The unique hypertrophic and fibrotic features of neonatal right ventricle in response to pressure overload
Abstract Pediatric heart failure (HF) research remains in its infancy partly due to the lack of neonatal rat/mouse models of HF. The aim of the study is to introduce a neonatal rat/mouse model of right ventricular (RV) pressure overload (RVPO), a significant cause of pediatric HF, and to uncover the...
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Nature Portfolio
2025-05-01
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| Online Access: | https://doi.org/10.1038/s41598-025-01427-y |
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| author | Yingying Xiao Yiting Xue Debao Li Lincai Ye Zheng Wang Sixie Zheng Peisen Ruan Hao Chen Haifa Hong |
| author_facet | Yingying Xiao Yiting Xue Debao Li Lincai Ye Zheng Wang Sixie Zheng Peisen Ruan Hao Chen Haifa Hong |
| author_sort | Yingying Xiao |
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| description | Abstract Pediatric heart failure (HF) research remains in its infancy partly due to the lack of neonatal rat/mouse models of HF. The aim of the study is to introduce a neonatal rat/mouse model of right ventricular (RV) pressure overload (RVPO), a significant cause of pediatric HF, and to uncover the molecular features of RVPO-induced RV hypertrophy and fibrosis—the two most important transitional pathological states between normal and dysfunctional RV. Neonatal rat/mouse model of RVPO was established by pulmonary artery banding (PAB) surgery on postnatal day 1(P1) and confirmed by echocardiography and morphological examination on P7. Bulk RNA and single-cell RNA sequencing was performed on RV tissues, along with bulk RNA sequencing on RV cardiomyocytes, to screen a range of key genes and signaling pathways that are upregulated and that play critical roles in adult hypertrophy and fibrosis. The sequencing results were further verified by qRT-PCR and histological staining. Most of the pathways and associated genes, such as oxidative stress, inflammation, phosphodiesterase, proteasome, protein kinase, transforming growth factor, and angiotensin were not changed or downregulated in the neonatal RVPO model. This study reveals the unique features of hypertrophy and fibrosis in the neonatal RV in response to pressure overload, which partly explains why adult-effective anti-HF drugs fail to treat pediatric HF. More importantly, single-cell RNA sequencing data of the neonatal RV with pressure overload were documented, providing an important reference for future basic or clinical investigations on pediatric RV failure. |
| format | Article |
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| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-05-01 |
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| spelling | doaj-art-e77a68fcabd54816b157b1ce962b60972025-08-20T02:34:17ZengNature PortfolioScientific Reports2045-23222025-05-0115111410.1038/s41598-025-01427-yThe unique hypertrophic and fibrotic features of neonatal right ventricle in response to pressure overloadYingying Xiao0Yiting Xue1Debao Li2Lincai Ye3Zheng Wang4Sixie Zheng5Peisen Ruan6Hao Chen7Haifa Hong8Department of Thoracic and Cardiovascular Surgery, Shanghai Children’s Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Thoracic and Cardiovascular Surgery, Shanghai Children’s Medical Center, Shanghai Jiao Tong University School of MedicineDepartment of Pediatric Surgery, Children’s Hospital of Fudan University, National Children’s Medical CenterDepartment of Thoracic and Cardiovascular Surgery, Shanghai Children’s Medical Center, Shanghai Jiao Tong University School of MedicineDepartment of Thoracic and Cardiovascular Surgery, Shanghai Children’s Medical Center, Shanghai Jiao Tong University School of MedicineDepartment of Thoracic and Cardiovascular Surgery, Shanghai Children’s Medical Center, Shanghai Jiao Tong University School of MedicineDepartment of Critical Care Medicine, The Affiliated Women and Children’s Hospital of Ningbo UniversityDepartment of Thoracic and Cardiovascular Surgery, Shanghai Children’s Medical Center, Shanghai Jiao Tong University School of MedicineDepartment of Thoracic and Cardiovascular Surgery, Shanghai Children’s Hospital, Shanghai Jiao Tong University School of MedicineAbstract Pediatric heart failure (HF) research remains in its infancy partly due to the lack of neonatal rat/mouse models of HF. The aim of the study is to introduce a neonatal rat/mouse model of right ventricular (RV) pressure overload (RVPO), a significant cause of pediatric HF, and to uncover the molecular features of RVPO-induced RV hypertrophy and fibrosis—the two most important transitional pathological states between normal and dysfunctional RV. Neonatal rat/mouse model of RVPO was established by pulmonary artery banding (PAB) surgery on postnatal day 1(P1) and confirmed by echocardiography and morphological examination on P7. Bulk RNA and single-cell RNA sequencing was performed on RV tissues, along with bulk RNA sequencing on RV cardiomyocytes, to screen a range of key genes and signaling pathways that are upregulated and that play critical roles in adult hypertrophy and fibrosis. The sequencing results were further verified by qRT-PCR and histological staining. Most of the pathways and associated genes, such as oxidative stress, inflammation, phosphodiesterase, proteasome, protein kinase, transforming growth factor, and angiotensin were not changed or downregulated in the neonatal RVPO model. This study reveals the unique features of hypertrophy and fibrosis in the neonatal RV in response to pressure overload, which partly explains why adult-effective anti-HF drugs fail to treat pediatric HF. More importantly, single-cell RNA sequencing data of the neonatal RV with pressure overload were documented, providing an important reference for future basic or clinical investigations on pediatric RV failure.https://doi.org/10.1038/s41598-025-01427-yPressure overloadRight heart failurePediatricSingle-cell sequencingRight ventricle |
| spellingShingle | Yingying Xiao Yiting Xue Debao Li Lincai Ye Zheng Wang Sixie Zheng Peisen Ruan Hao Chen Haifa Hong The unique hypertrophic and fibrotic features of neonatal right ventricle in response to pressure overload Scientific Reports Pressure overload Right heart failure Pediatric Single-cell sequencing Right ventricle |
| title | The unique hypertrophic and fibrotic features of neonatal right ventricle in response to pressure overload |
| title_full | The unique hypertrophic and fibrotic features of neonatal right ventricle in response to pressure overload |
| title_fullStr | The unique hypertrophic and fibrotic features of neonatal right ventricle in response to pressure overload |
| title_full_unstemmed | The unique hypertrophic and fibrotic features of neonatal right ventricle in response to pressure overload |
| title_short | The unique hypertrophic and fibrotic features of neonatal right ventricle in response to pressure overload |
| title_sort | unique hypertrophic and fibrotic features of neonatal right ventricle in response to pressure overload |
| topic | Pressure overload Right heart failure Pediatric Single-cell sequencing Right ventricle |
| url | https://doi.org/10.1038/s41598-025-01427-y |
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