Change of reception and lactate transport by cerebral endothelium under the influence of viral and bacterial inflammation in vitro
Aim. To study the effect of neuroinflammation inducers of viral and bacterial origin on the reception and transport of lactate in endothelial cells of the brain in vitro.Materials and Methods. In the study, we incubated endothelial cells with bacterial inducers (lipopolysaccharide, E. coli) of vario...
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| Format: | Article |
| Language: | Russian |
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Kemerovo State Medical University
2020-03-01
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| Series: | Фундаментальная и клиническая медицина |
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| Online Access: | https://fcm.kemsmu.ru/jour/article/view/215 |
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| author | E. B. Boytsova A. V. Morgun E. D. Osipova G. P. Martinova A. B. Salmina |
| author_facet | E. B. Boytsova A. V. Morgun E. D. Osipova G. P. Martinova A. B. Salmina |
| author_sort | E. B. Boytsova |
| collection | DOAJ |
| description | Aim. To study the effect of neuroinflammation inducers of viral and bacterial origin on the reception and transport of lactate in endothelial cells of the brain in vitro.Materials and Methods. In the study, we incubated endothelial cells with bacterial inducers (lipopolysaccharide, E. coli) of various concentrations and inducer of viral inflammation (Poly I:C). After the exposure, we evaluated the proliferative activity of endothelial cells, change in transendothelial electrical resistance, and the concentration of extracellular lactate. We then performed an im-munocytochemistry investigation to record changes in the number of endothelial cells expressing the target molecules GPR81, MCT-1, IL-1β, TLR3 and ZO1.Conclusions. Disturbance of the transport and reception of lactate can contribute to the changes in structural and functional integrity of the blood-brain barrier caused by bacterial and viral inflammation. |
| format | Article |
| id | doaj-art-e77b04818eba408f8f180191e0aadfb4 |
| institution | DOAJ |
| issn | 2500-0764 2542-0941 |
| language | Russian |
| publishDate | 2020-03-01 |
| publisher | Kemerovo State Medical University |
| record_format | Article |
| series | Фундаментальная и клиническая медицина |
| spelling | doaj-art-e77b04818eba408f8f180191e0aadfb42025-08-20T02:49:12ZrusKemerovo State Medical UniversityФундаментальная и клиническая медицина2500-07642542-09412020-03-015181410.23946/2500-0764-2020-5-1-8-14184Change of reception and lactate transport by cerebral endothelium under the influence of viral and bacterial inflammation in vitroE. B. Boytsova0A. V. Morgun1E. D. Osipova2G. P. Martinova3A. B. Salmina4Voino-Yasenetskiy Krasnoyarsk State Medical UniversityVoino-Yasenetskiy Krasnoyarsk State Medical UniversityVoino-Yasenetskiy Krasnoyarsk State Medical UniversityVoino-Yasenetskiy Krasnoyarsk State Medical UniversityVoino-Yasenetskiy Krasnoyarsk State Medical UniversityAim. To study the effect of neuroinflammation inducers of viral and bacterial origin on the reception and transport of lactate in endothelial cells of the brain in vitro.Materials and Methods. In the study, we incubated endothelial cells with bacterial inducers (lipopolysaccharide, E. coli) of various concentrations and inducer of viral inflammation (Poly I:C). After the exposure, we evaluated the proliferative activity of endothelial cells, change in transendothelial electrical resistance, and the concentration of extracellular lactate. We then performed an im-munocytochemistry investigation to record changes in the number of endothelial cells expressing the target molecules GPR81, MCT-1, IL-1β, TLR3 and ZO1.Conclusions. Disturbance of the transport and reception of lactate can contribute to the changes in structural and functional integrity of the blood-brain barrier caused by bacterial and viral inflammation.https://fcm.kemsmu.ru/jour/article/view/215lipopolysaccharideneuroinflammationblood-brain barrierlactatepolyi:c |
| spellingShingle | E. B. Boytsova A. V. Morgun E. D. Osipova G. P. Martinova A. B. Salmina Change of reception and lactate transport by cerebral endothelium under the influence of viral and bacterial inflammation in vitro Фундаментальная и клиническая медицина lipopolysaccharide neuroinflammation blood-brain barrier lactate polyi:c |
| title | Change of reception and lactate transport by cerebral endothelium under the influence of viral and bacterial inflammation in vitro |
| title_full | Change of reception and lactate transport by cerebral endothelium under the influence of viral and bacterial inflammation in vitro |
| title_fullStr | Change of reception and lactate transport by cerebral endothelium under the influence of viral and bacterial inflammation in vitro |
| title_full_unstemmed | Change of reception and lactate transport by cerebral endothelium under the influence of viral and bacterial inflammation in vitro |
| title_short | Change of reception and lactate transport by cerebral endothelium under the influence of viral and bacterial inflammation in vitro |
| title_sort | change of reception and lactate transport by cerebral endothelium under the influence of viral and bacterial inflammation in vitro |
| topic | lipopolysaccharide neuroinflammation blood-brain barrier lactate polyi:c |
| url | https://fcm.kemsmu.ru/jour/article/view/215 |
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