Breast cancer brain metastasis: evaluating the effectiveness of alginate-based organoids in metastasis modeling to replace matrigel
Abstract Background One of the most important and devastating side effects of breast cancer is brain metastasis. Our understanding of cancer heterogeneity is revolutionized by tumoral organoids and seems promising for personalized medicine. This study aimed to generate a hydrogel-based brain metasta...
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BMC
2025-03-01
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| Series: | BMC Research Notes |
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| Online Access: | https://doi.org/10.1186/s13104-025-07154-x |
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| author | Sepehr Zamani Fateme Sadat Bitaraf Mohammad Kamalabadi-Farahani |
| author_facet | Sepehr Zamani Fateme Sadat Bitaraf Mohammad Kamalabadi-Farahani |
| author_sort | Sepehr Zamani |
| collection | DOAJ |
| description | Abstract Background One of the most important and devastating side effects of breast cancer is brain metastasis. Our understanding of cancer heterogeneity is revolutionized by tumoral organoids and seems promising for personalized medicine. This study aimed to generate a hydrogel-based brain metastasis organoid. Methods Mouse brain metastatic tumor cells (4T1B) were isolated and cultured from the brain metastasis lesions of mice with breast cancer. Different hydrogels, including alginate, carboxymethylcellulose, gelatin, collagen, and matrigel, were prepared. Pre-coated hydrogels in 96-well plates were treated with 4T1B cells. The morphology and viability of metastatic organoids were analyzed after 7 days. Results According to our results, 4T1B cells formed semi-regular cluster structures in alginate hydrogel. In this group, the cell survival rate and formation of three-dimensional structures were significantly higher than in other groups. Conclusion For organoid cultures, there’s a lot of research on natural and synthetic scaffolds that are chemically or mechanically well-designed. In the present study, we used highly brain metastatic tumor cells and detected that alginate hydrogel is the best choice for organoid formation and breast cancer brain metastasis modeling. |
| format | Article |
| id | doaj-art-e7778d052bbd45aca7864b2e2f8434d8 |
| institution | OA Journals |
| issn | 1756-0500 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj-art-e7778d052bbd45aca7864b2e2f8434d82025-08-20T01:52:59ZengBMCBMC Research Notes1756-05002025-03-011811710.1186/s13104-025-07154-xBreast cancer brain metastasis: evaluating the effectiveness of alginate-based organoids in metastasis modeling to replace matrigelSepehr Zamani0Fateme Sadat Bitaraf1Mohammad Kamalabadi-Farahani2Student Research Committee, School of Medicine, Shahroud University of Medical SciencesDepartment of Medical Biotechnology, School of Medicine, Shahroud University of Medical SciencesDepartment of Tissue Engineering, School of Medicine, Shahroud University of Medical SciencesAbstract Background One of the most important and devastating side effects of breast cancer is brain metastasis. Our understanding of cancer heterogeneity is revolutionized by tumoral organoids and seems promising for personalized medicine. This study aimed to generate a hydrogel-based brain metastasis organoid. Methods Mouse brain metastatic tumor cells (4T1B) were isolated and cultured from the brain metastasis lesions of mice with breast cancer. Different hydrogels, including alginate, carboxymethylcellulose, gelatin, collagen, and matrigel, were prepared. Pre-coated hydrogels in 96-well plates were treated with 4T1B cells. The morphology and viability of metastatic organoids were analyzed after 7 days. Results According to our results, 4T1B cells formed semi-regular cluster structures in alginate hydrogel. In this group, the cell survival rate and formation of three-dimensional structures were significantly higher than in other groups. Conclusion For organoid cultures, there’s a lot of research on natural and synthetic scaffolds that are chemically or mechanically well-designed. In the present study, we used highly brain metastatic tumor cells and detected that alginate hydrogel is the best choice for organoid formation and breast cancer brain metastasis modeling.https://doi.org/10.1186/s13104-025-07154-xOrganogenesisBiomaterialsNatural polymersBreast tumoBrain neoplasm |
| spellingShingle | Sepehr Zamani Fateme Sadat Bitaraf Mohammad Kamalabadi-Farahani Breast cancer brain metastasis: evaluating the effectiveness of alginate-based organoids in metastasis modeling to replace matrigel BMC Research Notes Organogenesis Biomaterials Natural polymers Breast tumo Brain neoplasm |
| title | Breast cancer brain metastasis: evaluating the effectiveness of alginate-based organoids in metastasis modeling to replace matrigel |
| title_full | Breast cancer brain metastasis: evaluating the effectiveness of alginate-based organoids in metastasis modeling to replace matrigel |
| title_fullStr | Breast cancer brain metastasis: evaluating the effectiveness of alginate-based organoids in metastasis modeling to replace matrigel |
| title_full_unstemmed | Breast cancer brain metastasis: evaluating the effectiveness of alginate-based organoids in metastasis modeling to replace matrigel |
| title_short | Breast cancer brain metastasis: evaluating the effectiveness of alginate-based organoids in metastasis modeling to replace matrigel |
| title_sort | breast cancer brain metastasis evaluating the effectiveness of alginate based organoids in metastasis modeling to replace matrigel |
| topic | Organogenesis Biomaterials Natural polymers Breast tumo Brain neoplasm |
| url | https://doi.org/10.1186/s13104-025-07154-x |
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