.doi.org/10.1155/2014/289024 |
| spellingShingle | Marcos Paulo Vieira Cunha Maria Gabriela Xavier de Oliveira Mirela Caroline Vilela de Oliveira Ketrin Cristina da Silva Cleise Ribeiro Gomes Andrea Micke Moreno Terezinha Knöbl Virulence Profiles, Phylogenetic Background, and Antibiotic Resistance of Escherichia coli Isolated from Turkeys with Airsacculitis The Scientific World Journal |
| title | Virulence Profiles, Phylogenetic Background, and Antibiotic Resistance of Escherichia coli Isolated from Turkeys with Airsacculitis |
| title_full | Virulence Profiles, Phylogenetic Background, and Antibiotic Resistance of Escherichia coli Isolated from Turkeys with Airsacculitis |
| title_fullStr | Virulence Profiles, Phylogenetic Background, and Antibiotic Resistance of Escherichia coli Isolated from Turkeys with Airsacculitis |
| title_full_unstemmed | Virulence Profiles, Phylogenetic Background, and Antibiotic Resistance of Escherichia coli Isolated from Turkeys with Airsacculitis |
| title_short | Virulence Profiles, Phylogenetic Background, and Antibiotic Resistance of Escherichia coli Isolated from Turkeys with Airsacculitis |
| title_sort | virulence profiles phylogenetic background and antibiotic resistance of escherichia coli isolated from turkeys with airsacculitis |
| url | http://dx.doi.org/10.1155/2014/289024 |
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